Search results for the GEO ID: GSE30499  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM756510 | GPL570 |
|
U2OS_Control_0hr
|
U2OS cells, control, 0 hr
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: control
treatment duration: 3 hrs
sampling time point: 0 hr
|
Gene expression data of U2OS cells grown in DMEM/10% FCS.
|
| Sample_geo_accession | GSM756510
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756510/suppl/GSM756510.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756511 | GPL570 |
|
U2OS_Control_1.5hr
|
U2OScells, control, 1.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: control
treatment duration: 3 hrs
sampling time point: 1.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml of DRB for 1.5 hr.
|
| Sample_geo_accession | GSM756511
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756511/suppl/GSM756511.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756512 | GPL570 |
|
U2OS_Control_3hr
|
U2OScells, control, 3 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: control
treatment duration: 3 hrs
sampling time point: 3 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml of DRB for 3 hr.
|
| Sample_geo_accession | GSM756512
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756512/suppl/GSM756512.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756513 | GPL570 |
|
U2OS_Control_4.5hr
|
U2OScells, control, 4.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: control
treatment duration: 3 hrs
sampling time point: 4.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml of DRB for 4.5 hr.
|
| Sample_geo_accession | GSM756513
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756513/suppl/GSM756513.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756514 | GPL570 |
|
U2OS_Hypoxia_0hr
|
U2OScells, hypoxia, 0 hr
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: hypoxia
treatment duration: 3 hrs
sampling time point: 0 hr
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, rendered hypoxic (<0.1% Oxygen for 3 hrs).
|
| Sample_geo_accession | GSM756514
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756514/suppl/GSM756514.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756515 | GPL570 |
|
U2OS_Hypoxia_1.5hr
|
U2OScells, hypoxia, 1.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: hypoxia
treatment duration: 3 hrs
sampling time point: 1.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, rendered hypoxic (<0.1% Oxygen for 3 hrs), treated with 100 ug/ml of DRB for 1.5 hr.
|
| Sample_geo_accession | GSM756515
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756515/suppl/GSM756515.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756516 | GPL570 |
|
U2OS_Hypoxia_3hr
|
U2OScells, hypoxia, 3 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: hypoxia
treatment duration: 3 hrs
sampling time point: 3 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, rendered hypoxic (<0.1% Oxygen for 3 hrs), treated with 100 ug/ml of DRB for 3 hr.
|
| Sample_geo_accession | GSM756516
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756516/suppl/GSM756516.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756517 | GPL570 |
|
U2OS_Hypoxia_4.5hr
|
U2OScells, hypoxia, 4.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: hypoxia
treatment duration: 3 hrs
sampling time point: 4.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, rendered hypoxic (<0.1% Oxygen for 3 hrs), treated with 100 ug/ml of DRB for 4.5 hr.
|
| Sample_geo_accession | GSM756517
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756517/suppl/GSM756517.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756518 | GPL570 |
|
U2OS_Tunicamycin_0hr
|
U2OScells, tunicamycin, 0 hr
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: tunicamycin
treatment duration: 3 hrs
sampling time point: 0 hr
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 2.5 ug/ml tunicamycin for 3 hrs.
|
| Sample_geo_accession | GSM756518
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756518/suppl/GSM756518.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756519 | GPL570 |
|
U2OS_Tunicamycin_1.5hr
|
U2OScells, tunicamycin, 1.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: tunicamycin
treatment duration: 3 hrs
sampling time point: 1.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 2.5 ug/ml tunicamycin for 3 hrs treated with 100 ug/ml of DRB for 1.5 hr.
|
| Sample_geo_accession | GSM756519
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756519/suppl/GSM756519.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756520 | GPL570 |
|
U2OS_Tunicamycin_3hr
|
U2OScells, tunicamycin, 3 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: tunicamycin
treatment duration: 3 hrs
sampling time point: 3 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 2.5 ug/ml tunicamycin for 3 hrs treated with 100 ug/ml of DRB for 3 hr.
