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Search results for the GEO ID: GSE30526

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Description

Characteristics

GSM756974

GPL1355

siRNA treated, biological rep1

Insulinoma 832/13 INS-1 cell line

passage: between passages 7 and 25 cell line: Insulinoma 832/13 INS-1 cell line treatment: COUP-TFII knockdown

Sample_geo_accession	GSM756974
Sample_status	Public on Oct 07 2011
Sample_submission_date	Jul 08 2011
Sample_last_update_date	Oct 07 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	COUP-TFII depletion experiments in the 832/13 INS-1 cell line were performed as described previously (Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M (2008). The transcription factor COUP-TFII is negatively regulated by insulin and glucose via FoxO1 and ChREBP controlled pathways. . Mol Cell Biol 28: 6568-6579). Briefly, cells were transfected with COUP-TFII siRNA or a scrambled siRNA using a Amaxa device following manufacturer instructions.
Sample_growth_protocol_ch1	The rat insulinoma 832/13 INS-1 cell line was used between passages 7 and 25. Cells were cultured at 5% CO2–95% air at 37°C in RPMI 640 medium containing 11 mM D-glucose supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin-streptomycin, 10 mM HEPES, 1 mM sodium pyruvate (Invitrogen), and 50 μM β-mercaptoethanol (Invitrogen) (INS-1 medium).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified from cultured cells using the RNA-Plus reagent according to the manufacturer’s instructions (Q-BIOgene).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 1 ug total RNA (Expression Analysis Technical Manual, 701025 Rev5, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on 230 2.0 rat Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the affymetrix 3000 genescanner
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150
Sample_platform_id	GPL1355
Sample_contact_name	Leentje,,Van Lommel
Sample_contact_laboratory	gene expression unit
Sample_contact_department	Molecular cell biology
Sample_contact_institute	KULeuven
Sample_contact_address	Herestraat 49 bus 901
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756974/suppl/GSM756974_A14sirna.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756974/suppl/GSM756974_A14sirna.CHP.gz
Sample_series_id	GSE30526
Sample_data_row_count	31099

GSM756975

GPL1355

siRNA treated, biological rep2

Insulinoma 832/13 INS-1 cell line

passage: between passages 7 and 25 cell line: Insulinoma 832/13 INS-1 cell line treatment: COUP-TFII knockdown

Sample_geo_accession	GSM756975
Sample_status	Public on Oct 07 2011
Sample_submission_date	Jul 08 2011
Sample_last_update_date	Oct 07 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	COUP-TFII depletion experiments in the 832/13 INS-1 cell line were performed as described previously (Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M (2008). The transcription factor COUP-TFII is negatively regulated by insulin and glucose via FoxO1 and ChREBP controlled pathways. . Mol Cell Biol 28: 6568-6579). Briefly, cells were transfected with COUP-TFII siRNA or a scrambled siRNA using a Amaxa device following manufacturer instructions.
Sample_growth_protocol_ch1	The rat insulinoma 832/13 INS-1 cell line was used between passages 7 and 25. Cells were cultured at 5% CO2–95% air at 37°C in RPMI 640 medium containing 11 mM D-glucose supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin-streptomycin, 10 mM HEPES, 1 mM sodium pyruvate (Invitrogen), and 50 μM β-mercaptoethanol (Invitrogen) (INS-1 medium).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified from cultured cells using the RNA-Plus reagent according to the manufacturer’s instructions (Q-BIOgene).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 1 ug total RNA (Expression Analysis Technical Manual, 701025 Rev5, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on 230 2.0 rat Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the affymetrix 3000 genescanner
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150
Sample_platform_id	GPL1355
Sample_contact_name	Leentje,,Van Lommel
Sample_contact_laboratory	gene expression unit
Sample_contact_department	Molecular cell biology
Sample_contact_institute	KULeuven
Sample_contact_address	Herestraat 49 bus 901
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756975/suppl/GSM756975_A25sirna.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756975/suppl/GSM756975_A25sirna.CHP.gz
Sample_series_id	GSE30526
Sample_data_row_count	31099

