Search results for the GEO ID: GSE30781  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM763651 | GPL570 |
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THCEH (cyclin)
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Transduced human corneal endothelial cells
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tissue: Cornea
cell type: corneal endothelial
genotype/variation: transduced by Cdk4R24C/CyclinD1
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Gene expression data from human corneal endothelial cells transduced by Cdk4R24C/CyclinD1
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| Sample_geo_accession | GSM763651
| | Sample_status | Public on Oct 30 2011
| | Sample_submission_date | Jul 19 2011
| | Sample_last_update_date | Oct 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Lentiviral vector plasmids, CSII-CMV-cyclin D1 and -CDK4R24C were constructed by recombination using the Gateway system (Invitrogen, Carlsbad, CA).The recombinant retrovirus vector plasmids, pCLXSN-16E6E7 encoding HPV16 E6/E7 (16E6E7) was produced. Stably transduced cells with an expanded life span were designated transduced human corneal endothelial cell by E6/E7 (THCEC (E6/E7) ) and transduced human corneal endothelial cell by Cdk4R24C/cyclinD1 (THCEH (Cyclin) ).
| | Sample_growth_protocol_ch1 | The corneal piece, which was grossly normal with no pathological lesions, was cut 1.5 mm from the corneal limbus, avoiding contamination of the trabecular meshwork tissue. HCEC with Descemet's membrane were stripped from the posterior surface of the corneal tissue with sterile surgical forceps under a dissecting microscope. They were cut into 2 pieces and cultured in a cell culture dish covered with Type IV collagen in a growth medium (GM); Dulbecco's modified Eagle's medium (DMEM)/Nutrient mixture F12 (1:1) with high glucose supplemented with 10% fetal bovine serum, insulin-transferrin-selenium and MEM-NEAA (Gibco, Auckland, NZ).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 2 x 10E6 cultured HCEC using the RNeasy Plus mini-kitH (Qiagen, Germantown/Gaithersburg, MA) according to the manufacturer's instructions and quantified by absorption at 260 nm.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labelled.
| | Sample_hyb_protocol | Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akihiro,,Umezawa
| | Sample_contact_email | umezawa@1985.jukuin.keio.ac.jp
| | Sample_contact_phone | 81-3-5494-7047
| | Sample_contact_fax | 81-3-5494-7048
| | Sample_contact_department | Reproductive Biology
| | Sample_contact_institute | National Center for Child Health and Development
| | Sample_contact_address | 2-10-1 Okura
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://1985.jukuin.keio.ac.jp/umezawa/HTML
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763651/suppl/GSM763651_EY1409CorEnd_5.CEL.gz
| | Sample_series_id | GSE30781
| | Sample_data_row_count | 54675
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GSM763652 | GPL570 |
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THCEC (E6/E7)
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Transduced human corneal endothelial cells
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tissue: Cornea
cell type: corneal endothelial
genotype/variation: transduced by human papillomavirus type E6/E7
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Gene expression data from human corneal endothelial cells transduced by human papillomavirus type E6/E7
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| Sample_geo_accession | GSM763652
| | Sample_status | Public on Oct 30 2011
| | Sample_submission_date | Jul 19 2011
| | Sample_last_update_date | Oct 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Lentiviral vector plasmids, CSII-CMV-cyclin D1 and -CDK4R24C were constructed by recombination using the Gateway system (Invitrogen, Carlsbad, CA).The recombinant retrovirus vector plasmids, pCLXSN-16E6E7 encoding HPV16 E6/E7 (16E6E7) was produced. Stably transduced cells with an expanded life span were designated transduced human corneal endothelial cell by E6/E7 (THCEC (E6/E7) ) and transduced human corneal endothelial cell by Cdk4R24C/cyclinD1 (THCEH (Cyclin) ).
