Search results for the GEO ID: GSE30644  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM759894 | GPL570 |
|
MM1S, untreated, biological rep1
|
untreated MM1S
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
untreated MM1S
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759894
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759894/suppl/GSM759894_RR2009020646.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759895 | GPL570 |
|
MM1S, salmeterol treated, biological rep1
|
MM1S cells treated with 1nM Salmeterol for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 1nM Salmeterol for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759895
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759895/suppl/GSM759895_RR2009020647.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759896 | GPL570 |
|
MM1S, low dose dexomethasone treated, biological rep1
|
MM1S cells treated with25nM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with25nM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759896
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759896/suppl/GSM759896_RR2009020648.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759897 | GPL570 |
|
MM1S, high dose dexomethasone treated, biological rep1
|
MM1S cells treated with 2µM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 2µM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759897
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759897/suppl/GSM759897_RR2009020649.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759898 | GPL570 |
|
MM1S, salmeterol and low dose dexamethasone treated, biological rep1
|
MM1S cells treated with 1nM salmeterol and 25nM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 1nM salmeterol and 25nM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759898
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759898/suppl/GSM759898_RR2009020650.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759899 | GPL570 |
|
MM1S, CGS-21680 treated, biological rep1
|
MM1S cells treated with 12.5nM CGS-21680 for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 12.5nM CGS-21680 for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759899
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759899/suppl/GSM759899_RR2009020651.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759900 | GPL570 |
|
MM1S, CGS-21680 and low dose dexamethasone treated, biological rep1
|
MM1S cells treated with 12.5nM CGS-21680 and 25nM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 12.5nM CGS-21680 and 25nM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759900
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759900/suppl/GSM759900_RR2009020652.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759901 | GPL570 |
|
MM1S, untreated, biological rep2
|
untreated MM1S
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
untreated MM1S
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759901
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759901/suppl/GSM759901_RR2009020653.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759902 | GPL570 |
|
MM1S, salmeterol treated, biological rep2
|
MM1S cells treated with 1nM Salmeterol for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 1nM Salmeterol for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759902
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759902/suppl/GSM759902_RR2009020654.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759903 | GPL570 |
|
MM1S, low dose dexomethasone treated, biological rep2
|
MM1S cells treated with25nM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with25nM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759903
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759903/suppl/GSM759903_RR2009020655.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759904 | GPL570 |
|
MM1S, high dose dexomethasone treated, biological rep2
|
MM1S cells treated with 2µM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 2µM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759904
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759904/suppl/GSM759904_RR2009020656.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759905 | GPL570 |
|
MM1S, salmeterol and low dose dexamethasone treated, biological rep2
|
MM1S cells treated with 1nM salmeterol and 25nM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 1nM salmeterol and 25nM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759905
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759905/suppl/GSM759905_RR2009020657.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759906 | GPL570 |
|
MM1S, CGS-21680 treated, biological rep2
|
MM1S cells treated with 12.5nM CGS-21680 for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 12.5nM CGS-21680 for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759906
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759906/suppl/GSM759906_RR2009020658.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
|
GSM759907 | GPL570 |
|
MM1S, CGS-21680 and low dose dexamethasone treated, biological rep2
|
MM1S cells treated with 12.5nM CGS-21680 and 25nM dexamethasone for 6 hours
|
cell line: MM1S
cell type: Multiple myeloma cell line
|
MM1S cells treated with 12.5nM CGS-21680 and 25nM dexamethasone for 6 hours
Greenstein S et al., Exp Hematol 31:271
|
| Sample_geo_accession | GSM759907
| | Sample_status | Public on Apr 01 2012
| | Sample_submission_date | Jul 13 2011
| | Sample_last_update_date | Apr 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM1S cells were treated with the compounds as indicated for 6 hours prior to the RNA isolation
| | Sample_growth_protocol_ch1 | MM1S cells were maintained in RPMI-1640 +10% Fetal bovine serum at 37oC, 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen miniprep kit according to the manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containign biotinylated CTP ad UTP in addition to the four unmodified ribonucleoside triphosphates.
| | Sample_hyb_protocol | The fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a mircroarray chip overnight at 45oC. The chips are transferred to a fluidics intrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated stretavidin (SAPE); additonal fluors are then added using biotinlyated anti-streptavidin antibody and additional SAPE. The fluidics station used is GeneChip Fluidics Station 450
| | Sample_scan_protocol | The scanner used is Affymetrix GeneChip Scanner 3000, 7G
| | Sample_data_processing | The data were analyzed with Gene Pattern module ExpressionFileCreater using GCRMA method, quantile normalization, background correction and median scaling.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Winnie,F,Tam
| | Sample_contact_email | wtam@zalicus.com
| | Sample_contact_department | Oncology
| | Sample_contact_institute | Zalicus, Inc
| | Sample_contact_address | 245 First Street
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM759nnn/GSM759907/suppl/GSM759907_RR2009020659.CEL.gz
| | Sample_series_id | GSE30644
| | Sample_data_row_count | 54675
| | |
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