Search results for the GEO ID: GSE30767 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM763494 | GPL1261 |
|
E9.5 wildtype ms. Forebrain, rep 1
|
E9.5 wildtype ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: wild type
age: E9.5
|
|
Sample_geo_accession | GSM763494
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763494/suppl/GSM763494_DDA001-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763494/suppl/GSM763494_DDA001-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763495 | GPL1261 |
|
E9.5 wildtype ms. Forebrain, rep 2
|
E9.5 wildtype ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: wild type
age: E9.5
|
|
Sample_geo_accession | GSM763495
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763495/suppl/GSM763495_DDA003-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763495/suppl/GSM763495_DDA003-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763496 | GPL1261 |
|
E9.5 wildtype ms. Forebrain, rep 3
|
E9.5 wildtype ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: wild type
age: E9.5
|
|
Sample_geo_accession | GSM763496
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763496/suppl/GSM763496_DDA004-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763496/suppl/GSM763496_DDA004-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763497 | GPL1261 |
|
E9.5 wildtype ms. Forebrain, rep 4
|
E9.5 wildtype ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: wild type
age: E9.5
|
|
Sample_geo_accession | GSM763497
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763497/suppl/GSM763497_DDA005-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763497/suppl/GSM763497_DDA005-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763498 | GPL1261 |
|
E9.5 VEGF120 ms. Forebrain, rep 1
|
E9.5 VEGF120 ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: knock-in cDNA with the VEGF120 isoform of the VEGF-A gene, VEGF164 deficient
age: E9.5
|
|
Sample_geo_accession | GSM763498
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763498/suppl/GSM763498_DDB001-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763498/suppl/GSM763498_DDB001-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763499 | GPL1261 |
|
E9.5 VEGF120 ms. Forebrain, rep 2
|
E9.5 VEGF120 ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: knock-in cDNA with the VEGF120 isoform of the VEGF-A gene, VEGF164 deficient
age: E9.5
|
|
Sample_geo_accession | GSM763499
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763499/suppl/GSM763499_DDB002-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763499/suppl/GSM763499_DDB002-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763500 | GPL1261 |
|
E9.5 VEGF120 ms. Forebrain, rep 3
|
E9.5 VEGF120 ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: knock-in cDNA with the VEGF120 isoform of the VEGF-A gene, VEGF164 deficient
age: E9.5
|
|
Sample_geo_accession | GSM763500
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763500/suppl/GSM763500_DDB003-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763500/suppl/GSM763500_DDB003-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
GSM763501 | GPL1261 |
|
E9.5 VEGF120 ms. Forebrain, rep 4
|
E9.5 VEGF120 ms. Forebrain
|
genetic background: C57Bl/6
tissue: forebrain
genotype: knock-in cDNA with the VEGF120 isoform of the VEGF-A gene, VEGF164 deficient
age: E9.5
|
|
Sample_geo_accession | GSM763501
| Sample_status | Public on Dec 01 2011
| Sample_submission_date | Jul 19 2011
| Sample_last_update_date | Dec 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Embryonic timed pregnancies were collected at E9.5 and the forebrain microdissected. The VEGF120 mice express only the VEGF120 isoform and lack the other isoforms of the VEGF-A gene.
| Sample_growth_protocol_ch1 | The mice were generated by knock-in cDNA with the VEGF120 isoform of the VEGF-A gene [Carmeliet et al., Nature Medicine 1999; 5(5):495-502].
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using PicoPure™ RNA isolation kit (Applied Biosystems), according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by a modified Eberwine RT-IVT procedure using the 3' IVT Express kit (Affymetrix), according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip 430.2 arrays (Affymetrix; catalog no. 901230). GeneChips were washed and stained with streptavidin-phycoerythrin using the Affymetrix Fluidics Station 450, according to the manufacturer's recommended protocol.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000 7G.
| Sample_data_processing | The data were analyzed with AGCC and MAS 5.0 in Expression Console using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity (TGT) of each array was set to 250.
| Sample_platform_id | GPL1261
| Sample_contact_name | Diane,Catherine,Darland
| Sample_contact_email | diane.darland@und.nodak.edu
| Sample_contact_phone | 701-777-4597
| Sample_contact_fax | 701-777-2623
| Sample_contact_department | Department of Biology
| Sample_contact_institute | University of North Dakota
| Sample_contact_address | 10 Cornell Street/Campus Drive
| Sample_contact_city | Grand Forks
| Sample_contact_state | ND
| Sample_contact_zip/postal_code | 58202-9019
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763501/suppl/GSM763501_DDB004-430_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763501/suppl/GSM763501_DDB004-430_2.CHP.gz
| Sample_series_id | GSE30767
| Sample_data_row_count | 45101
| |
|
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