Search results for the GEO ID: GSE30478  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM756005 | GPL570 |
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Scr_shRNA
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colorectal carcinoma cells, scrambled shRNA control
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cell line: HCT116
cell type: colorectal carcinoma cells
shRNA: scrambled shRNA
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Gene expression data from HCT116 cells treated with scramble shRNA and selected with puromycin.
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| Sample_geo_accession | GSM756005
| | Sample_status | Public on Jul 07 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HCT116 cells were infected with Scr shRNA, UHRF1 shRNA-2 or UHRF1 shRNA-5 containing lentivirus and harvested after 6-days selection with puromycin.
| | Sample_growth_protocol_ch1 | HCT116 cells were cultured with McCoy’s 5A medium containing 10% fetal bovine serum (FBS; Hyclone) and 1% Pen-Strep.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNase I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_data_processing | The data were analyzed with Expression Console software (RMA), which is produced by Affymetrix.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756005/suppl/GSM756005.CEL.gz
| | Sample_series_id | GSE30478
| | Sample_data_row_count | 54613
| | |
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GSM756006 | GPL570 |
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UHRF1_sh2
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colorectal carcinoma cells, UHRF1 shRNA
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cell line: HCT116
cell type: colorectal carcinoma cells
shRNA: UHRF1 shRNA-2
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Gene expression data from HCT116 cells treated with UHRF1 shRNA-2 and selected with puromycin.
|
| Sample_geo_accession | GSM756006
| | Sample_status | Public on Jul 07 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HCT116 cells were infected with Scr shRNA, UHRF1 shRNA-2 or UHRF1 shRNA-5 containing lentivirus and harvested after 6-days selection with puromycin.
| | Sample_growth_protocol_ch1 | HCT116 cells were cultured with McCoy’s 5A medium containing 10% fetal bovine serum (FBS; Hyclone) and 1% Pen-Strep.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNase I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_data_processing | The data were analyzed with Expression Console software (RMA), which is produced by Affymetrix.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756006/suppl/GSM756006.CEL.gz
| | Sample_series_id | GSE30478
| | Sample_data_row_count | 54613
| | |
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GSM756007 | GPL570 |
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UHRF1_sh5
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colorectal carcinoma cells, UHRF1 shRNA
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cell line: HCT116
cell type: colorectal carcinoma cells
shRNA: UHRF1 shRNA-5
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Gene expression data from HCT116 cells treated with UHRF1 shRNA-5 and selected with puromycin.
|
| Sample_geo_accession | GSM756007
| | Sample_status | Public on Jul 07 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HCT116 cells were infected with Scr shRNA, UHRF1 shRNA-2 or UHRF1 shRNA-5 containing lentivirus and harvested after 6-days selection with puromycin.
| | Sample_growth_protocol_ch1 | HCT116 cells were cultured with McCoy’s 5A medium containing 10% fetal bovine serum (FBS; Hyclone) and 1% Pen-Strep.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNase I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com).
| | Sample_data_processing | The data were analyzed with Expression Console software (RMA), which is produced by Affymetrix.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756007/suppl/GSM756007.CEL.gz
| | Sample_series_id | GSE30478
| | Sample_data_row_count | 54613
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