GSM756976

GPL1355

siRNA treated, biological rep3

Insulinoma 832/13 INS-1 cell line

passage: between passages 7 and 25 cell line: Insulinoma 832/13 INS-1 cell line treatment: COUP-TFII knockdown

Sample_geo_accession	GSM756976
Sample_status	Public on Oct 07 2011
Sample_submission_date	Jul 08 2011
Sample_last_update_date	Oct 07 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	COUP-TFII depletion experiments in the 832/13 INS-1 cell line were performed as described previously (Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M (2008). The transcription factor COUP-TFII is negatively regulated by insulin and glucose via FoxO1 and ChREBP controlled pathways. . Mol Cell Biol 28: 6568-6579). Briefly, cells were transfected with COUP-TFII siRNA or a scrambled siRNA using a Amaxa device following manufacturer instructions.
Sample_growth_protocol_ch1	The rat insulinoma 832/13 INS-1 cell line was used between passages 7 and 25. Cells were cultured at 5% CO2–95% air at 37°C in RPMI 640 medium containing 11 mM D-glucose supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin-streptomycin, 10 mM HEPES, 1 mM sodium pyruvate (Invitrogen), and 50 μM β-mercaptoethanol (Invitrogen) (INS-1 medium).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified from cultured cells using the RNA-Plus reagent according to the manufacturer’s instructions (Q-BIOgene).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 1 ug total RNA (Expression Analysis Technical Manual, 701025 Rev5, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on 230 2.0 rat Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the affymetrix 3000 genescanner
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150
Sample_platform_id	GPL1355
Sample_contact_name	Leentje,,Van Lommel
Sample_contact_laboratory	gene expression unit
Sample_contact_department	Molecular cell biology
Sample_contact_institute	KULeuven
Sample_contact_address	Herestraat 49 bus 901
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756976/suppl/GSM756976_A64sirna.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756976/suppl/GSM756976_A64sirna.CHP.gz
Sample_series_id	GSE30526
Sample_data_row_count	31099

GSM756977

GPL1355

INS1 control, biological rep1

Insulinoma 832/13 INS-1 cell line

passage: between passages 7 and 25 cell line: Insulinoma 832/13 INS-1 cell line treatment: scrambled

Sample_geo_accession	GSM756977
Sample_status	Public on Oct 07 2011
Sample_submission_date	Jul 08 2011
Sample_last_update_date	Oct 07 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	COUP-TFII depletion experiments in the 832/13 INS-1 cell line were performed as described previously (Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M (2008). The transcription factor COUP-TFII is negatively regulated by insulin and glucose via FoxO1 and ChREBP controlled pathways. . Mol Cell Biol 28: 6568-6579). Briefly, cells were transfected with COUP-TFII siRNA or a scrambled siRNA using a Amaxa device following manufacturer instructions.
Sample_growth_protocol_ch1	The rat insulinoma 832/13 INS-1 cell line was used between passages 7 and 25. Cells were cultured at 5% CO2–95% air at 37°C in RPMI 640 medium containing 11 mM D-glucose supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin-streptomycin, 10 mM HEPES, 1 mM sodium pyruvate (Invitrogen), and 50 μM β-mercaptoethanol (Invitrogen) (INS-1 medium).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified from cultured cells using the RNA-Plus reagent according to the manufacturer’s instructions (Q-BIOgene).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 1 ug total RNA (Expression Analysis Technical Manual, 701025 Rev5, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on 230 2.0 rat Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the affymetrix 3000 genescanner
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150
Sample_platform_id	GPL1355
Sample_contact_name	Leentje,,Van Lommel
Sample_contact_laboratory	gene expression unit
Sample_contact_department	Molecular cell biology
Sample_contact_institute	KULeuven
Sample_contact_address	Herestraat 49 bus 901
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756977/suppl/GSM756977_A13wt.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756977/suppl/GSM756977_A13wt.CHP.gz
Sample_series_id	GSE30526
Sample_data_row_count	31099

