Sample_geo_accession | GSM758693
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Sample_status | Public on Jul 12 2011
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Sample_submission_date | Jul 12 2011
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Sample_last_update_date | Jul 12 2011
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_treatment_protocol_ch1 | injection of LPS 10 mg/kg intraperitoneally at t=0hr followed by 0.9% saline 10 ml/kg injected intraperitoneally at t=18hr
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Sample_growth_protocol_ch1 | Standard light-dark cycle and standard ad libitum diet prior to and during experiment
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HT_MG-430A 24 A and B Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Array Station.
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Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip HT Array Plate Scanner
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Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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Sample_platform_id | GPL8760
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Sample_contact_name | Samir,,Parikh
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Sample_contact_institute | Beth Israel Deaconess Medical Center
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Sample_contact_address | 330 Brookline Avenue, RN280C
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Sample_contact_city | Boston
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Sample_contact_zip/postal_code | 02215
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Sample_contact_country | USA
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM758nnn/GSM758693/suppl/GSM758693.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM758nnn/GSM758693/suppl/GSM758693.CHP.gz
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Sample_series_id | GSE30576
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Sample_data_row_count | 22601
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