Search results for the GEO ID: GSE30643  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM759883 | GPL570 |
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Parietal lobe of G114V gCJD
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Human brain from a G114V gCJD patient(postmortem)
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tissue: Brain parietal lobe
genotype: PRNP-G114V
age: 47 year
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Gene expression data from parietal cortex of G114V gCJD patient brain
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| Sample_geo_accession | GSM759883
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA of parietal cortex of the patient was extracted with RNeasy Mini Kit (Qiagen), according to the manufacturer’s instruction. Total RNA of control was purchased from Clontech.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Label protocol: Biotinylated cRNA were prepared according to the Manual of MessageAmp Premier RNA Amplification Kit from 200ng total RNA (Cat. AM1792, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 with constant rotation at the speed of 60 rpm. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix scanners 3000 7G.
| | Sample_data_processing | The CEL image was processed using the Affymetrix GCOS 1.4 software. The generated documents were analyzed according to the “Affymetrix Statistical Algorithms Description Document”. The ontologies (GO) and molecular functions of the genes from the patient that showed 6-fold increased or decreased expression compared with that of normal control were further analyzed by CapitalBio® Molecule Annotation System V3.0 (MAS3.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | chan,,tian
| | Sample_contact_email | tianchan_cdc@126.com
| | Sample_contact_phone | 861058900818
| | Sample_contact_institute | Chinese Center for Disease Control and Prevention, China CDC
| | Sample_contact_address | Changbai Road, Changping district
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 102206
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759883/suppl/GSM759883.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759883/suppl/GSM759883.CHP.gz
| | Sample_series_id | GSE30643
| | Sample_data_row_count | 54675
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GSM759884 | GPL570 |
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Parietal lobe of control
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Commercial normal human brain total RNA(clontech)
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tissue: Brain parietal lobe
genotype: PRNP-Wild type
age: 35-89 year
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Gene expression data from commercial normal human parietal cortex pooled from four male/female
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| Sample_geo_accession | GSM759884
| | Sample_status | Public on Jul 01 2013
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Jul 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA of control was purchased from Clontech. See manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Label protocol: Biotinylated cRNA were prepared according to the Manual of MessageAmp Premier RNA Amplification Kit from 200ng total RNA (Cat. AM1792, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 with constant rotation at the speed of 60 rpm. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix scanners 3000 7G.
| | Sample_data_processing | The CEL image was processed using the Affymetrix GCOS 1.4 software. The generated documents were analyzed according to the “Affymetrix Statistical Algorithms Description Document”. The ontologies (GO) and molecular functions of the genes from the patient that showed 6-fold increased or decreased expression compared with that of normal control were further analyzed by CapitalBio® Molecule Annotation System V3.0 (MAS3.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | chan,,tian
| | Sample_contact_email | tianchan_cdc@126.com
| | Sample_contact_phone | 861058900818
| | Sample_contact_institute | Chinese Center for Disease Control and Prevention, China CDC
| | Sample_contact_address | Changbai Road, Changping district
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 102206
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759884/suppl/GSM759884.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759884/suppl/GSM759884.CHP.gz
| | Sample_series_id | GSE30643
| | Sample_data_row_count | 54675
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