GSM756978

GPL1355

INS1 control, bioological rep2

Insulinoma 832/13 INS-1 cell line

passage: between passages 7 and 25 cell line: Insulinoma 832/13 INS-1 cell line treatment: scrambled

Sample_geo_accession	GSM756978
Sample_status	Public on Oct 07 2011
Sample_submission_date	Jul 08 2011
Sample_last_update_date	Oct 07 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	COUP-TFII depletion experiments in the 832/13 INS-1 cell line were performed as described previously (Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M (2008). The transcription factor COUP-TFII is negatively regulated by insulin and glucose via FoxO1 and ChREBP controlled pathways. . Mol Cell Biol 28: 6568-6579). Briefly, cells were transfected with COUP-TFII siRNA or a scrambled siRNA using a Amaxa device following manufacturer instructions.
Sample_growth_protocol_ch1	The rat insulinoma 832/13 INS-1 cell line was used between passages 7 and 25. Cells were cultured at 5% CO2–95% air at 37°C in RPMI 640 medium containing 11 mM D-glucose supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin-streptomycin, 10 mM HEPES, 1 mM sodium pyruvate (Invitrogen), and 50 μM β-mercaptoethanol (Invitrogen) (INS-1 medium).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified from cultured cells using the RNA-Plus reagent according to the manufacturer’s instructions (Q-BIOgene).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 1 ug total RNA (Expression Analysis Technical Manual, 701025 Rev5, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on 230 2.0 rat Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the affymetrix 3000 genescanner
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150
Sample_platform_id	GPL1355
Sample_contact_name	Leentje,,Van Lommel
Sample_contact_laboratory	gene expression unit
Sample_contact_department	Molecular cell biology
Sample_contact_institute	KULeuven
Sample_contact_address	Herestraat 49 bus 901
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756978/suppl/GSM756978_A22wt.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756978/suppl/GSM756978_A22wt.CHP.gz
Sample_series_id	GSE30526
Sample_data_row_count	31099

GSM756979

GPL1355

INS1 control, biological rep3

Insulinoma 832/13 INS-1 cell line

passage: between passages 7 and 25 cell line: Insulinoma 832/13 INS-1 cell line treatment: scrambled

Sample_geo_accession	GSM756979
Sample_status	Public on Oct 07 2011
Sample_submission_date	Jul 08 2011
Sample_last_update_date	Oct 07 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	COUP-TFII depletion experiments in the 832/13 INS-1 cell line were performed as described previously (Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M (2008). The transcription factor COUP-TFII is negatively regulated by insulin and glucose via FoxO1 and ChREBP controlled pathways. . Mol Cell Biol 28: 6568-6579). Briefly, cells were transfected with COUP-TFII siRNA or a scrambled siRNA using a Amaxa device following manufacturer instructions.
Sample_growth_protocol_ch1	The rat insulinoma 832/13 INS-1 cell line was used between passages 7 and 25. Cells were cultured at 5% CO2–95% air at 37°C in RPMI 640 medium containing 11 mM D-glucose supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin-streptomycin, 10 mM HEPES, 1 mM sodium pyruvate (Invitrogen), and 50 μM β-mercaptoethanol (Invitrogen) (INS-1 medium).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified from cultured cells using the RNA-Plus reagent according to the manufacturer’s instructions (Q-BIOgene).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 1 ug total RNA (Expression Analysis Technical Manual, 701025 Rev5, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on 230 2.0 rat Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the affymetrix 3000 genescanner
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150
Sample_platform_id	GPL1355
Sample_contact_name	Leentje,,Van Lommel
Sample_contact_laboratory	gene expression unit
Sample_contact_department	Molecular cell biology
Sample_contact_institute	KULeuven
Sample_contact_address	Herestraat 49 bus 901
Sample_contact_city	Leuven
Sample_contact_zip/postal_code	3000
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756979/suppl/GSM756979_A62wt.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM756nnn/GSM756979/suppl/GSM756979_A62wt.CHP.gz
Sample_series_id	GSE30526
Sample_data_row_count	31099

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