Search results for the GEO ID: GSE30524 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM756954 | GPL570 |
|
untreated 6h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (untreated)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 6 hrs untreated
|
Sample_geo_accession | GSM756954
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756954/suppl/GSM756954_untreated_6h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756955 | GPL570 |
|
untreated 24h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (untreated)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 24 hrs untreated
|
Sample_geo_accession | GSM756955
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756955/suppl/GSM756955_untreated_24h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756956 | GPL570 |
|
PPP 6h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (treated with PPP)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 6 hrs treated with PPP
|
Sample_geo_accession | GSM756956
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756956/suppl/GSM756956_PPP_6h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756957 | GPL570 |
|
PPP 24h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (treated with PPP)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 24 hrs treated with PPP
|
Sample_geo_accession | GSM756957
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756957/suppl/GSM756957_PPP_24h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756958 | GPL570 |
|
LBH 6h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (treated with LBH589)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 6 hrs treated with LBH589
|
Sample_geo_accession | GSM756958
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756958/suppl/GSM756958_LBH_6h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756959 | GPL570 |
|
LBH 24h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (treated with LBH589)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 24 hrs treated with LBH589
|
Sample_geo_accession | GSM756959
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756959/suppl/GSM756959_LBH_24h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756960 | GPL570 |
|
Combo 6h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (treated with combo LBH589 & PPP)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 6 hrs treated with Combo
|
Sample_geo_accession | GSM756960
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756960/suppl/GSM756960_Combo_6h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
|
GSM756961 | GPL570 |
|
Combo 24h, biological rep 1
|
Multiple myeloma cell line RPMI 8266 (treated with combo LBH589 & PPP)
|
cell line: RPMI 8266
cell type: B lymphocyte
gender: Male
|
Gene Expression Data from RPMI 8266 24 hrs treated with Combo
|
Sample_geo_accession | GSM756961
| Sample_status | Public on Jul 09 2011
| Sample_submission_date | Jul 08 2011
| Sample_last_update_date | Jul 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RPMI 8266 cells were treated with LBH589 (20nM), picropodophyllin (PPP)(0.375uM) , and the combination of these two drugs (same concentrations).
| Sample_growth_protocol_ch1 | RMPI 8266 were grown in RPMI1640 and 10% FCS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 250 nanogram total RNA of each sample according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Hybridization was done for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA)
| Sample_scan_protocol | Scanning was done using GeneChip® Scanner 3000 7G
| Sample_data_processing | Data analysis and normalization done using GeneSpring v11. RMA and quantile normalization was used to normalized the data.
| Sample_platform_id | GPL570
| Sample_contact_name | Prasoon,,Agarwal
| Sample_contact_email | prasoon.agarwal@igp.uu.se
| Sample_contact_department | IGP
| Sample_contact_institute | Rudbeck Laboratory, Uppsala University
| Sample_contact_address | Dag Hammarshkojlds vag 20
| Sample_contact_city | Uppsala
| Sample_contact_state | Sweden
| Sample_contact_zip/postal_code | 75185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756961/suppl/GSM756961_Combo_24h.CEL.gz
| Sample_series_id | GSE30524
| Sample_data_row_count | 54613
| |
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