Search results for the GEO ID: GSE30745  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM762917 | GPL1261 |
|
shMyb.2572 biol repl 1, 3 d dox, sorted
|
TRMPV-Neo.shMyb.2572 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: shMyb.2572
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762917
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762917/suppl/GSM762917_MYB25-1.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762918 | GPL1261 |
|
shMyb.2572 biol repl 2, 3 d dox, sorted
|
TRMPV-Neo.shMyb.2572 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: shMyb.2572
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762918
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762918/suppl/GSM762918_MYB25-2.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762919 | GPL1261 |
|
shMyb.2572 biol repl 3, 3 d dox, sorted
|
TRMPV-Neo.shMyb.2572 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: shMyb.2572
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762919
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762919/suppl/GSM762919_MYB25-3.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762920 | GPL1261 |
|
shMyb.2652 biol repl 1, 3 d dox, sorted
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TRMPV-Neo.shMyb.2652 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: shMyb.2652
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762920
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762920/suppl/GSM762920_MYB26-1.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762921 | GPL1261 |
|
shMyb.2652 biol repl 2, 3 d dox, sorted
|
TRMPV-Neo.shMyb.2652 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: shMyb.2652
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762921
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762921/suppl/GSM762921_MYB26-2.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762922 | GPL1261 |
|
shMyb.2652 biol repl 3, 3 d dox, sorted
|
TRMPV-Neo.shMyb.2652 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: shMyb.2652
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762922
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762922/suppl/GSM762922_MYB26-3.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762923 | GPL1261 |
|
Rec vector biol repl 1, 3 d dox, sorted
|
TRMPV-Neo.Rec vector expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: empty vector
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762923
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762923/suppl/GSM762923_REC-1.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762924 | GPL1261 |
|
Rec vector biol repl 2, 3 d dox, sorted
|
TRMPV-Neo.Rec vector expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: empty vector
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762924
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762924/suppl/GSM762924_REC-2.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762925 | GPL1261 |
|
Rec vector biol repl 3, 3 d dox, sorted
|
TRMPV-Neo.Rec vector expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: empty vector
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762925
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762925/suppl/GSM762925_REC-3.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762926 | GPL1261 |
|
shRen.713 biol repl 1, 3 d dox, sorted
|
TRMPV-Neo.shRen.713 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: luciferase
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762926
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762926/suppl/GSM762926_REN-1.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762927 | GPL1261 |
|
shRen.713 biol repl 2, 3 d dox, sorted
|
TRMPV-Neo.shRen.713 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: luciferase
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762927
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762927/suppl/GSM762927_REN-2.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
|
GSM762928 | GPL1261 |
|
shRen.713 biol repl 3, 3 d dox, sorted
|
TRMPV-Neo.shRen.713 expressing MLL-AF9/NrasG12D AML cells, 3 d dox, sorted
|
cell type: murine AML cell line
knockdown: luciferase
genetic background: C57BL\6
|
|
| Sample_geo_accession | GSM762928
| | Sample_status | Public on Aug 05 2011
| | Sample_submission_date | Jul 18 2011
| | Sample_last_update_date | Aug 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | shRNA expression was induced by doxycycline treatment (1.0 ug/mL for 3 days).
| | Sample_growth_protocol_ch1 | Cells were cultured in RPMI1640 + 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturers instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Samples were Amplified by a Modified T7 IVT, with Ambion's Message Amp II Biotin Enhanced kit according to Manufacturer's instruction.
| | Sample_hyb_protocol | Samples were hybridized using the Affymetrix Hybridization, Wash and Scan kit (P/N 900720) according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2), using fluidics protocol FS450_0004 in a GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Command Console Software on a 3000 7G Affymetrix GeneChip Scanner (P/N GCS 3000)
| | Sample_data_processing | Raw data were processed in two ways: (1) A probeset-based analysis was performed using the RMA algorithm in GenePattern. (2) A gene-based analysis was performed by processing raw data using FARMS method (Hochreiter et al., 2006) with an up-to-date probe set definition based on Entrez gene sequences (Dai et al. 2005).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Johannes,,Zuber
| | Sample_contact_email | zuber@imp.ac.at
| | Sample_contact_phone | ++43-1-79730-3410
| | Sample_contact_laboratory | Zuber Lab
| | Sample_contact_institute | Research Institute for Molecular Pathology (IMP)
| | Sample_contact_address | Dr. Bohr-Gasse 7
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_contact_web_link | http://www.imp.ac.at/research/johannes-zuber
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM762nnn/GSM762928/suppl/GSM762928_REN-3.CEL.gz
| | Sample_series_id | GSE30745
| | Sample_series_id | GSE30747
| | Sample_data_row_count | 45101
| | |
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