| | Sample_growth_protocol_ch1 | The corneal piece, which was grossly normal with no pathological lesions, was cut 1.5 mm from the corneal limbus, avoiding contamination of the trabecular meshwork tissue. HCEC with Descemet's membrane were stripped from the posterior surface of the corneal tissue with sterile surgical forceps under a dissecting microscope. They were cut into 2 pieces and cultured in a cell culture dish covered with Type IV collagen in a growth medium (GM); Dulbecco's modified Eagle's medium (DMEM)/Nutrient mixture F12 (1:1) with high glucose supplemented with 10% fetal bovine serum, insulin-transferrin-selenium and MEM-NEAA (Gibco, Auckland, NZ).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 2 x 10E6 cultured HCEC using the RNeasy Plus mini-kitH (Qiagen, Germantown/Gaithersburg, MA) according to the manufacturer's instructions and quantified by absorption at 260 nm.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labelled.
| | Sample_hyb_protocol | Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akihiro,,Umezawa
| | Sample_contact_email | umezawa@1985.jukuin.keio.ac.jp
| | Sample_contact_phone | 81-3-5494-7047
| | Sample_contact_fax | 81-3-5494-7048
| | Sample_contact_department | Reproductive Biology
| | Sample_contact_institute | National Center for Child Health and Development
| | Sample_contact_address | 2-10-1 Okura
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://1985.jukuin.keio.ac.jp/umezawa/HTML
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763652/suppl/GSM763652_EY1409CorEnd_2.CEL.gz
| | Sample_series_id | GSE30781
| | Sample_data_row_count | 54675
| | |
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GSM763653 | GPL570 |
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HCEC
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Primary human corneal endothelial cells
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tissue: Cornea
cell type: corneal endothelial
genotype/variation: non-transduced (primary)
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Gene expression data from primary human corneal endothelial cells
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| Sample_geo_accession | GSM763653
| | Sample_status | Public on Oct 30 2011
| | Sample_submission_date | Jul 19 2011
| | Sample_last_update_date | Oct 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Lentiviral vector plasmids, CSII-CMV-cyclin D1 and -CDK4R24C were constructed by recombination using the Gateway system (Invitrogen, Carlsbad, CA).The recombinant retrovirus vector plasmids, pCLXSN-16E6E7 encoding HPV16 E6/E7 (16E6E7) was produced. Stably transduced cells with an expanded life span were designated transduced human corneal endothelial cell by E6/E7 (THCEC (E6/E7) ) and transduced human corneal endothelial cell by Cdk4R24C/cyclinD1 (THCEH (Cyclin) ).
| | Sample_growth_protocol_ch1 | The corneal piece, which was grossly normal with no pathological lesions, was cut 1.5 mm from the corneal limbus, avoiding contamination of the trabecular meshwork tissue. HCEC with Descemet's membrane were stripped from the posterior surface of the corneal tissue with sterile surgical forceps under a dissecting microscope. They were cut into 2 pieces and cultured in a cell culture dish covered with Type IV collagen in a growth medium (GM); Dulbecco's modified Eagle's medium (DMEM)/Nutrient mixture F12 (1:1) with high glucose supplemented with 10% fetal bovine serum, insulin-transferrin-selenium and MEM-NEAA (Gibco, Auckland, NZ).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from 2 x 10E6 cultured HCEC using the RNeasy Plus mini-kitH (Qiagen, Germantown/Gaithersburg, MA) according to the manufacturer's instructions and quantified by absorption at 260 nm.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labelled.
| | Sample_hyb_protocol | Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Akihiro,,Umezawa
| | Sample_contact_email | umezawa@1985.jukuin.keio.ac.jp
| | Sample_contact_phone | 81-3-5494-7047
| | Sample_contact_fax | 81-3-5494-7048
| | Sample_contact_department | Reproductive Biology
| | Sample_contact_institute | National Center for Child Health and Development
| | Sample_contact_address | 2-10-1 Okura
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 157-8535
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://1985.jukuin.keio.ac.jp/umezawa/HTML
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM763nnn/GSM763653/suppl/GSM763653_EY1409CorEnd_original.CEL.gz
| | Sample_series_id | GSE30781
| | Sample_data_row_count | 54675
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