|
| Sample_geo_accession | GSM756520
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756520/suppl/GSM756520.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756521 | GPL570 |
|
U2OS_Tunicamycin_4.5hr
|
U2OScells, tunicamycin, 4.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: tunicamycin
treatment duration: 3 hrs
sampling time point: 4.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 2.5 ug/ml tunicamycin for 3 hrs treated with 100 ug/ml of DRB for 4.5 hr.
|
| Sample_geo_accession | GSM756521
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756521/suppl/GSM756521.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756522 | GPL570 |
|
U2OS_emetine_0hr
|
U2OScells, emetine, 0 hr
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: emetine
treatment duration: 3 hrs
sampling time point: 0 hr
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml emetine for 3 hrs.
|
| Sample_geo_accession | GSM756522
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756522/suppl/GSM756522.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756523 | GPL570 |
|
U2OS_emetine_1.5hr
|
U2OScells, emetine, 1.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: emetine
treatment duration: 3 hrs
sampling time point: 1.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml emetine for 3 hrs, treated with 100 ug/ml of DRB for 1.5 hr.
|
| Sample_geo_accession | GSM756523
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756523/suppl/GSM756523.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756524 | GPL570 |
|
U2OS_emetine_3hr
|
U2OScells, emetine, 3 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: emetine
treatment duration: 3 hrs
sampling time point: 3 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml emetine for 3 hrs, treated with 100 ug/ml of DRB for 3 hr.
|
| Sample_geo_accession | GSM756524
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756524/suppl/GSM756524.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756525 | GPL570 |
|
U2OS_emetine_4.5hr
|
U2OScells, emetine, 4.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: emetine
treatment duration: 3 hrs
sampling time point: 4.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, treated with 100 ug/ml emetine for 3 hrs, treated with 100 ug/ml of DRB for 4.5 hr.
|
| Sample_geo_accession | GSM756525
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756525/suppl/GSM756525.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756526 | GPL570 |
|
U2OS_shRent1_Upf1_0hr
|
U2OScells, shRent1, 0 hr
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: shRent1
treatment duration: 3 hrs
sampling time point: 0 hr
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, with shUpf1/Rent1.
|
| Sample_geo_accession | GSM756526
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756526/suppl/GSM756526.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756527 | GPL570 |
|
U2OS_shRent1_Upf1_1.5hr
|
U2OScells, shRent1, 1.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: shRent1
treatment duration: 3 hrs
sampling time point: 1.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, with shUpf1/Rent1 treated with 100 ug/ml of DRB for 1.5 hr.
|
| Sample_geo_accession | GSM756527
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756527/suppl/GSM756527.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756528 | GPL570 |
|
U2OS_shRent1_Upf1_3hr
|
U2OScells, shRent1, 3 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: shRent1
treatment duration: 3 hrs
sampling time point: 3 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, with shUpf1/Rent1 treated with 100 ug/ml of DRB for 3 hr.
|
| Sample_geo_accession | GSM756528
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756528/suppl/GSM756528.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
GSM756529 | GPL570 |
|
U2OS_shRent1_Upf1_4.5hr
|
U2OScells, shRent1, 4.5 hrs
|
cell line: U2OS
cell type: osteosarcoma cells
treatment: shRent1
treatment duration: 3 hrs
sampling time point: 4.5 hrs
|
Gene expression data of U2OS cells grown in DMEM/10% FCS, with shUpf1/Rent1 treated with 100 ug/ml of DRB for 4.5 hr.
|
| Sample_geo_accession | GSM756529
| | Sample_status | Public on Jul 08 2011
| | Sample_submission_date | Jul 07 2011
| | Sample_last_update_date | Jul 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the specific stresses for three hours, and then with the pol II elongation inhibitor DRB. RNA was collected at 0 hrs or the indicated time points after DRB addition.
| | Sample_growth_protocol_ch1 | U2OS cells (osteosarcoma) were grown in DMEM (4.5 g/dl glucose) with 10% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the ABI cDNA kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit.
| | Sample_hyb_protocol | Labeled and fragmented cRNA was hybridized to HG-U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix 7G GeneArray scanner.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using the Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756529/suppl/GSM756529.CEL.gz
| | Sample_series_id | GSE30499
| | Sample_data_row_count | 54675
| | |
|
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