Search results for the GEO ID: GSE30516 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM756853 | GPL570 |
|
Part 1: BT20 0 minute time point rep1
|
BT20 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756853
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756853/suppl/GSM756853_AA215_1.1.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756854 | GPL570 |
|
Part 1: BT20 0 minute time point rep2
|
BT20 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756854
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756854/suppl/GSM756854_AA216_1.2.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756855 | GPL570 |
|
Part 1: BT20 0 minute time point rep3
|
BT20 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756855
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756855/suppl/GSM756855_AA217_3_1.3.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756856 | GPL570 |
|
Part 1: BT20 30 minute time point rep1
|
BT20 cells, 30 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 30 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756856
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756856/suppl/GSM756856_AA218_3_2.1.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756857 | GPL570 |
|
Part 1: BT20 30 minute time point rep2
|
BT20 cells, 30 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 30 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756857
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756857/suppl/GSM756857_AA219_2.2.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756858 | GPL570 |
|
Part 1: BT20 30 minute time point rep3
|
BT20 cells, 30 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 30 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756858
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756858/suppl/GSM756858_AA220_2.3.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756859 | GPL570 |
|
Part 1: BT20 1 day time point rep1
|
BT20 cells, 24 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756859
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756859/suppl/GSM756859_AA221_3.1.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756860 | GPL570 |
|
Part 1: BT20 1 day time point rep2
|
BT20 cells, 24 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756860
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756860/suppl/GSM756860_AA222_3.2.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756861 | GPL570 |
|
Part 1: BT20 1 day time point rep3
|
BT20 cells, 24 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756861
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756861/suppl/GSM756861_AA223_3.3.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756862 | GPL570 |
|
Part 1: BT20 0 minute time point rep4
|
BT20 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756862
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756862/suppl/GSM756862_AA283_BTOA.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756863 | GPL570 |
|
Part 1: BT20 6 hour time point rep1
|
BT20 cells, 6 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 6 hours
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756863
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756863/suppl/GSM756863_AA284_BT6A.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756864 | GPL570 |
|
Part 1: BT20 6 hour time point rep2
|
BT20 cells, 6 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 6 hours
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756864
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756864/suppl/GSM756864_AA285_BT6B.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756865 | GPL570 |
|
Part 1: BT20 6 hour time point rep3
|
BT20 cells, 6 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 6 hours
treatment: 10 µM erlotinib
cell line: BT20
|
|
Sample_geo_accession | GSM756865
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756865/suppl/GSM756865_AA286_BT6C.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756866 | GPL570 |
|
Part 1: MD 0 minute time point rep1
|
MDA-MB-453 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: MDA-MB-453
|
|
Sample_geo_accession | GSM756866
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756866/suppl/GSM756866_AA287_MD0A.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756867 | GPL570 |
|
Part 1: MD 0 minute time point rep2
|
MDA-MB-453 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: MDA-MB-453
|
|
Sample_geo_accession | GSM756867
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756867/suppl/GSM756867_AA288_MD0B.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756868 | GPL570 |
|
Part 1: MD 0 minute time point rep3
|
MDA-MB-453 cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: MDA-MB-453
|
|
Sample_geo_accession | GSM756868
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756868/suppl/GSM756868_AA289_MD0C.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756869 | GPL570 |
|
Part 1: MD 1 day time point rep1
|
MDA-MB-453 cells, 24 hours post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: MDA-MB-453
|
|
Sample_geo_accession | GSM756869
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756869/suppl/GSM756869_AA290_MD24A.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756870 | GPL570 |
|
Part 1: MD 1 day time point rep2
|
MDA-MB-453 cells, 24 hours post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: MDA-MB-453
|
|
Sample_geo_accession | GSM756870
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756870/suppl/GSM756870_AA291_MD24B.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756871 | GPL570 |
|
Part 1: MD 1 day time point rep3
|
MDA-MB-453 cells, 24 hours post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: MDA-MB-453
|
|
Sample_geo_accession | GSM756871
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756871/suppl/GSM756871_AA292_MD24C.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756872 | GPL570 |
|
Part 2: MCF 0 minute time point rep1
|
MCF cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: MCF
|
|
Sample_geo_accession | GSM756872
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756872/suppl/GSM756872_AB025_MCF_0A.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756873 | GPL570 |
|
Part 2: MCF 0 minute time point rep2
|
MCF cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: MCF
|
|
Sample_geo_accession | GSM756873
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756873/suppl/GSM756873_AB026_MCF_0B.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756874 | GPL570 |
|
Part 2: MCF 0 minute time point rep3
|
MCF cells, 0 minutes post treatment with 10µM concentration of erlotinib
|
time post treatment: 0 minutes
treatment: 10 µM erlotinib
cell line: MCF
|
|
Sample_geo_accession | GSM756874
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756874/suppl/GSM756874_AB027_MCF_0C.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756875 | GPL570 |
|
Part 2: MCF 1 day time point rep1
|
MCF cells, 24 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: MCF
|
|
Sample_geo_accession | GSM756875
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756875/suppl/GSM756875_AB028_MCF_24A.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
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GSM756876 | GPL570 |
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Part 2: MCF 1 day time point rep2
|
MCF cells, 24 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: MCF
|
|
Sample_geo_accession | GSM756876
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756876/suppl/GSM756876_AB029_MCF_24B.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
GSM756877 | GPL570 |
|
Part 2: MCF 1 day time point rep3
|
MCF cells, 24 hour post treatment with 10µM concentration of erlotinib
|
time post treatment: 24 hours
treatment: 10 µM erlotinib
cell line: MCF
|
|
Sample_geo_accession | GSM756877
| Sample_status | Public on May 15 2012
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | May 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erlotinib was added to a final concentration of 10µM
| Sample_growth_protocol_ch1 | 1x10^6 cells were plated in a 10 cM dish in media containing 10% FBS, and allowed to adhere for 24 hours before treatment with erlotinib.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
| Sample_platform_id | GPL570
| Sample_contact_name | Charles,Arthur,Whittaker
| Sample_contact_email | charliew@mit.edu
| Sample_contact_institute | Koch Institute
| Sample_contact_address | 77 Mass Ave 76-189
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02152
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756877/suppl/GSM756877_AB030_MCF_24C.CEL.gz
| Sample_series_id | GSE30516
| Sample_data_row_count | 54613
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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