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Search results for the GEO ID: GSE3077
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Description
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GSM66935
GPL570
1_100US_HG_U133_Plus_2 Ficol separated PBMC Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 9_100US_HG_U133_Plus_2 (GSM66936)
GSM66936
GPL570
9_100US_HG_U133_Plus_2 PBMC Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 1_US_100_HG_U133_Plus_2 (GSM66935).
GSM66937
GPL570
10_95US_5Placenta_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 2_95US_5Placenta_HG_U133_Plus_2 (GSM66938).
GSM66938
GPL570
2_95US_5Placenta_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 10_95US_5Placenta_HG_U133_Plus_2 (GSM66937)
GSM66939
GPL570
3_75US_25Placenta_HG_133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 11_75US_25Placenta_HG_U133_Plus_2 (GSM66940)
GSM66940
GPL570
11_75US_25Placenta_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 3_75US_25Placenta_HG_U133_Plus_2 (GSM66939)
GSM66941
GPL570
4_50US_50Placenta_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 12_50US_50Placenta_HG_U133_Plus_2 (GSM66942)
GSM66942
GPL570
12_50US_50Placenta_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 4_50US_50Placenta_HG_U133_Plus_2 (GSM66941)
GSM66943
GPL570
5_25US_75Placenta_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 13_25US_75Placenta_HG_U133_Plus_2 (GSM66944)
GSM66944
GPL570
13_25US_75Placenta_2_HG_U133_Plus_2 PBMC and Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 5_25US_75Placenta_HG_U133_Plus_2 (GSM66945)
GSM66945
GPL570
14_Placenta_100_HG_U133_Plus_2 Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 6_Placenta_100_HG_U133_Plus_2 (GSM66946)
GSM66946
GPL570
6_Placenta_100_HG_U133_Plus_2 Placenta Approximately 50 mL whole blood was collected from 30 adult, apparently healthy, volunteers using Acid Citrate Dextrose (ACD) as an anti-coagulant. Samples were processed immediately following blood draw. Cells were isolated by Ficoll gradient centrifugation and total RNA was isolated using Trizol (Invitrogen Life Technologies; Carlsbad, CA) according to manufacturer’s protocol, aliquoted and stored at -80°C until use. Whole placenta was collected and immediately frozen in liquid nitrogen. RNA was extracted using TRI reagent, purified using RNeasy columns (Qiagen; Valencia, CA), aliquoted and stored at -80°C until use. RNA mixtures (100:0, 95:5, 75:25, 50:50, 25:75, 0:100; PBMC: placenta) were prepared in single aliquots. Technical labeling replicate of 14_Placenta_100_HG_U133_Plus_2 (GSM66945)
 
 
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