Search results for the GEO ID: GSE30494  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM756414 | GPL570 |
|
A549-siEGFP-1
|
A549
|
cell line: A549
transfector: siEGFP
|
|
| Sample_geo_accession | GSM756414
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756414/suppl/GSM756414.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756415 | GPL570 |
|
A549-siEGFP-2
|
A549
|
cell line: A549
transfector: siEGFP
|
|
| Sample_geo_accession | GSM756415
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756415/suppl/GSM756415.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756416 | GPL570 |
|
A549-siFFluc-1
|
A549
|
cell line: A549
transfector: siFFluc
|
|
| Sample_geo_accession | GSM756416
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756416/suppl/GSM756416.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756417 | GPL570 |
|
A549-siFFluc-2
|
A549
|
cell line: A549
transfector: siFFluc
|
|
| Sample_geo_accession | GSM756417
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756417/suppl/GSM756417.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756418 | GPL570 |
|
A549-siKDM3A-1
|
A549
|
cell line: A549
transfector: siKDM3A
|
|
| Sample_geo_accession | GSM756418
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756418/suppl/GSM756418.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756419 | GPL570 |
|
A549-siKDM3A-2
|
A549
|
cell line: A549
transfector: siKDM3A
|
|
| Sample_geo_accession | GSM756419
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756419/suppl/GSM756419.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756420 | GPL570 |
|
SW780-siEGFP-1
|
SW780
|
cell line: SW780
transfector: siEGFP
|
|
| Sample_geo_accession | GSM756420
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756420/suppl/GSM756420.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756421 | GPL570 |
|
SW780-siEGFP-2
|
SW780
|
cell line: SW780
transfector: siEGFP
|
|
| Sample_geo_accession | GSM756421
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756421/suppl/GSM756421.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756422 | GPL570 |
|
SW780-siFFluc-1
|
SW780
|
cell line: SW780
transfector: siFFluc
|
|
| Sample_geo_accession | GSM756422
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756422/suppl/GSM756422.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756423 | GPL570 |
|
SW780-siFFluc-2
|
SW780
|
cell line: SW780
transfector: siFFluc
|
|
| Sample_geo_accession | GSM756423
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756423/suppl/GSM756423.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756424 | GPL570 |
|
SW780-siKDM3A-1
|
SW780
|
cell line: SW780
transfector: siKDM3A
|
|
| Sample_geo_accession | GSM756424
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756424/suppl/GSM756424.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
| | |
|
GSM756425 | GPL570 |
|
SW780-siKDM3A-2
|
SW780
|
cell line: SW780
transfector: siKDM3A
|
|
| Sample_geo_accession | GSM756425
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | siRNA duplexes (100 nM final concentration) were transfected in cancer cell lines with lipofectamine 2000 (Invitrogen) for 24 h
| | Sample_growth_protocol_ch1 | A549 (RPMI1640) / SW780 (Leibovitz’s L-15) cell lines supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained at 37 degree in humid air with/ without 5% CO2 condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hyun-soo,,Cho
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | 4-6-1, shirokanedai, minatoku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 108-8369
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756425/suppl/GSM756425.CEL.gz
| | Sample_series_id | GSE30494
| | Sample_data_row_count | 54675
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