Search results for the GEO ID: GSE30473 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM755873 | GPL1261 |
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Hmhi
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Melan-a Hmhi cells starved for 14 days
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cell type: Melan-a Hmhi cells
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Melan-a Hmhi cells starved for 14 days
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Sample_geo_accession | GSM755873
| Sample_status | Public on Jul 03 2012
| Sample_submission_date | Jul 07 2011
| Sample_last_update_date | Jul 03 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were starved in DMEM containing 10% dialyzed FCS, 400 U/ml penicillin, 50 µg/ml streptomycin and, where indicated, were treated with 100 ng/ml EGF for 14 days
| Sample_growth_protocol_ch1 | Cells were kept on 10 cm Petri dishes (Falcon) in DMEM containing 10% FCS, 200 nM TPA, 12 nM cholera toxin, 400 U/ml penicillin, 50 µg/ml streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the miRNeasy kit (Qiagen) according to the manufacturer`s instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and quantile-quantile as normalization method
| Sample_platform_id | GPL1261
| Sample_contact_name | Susanne,Elma,Kneitz
| Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| Sample_contact_phone | +49-931-31 86526
| Sample_contact_department | Physiologigcal Chemistry
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Biozentrum, Am Hubland
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97074
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM755nnn/GSM755873/suppl/GSM755873.CEL.gz
| Sample_series_id | GSE30473
| Sample_data_row_count | 45101
| |
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GSM755874 | GPL1261 |
|
Hmhi + EGF
|
Melan-a Hmhi + 14 days 100 ng/ml EGF
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cell type: Melan-a Hmhi cells
|
Melan-a Hmhi + 14 days 100 ng/ml EGF
|
Sample_geo_accession | GSM755874
| Sample_status | Public on Jul 03 2012
| Sample_submission_date | Jul 07 2011
| Sample_last_update_date | Jul 03 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were starved in DMEM containing 10% dialyzed FCS, 400 U/ml penicillin, 50 µg/ml streptomycin and, where indicated, were treated with 100 ng/ml EGF for 14 days
| Sample_growth_protocol_ch1 | Cells were kept on 10 cm Petri dishes (Falcon) in DMEM containing 10% FCS, 200 nM TPA, 12 nM cholera toxin, 400 U/ml penicillin, 50 µg/ml streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the miRNeasy kit (Qiagen) according to the manufacturer`s instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and quantile-quantile as normalization method
| Sample_platform_id | GPL1261
| Sample_contact_name | Susanne,Elma,Kneitz
| Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| Sample_contact_phone | +49-931-31 86526
| Sample_contact_department | Physiologigcal Chemistry
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Biozentrum, Am Hubland
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97074
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM755nnn/GSM755874/suppl/GSM755874.CEL.gz
| Sample_series_id | GSE30473
| Sample_data_row_count | 45101
| |
|
GSM755875 | GPL1261 |
|
Hmhi-Miz1 kd + EGF
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Melan-a Hmhi-pS-Miz1 + 14 days 100 ng/ml EGF
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cell type: Melan-a Hmhi-Miz1 kd cells
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Melan-a Hmhi-pS-Miz1 + 14 days 100 ng/ml EGF
|
Sample_geo_accession | GSM755875
| Sample_status | Public on Jul 03 2012
| Sample_submission_date | Jul 07 2011
| Sample_last_update_date | Jul 03 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were starved in DMEM containing 10% dialyzed FCS, 400 U/ml penicillin, 50 µg/ml streptomycin and, where indicated, were treated with 100 ng/ml EGF for 14 days
| Sample_growth_protocol_ch1 | Cells were kept on 10 cm Petri dishes (Falcon) in DMEM containing 10% FCS, 200 nM TPA, 12 nM cholera toxin, 400 U/ml penicillin, 50 µg/ml streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the miRNeasy kit (Qiagen) according to the manufacturer`s instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and quantile-quantile as normalization method
| Sample_platform_id | GPL1261
| Sample_contact_name | Susanne,Elma,Kneitz
| Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| Sample_contact_phone | +49-931-31 86526
| Sample_contact_department | Physiologigcal Chemistry
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Biozentrum, Am Hubland
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97074
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM755nnn/GSM755875/suppl/GSM755875.CEL.gz
| Sample_series_id | GSE30473
| Sample_data_row_count | 45101
| |
|
GSM755876 | GPL1261 |
|
Hmhi-MYC-TA + EGF
|
Melan-a Hmhi-MYC-TA + 14 days 100 ng/ml EGF
|
cell type: Melan-a Hmhi-MYC-TA cells
|
Melan-a Hmhi-MYC-TA + 14 days 100 ng/ml EGF
|
Sample_geo_accession | GSM755876
| Sample_status | Public on Jul 03 2012
| Sample_submission_date | Jul 07 2011
| Sample_last_update_date | Jul 03 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were starved in DMEM containing 10% dialyzed FCS, 400 U/ml penicillin, 50 µg/ml streptomycin and, where indicated, were treated with 100 ng/ml EGF for 14 days
| Sample_growth_protocol_ch1 | Cells were kept on 10 cm Petri dishes (Falcon) in DMEM containing 10% FCS, 200 nM TPA, 12 nM cholera toxin, 400 U/ml penicillin, 50 µg/ml streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the miRNeasy kit (Qiagen) according to the manufacturer`s instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and quantile-quantile as normalization method
| Sample_platform_id | GPL1261
| Sample_contact_name | Susanne,Elma,Kneitz
| Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| Sample_contact_phone | +49-931-31 86526
| Sample_contact_department | Physiologigcal Chemistry
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Biozentrum, Am Hubland
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97074
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM755nnn/GSM755876/suppl/GSM755876.CEL.gz
| Sample_series_id | GSE30473
| Sample_data_row_count | 45101
| |
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