Search results for the GEO ID: GSE30532  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM757143 | GPL1355 |
|
Sugar, biological rep1
|
rats fed sugar mix syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: sugar
|
|
| Sample_geo_accession | GSM757143
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757143/suppl/GSM757143_Suger_4.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757144 | GPL1355 |
|
Sugar, biological rep2
|
rats fed sugar mix syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: sugar
|
|
| Sample_geo_accession | GSM757144
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757144/suppl/GSM757144_Sugar_6.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757145 | GPL1355 |
|
Sugar, biological rep3
|
rats fed sugar mix syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: sugar
|
|
| Sample_geo_accession | GSM757145
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757145/suppl/GSM757145_Suger_8.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757146 | GPL1355 |
|
Sugar, biological rep4
|
rats fed sugar mix syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: sugar
|
|
| Sample_geo_accession | GSM757146
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757146/suppl/GSM757146_Sugar_12.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757147 | GPL1355 |
|
Sugar, biological rep5
|
rats fed sugar mix syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: sugar
|
|
| Sample_geo_accession | GSM757147
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757147/suppl/GSM757147_Suger_14.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757148 | GPL1355 |
|
Sugar, biological rep6
|
rats fed sugar mix syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: sugar
|
|
| Sample_geo_accession | GSM757148
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757148/suppl/GSM757148_Suger_16.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757149 | GPL1355 |
|
Maple Syrup, biological rep1
|
rats fed maple syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: maple syrup
|
|
| Sample_geo_accession | GSM757149
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757149/suppl/GSM757149_Medium_3.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757150 | GPL1355 |
|
Maple Syrup, biological rep2
|
rats fed maple syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: maple syrup
|
|
| Sample_geo_accession | GSM757150
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757150/suppl/GSM757150_Medium_7.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757151 | GPL1355 |
|
Maple Syrup, biological rep3
|
rats fed maple syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: maple syrup
|
|
| Sample_geo_accession | GSM757151
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757151/suppl/GSM757151_Medium_9.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757152 | GPL1355 |
|
Maple Syrup, biological rep4
|
rats fed maple syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: maple syrup
|
|
| Sample_geo_accession | GSM757152
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757152/suppl/GSM757152_Medium_11.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757153 | GPL1355 |
|
Maple Syrup, biological rep5
|
rats fed maple syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: maple syrup
|
|
| Sample_geo_accession | GSM757153
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757153/suppl/GSM757153_Medium_13.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
GSM757154 | GPL1355 |
|
Maple Syrup, biological rep6
|
rats fed maple syrup diet for 11days
|
strain: Wistar
gender: male
tissue: liver
age: five-week-old
treatment: maple syrup
|
|
| Sample_geo_accession | GSM757154
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Jul 08 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Livers were excised and subsequently immersed in RNAlater (Applied Biosystems Japan, Tokyo, Japan) at 4˚C overnight.
| | Sample_growth_protocol_ch1 = Rats were quarantined and conditioned by administration of the authentic AIN93G diet for 4 days. Rats had free access to the diet and drinking water during this preliminary feeding. For feeding tests, they were dichotomized (n | 7 and 8) for maple syrup and sugar mix syrup group, respectively, and then fed for 11 days on either the AIN93G diet containing 20% maple syrup or on the 20% sugar mix syrup with a similar sugar composition; the amount of maple syrup or the sugar mix syrup was arranged. Rats in both diet groups were fasted for 16 hours, prior to being anesthetically sacrificed for dissection. Rats were housed in a room maintained at 24 ± 1˚C and 48 ± 4% humidity with an artificial lighting, 12 hours/day (8:00-20:00).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA), then purified with an RNeasy mini kit (Qiagen, Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The Affymetrix AGCC system was used to reduce the array images to the intensity values for each probe. These values were then stored in Affymatrix CEL format files and quantified with using the Distribution Free Weighted method(DFW) the statistical language R (http://www.r-project.org/), version 2.7.1.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Asuka,,Kamei
| | Sample_contact_email | fp-kamei@newkast.or.jp
| | Sample_contact_phone | +81-44-280-2187
| | Sample_contact_department | Project on Health and Anti-aging
| | Sample_contact_institute | Kanagawa Academy of Science and technology
| | Sample_contact_address | LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi
| | Sample_contact_city | Kanagawa
| | Sample_contact_zip/postal_code | 210-0821
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM757nnn/GSM757154/suppl/GSM757154_Medium_17.CEL.gz
| | Sample_series_id | GSE30532
| | Sample_data_row_count | 31099
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
