Search results for the GEO ID: GSE30391  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM754184 | GPL570 |
|
WHJSC culture at passage 1, biological rep1
|
WHJSC culture at passage 1
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 1
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754184
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754184/suppl/GSM754184.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754184/suppl/GSM754184.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754185 | GPL570 |
|
WHJSC culture at passage 1, biological rep2
|
WHJSC culture at passage 1
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 1
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754185
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754185/suppl/GSM754185.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754185/suppl/GSM754185.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754186 | GPL570 |
|
WHJSC culture at passage 1, biological rep3
|
WHJSC culture at passage 1
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 1
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754186
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754186/suppl/GSM754186.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754186/suppl/GSM754186.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754187 | GPL570 |
|
WHJSC culture at passage 2, biological rep1
|
WHJSC culture at passage 2
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 2
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754187
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754187/suppl/GSM754187.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754187/suppl/GSM754187.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754188 | GPL570 |
|
WHJSC culture at passage 2, biological rep2
|
WHJSC culture at passage 2
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 2
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754188
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754188/suppl/GSM754188.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754188/suppl/GSM754188.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754189 | GPL570 |
|
WHJSC culture at passage 2, biological rep3
|
WHJSC culture at passage 2
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 2
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754189
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754189/suppl/GSM754189.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754189/suppl/GSM754189.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754190 | GPL570 |
|
WHJSC culture at passage 3, biological rep1
|
WHJSC culture at passage 3
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 3
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754190
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754190/suppl/GSM754190.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754190/suppl/GSM754190.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754191 | GPL570 |
|
WHJSC culture at passage 3, biological rep2
|
WHJSC culture at passage 3
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 3
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754191
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754191/suppl/GSM754191.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754191/suppl/GSM754191.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754192 | GPL570 |
|
WHJSC culture at passage 3, biological rep3
|
WHJSC culture at passage 3
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 3
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754192
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754192/suppl/GSM754192.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754192/suppl/GSM754192.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754193 | GPL570 |
|
WHJSC culture at passage 4, biological rep1
|
WHJSC culture at passage 4
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 4
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754193
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754193/suppl/GSM754193.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754193/suppl/GSM754193.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754194 | GPL570 |
|
WHJSC culture at passage 4, biological rep2
|
WHJSC culture at passage 4
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 4
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754194
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754194/suppl/GSM754194.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754194/suppl/GSM754194.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754195 | GPL570 |
|
WHJSC culture at passage 4, biological rep3
|
WHJSC culture at passage 4
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 4
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754195
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754195/suppl/GSM754195.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754195/suppl/GSM754195.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754196 | GPL570 |
|
WHJSC culture at passage 5, biological rep1
|
WHJSC culture at passage 5
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 5
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754196
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754196/suppl/GSM754196.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754196/suppl/GSM754196.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754197 | GPL570 |
|
WHJSC culture at passage 5, biological rep2
|
WHJSC culture at passage 5
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 5
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754197
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754197/suppl/GSM754197.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754197/suppl/GSM754197.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754198 | GPL570 |
|
WHJSC culture at passage 5, biological rep3
|
WHJSC culture at passage 5
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 5
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754198
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754198/suppl/GSM754198.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754198/suppl/GSM754198.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754199 | GPL570 |
|
WHJSC culture at passage 6, biological rep1
|
WHJSC culture at passage 6
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 6
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754199
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754199/suppl/GSM754199.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754199/suppl/GSM754199.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754200 | GPL570 |
|
WHJSC culture at passage 6, biological rep2
|
WHJSC culture at passage 6
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 6
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754200
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754200/suppl/GSM754200.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754200/suppl/GSM754200.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754201 | GPL570 |
|
WHJSC culture at passage 6, biological rep3
|
WHJSC culture at passage 6
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 6
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754201
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754201/suppl/GSM754201.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754201/suppl/GSM754201.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754202 | GPL570 |
|
WHJSC culture at passage 7, biological rep1
|
WHJSC culture at passage 7
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 7
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754202
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754202/suppl/GSM754202.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754202/suppl/GSM754202.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754203 | GPL570 |
|
WHJSC culture at passage 7, biological rep2
|
WHJSC culture at passage 7
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 7
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754203
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754203/suppl/GSM754203.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754203/suppl/GSM754203.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754204 | GPL570 |
|
WHJSC culture at passage 7, biological rep3
|
WHJSC culture at passage 7
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 7
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754204
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754204/suppl/GSM754204.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754204/suppl/GSM754204.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754205 | GPL570 |
|
WHJSC culture at passage 8, biological rep1
|
WHJSC culture at passage 8
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 8
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754205
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754205/suppl/GSM754205.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754205/suppl/GSM754205.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754206 | GPL570 |
|
WHJSC culture at passage 8, biological rep2
|
WHJSC culture at passage 8
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 8
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754206
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754206/suppl/GSM754206.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754206/suppl/GSM754206.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754207 | GPL570 |
|
WHJSC culture at passage 8, biological rep3
|
WHJSC culture at passage 8
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 8
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754207
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754207/suppl/GSM754207.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754207/suppl/GSM754207.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754208 | GPL570 |
|
WHJSC culture at passage 9, biological rep1
|
WHJSC culture at passage 9
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 9
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754208
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754208/suppl/GSM754208.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754208/suppl/GSM754208.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754209 | GPL570 |
|
WHJSC culture at passage 9, biological rep2
|
WHJSC culture at passage 9
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 9
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754209
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754209/suppl/GSM754209.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754209/suppl/GSM754209.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754210 | GPL570 |
|
WHJSC culture at passage 9, biological rep3
|
WHJSC culture at passage 9
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 9
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754210
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754210/suppl/GSM754210.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754210/suppl/GSM754210.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754211 | GPL570 |
|
WHJSC culture at passage 10, biological rep1
|
WHJSC culture at passage 10
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 10
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754211
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754211/suppl/GSM754211.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754211/suppl/GSM754211.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754212 | GPL570 |
|
WHJSC culture at passage 10, biological rep2
|
WHJSC culture at passage 10
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 10
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754212
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754212/suppl/GSM754212.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754212/suppl/GSM754212.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
GSM754213 | GPL570 |
|
WHJSC culture at passage 10, biological rep3
|
WHJSC culture at passage 10
|
tissue: Umbilical cord Wharton's jelly stem cells culture
passage: 10
|
Gene expression data from WHJSC cultures
|
| Sample_geo_accession | GSM754213
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 05 2011
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from WHJSC fragments using collagenase I. Then, cells were cultured using AMNIOMAX® cell culture medium at 37ºC until subconfluence and furtherly subcultured to new culture flasks until passage 10
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAgen RNAeasy extraction kit, following manufacturer's recommendations
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | MIGUEL,,ALAMINOS
| | Sample_contact_email | alaminom@hotmail.com
| | Sample_contact_institute | UNIVERSITY OF GRANADA
| | Sample_contact_address | AV. MADRID 11
| | Sample_contact_city | GRANADA
| | Sample_contact_zip/postal_code | E18012
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754213/suppl/GSM754213.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM754nnn/GSM754213/suppl/GSM754213.CHP.gz
| | Sample_series_id | GSE30391
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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