Result

Search results for the GEO ID: GSE30807

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Title

Source name

Description

Characteristics

GSM764199

GPL570

mesenchymal stem cells, lot#7032R, 2th passage

mesenchymal stem cells, lot# 7032R, 2th passage

cell type: bone marrow mesenchymal stem cells

Gene expression data from bone marrow mesenchymal stem cells.Cells were purchased from TUCGT (Tulane University Center for Gene Therapy) under material transfer agreement. Cells were cultured according to manufacturer's protocol.

Sample_geo_accession	GSM764199
Sample_status	Public on Dec 30 2012
Sample_submission_date	Jul 20 2011
Sample_last_update_date	Dec 30 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were lysed in 600 ul of buffer RLT (QIAGEN).
Sample_growth_protocol_ch1	Cells were cultured according to manufacturer's protocol. Cells were grown at 37°C in a 5% CO2 atmosphere.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-membrane RNeasy spin columns according to manufacturer's protocol (QIAGEN, RNeasy)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45℃ on Affymetrix human genome HG-U133A genechip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Scanned on a GeneArray scanner.
Sample_data_processing	The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000. dChip was used for probe set summarization.
Sample_platform_id	GPL570
Sample_contact_name	Lingtao,,Wu
Sample_contact_email	lingtaow@usc.edu
Sample_contact_phone	323-361-6318
Sample_contact_fax	323-361-3669
Sample_contact_department	Children hispotial of Los angeles, Dept. of Pathology, MS# 103
Sample_contact_institute	University of Southern California
Sample_contact_address	4650 Sunset Blvd.
Sample_contact_city	Los Angeles
Sample_contact_state	CA
Sample_contact_zip/postal_code	90027
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM764nnn/GSM764199/suppl/GSM764199.CEL.gz
Sample_series_id	GSE30807
Sample_data_row_count	54675

GSM764200

GPL570

osteosarcoma U2OS cells

The total RNA from osteosarcoma cell line U2OS

cell type: osteosarcoma U2OS cells

Gene expression data from human osteosarcoma U2OS cells. Cells were maintained in RPMI 1640 (Gibco, 2 g/l glucose) with 10% fetal calf serum (NCS, Gibco) plus 2 mmol/l glutamine and 50 U/ml penicillin. Organ: bone; Disease: osteosarcoma; Age: 15 years; Gender: female; Comments: J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Sample_geo_accession	GSM764200
Sample_status	Public on Dec 30 2012
Sample_submission_date	Jul 20 2011
Sample_last_update_date	Dec 30 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were lysed in 600 ul of buffer RLT (QIAGEN).
Sample_growth_protocol_ch1	Cells were cultured according to manufacturer's protocol. Cells were grown at 37°C in a 5% CO2 atmosphere.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-membrane RNeasy spin columns according to manufacturer's protocol (QIAGEN, RNeasy)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45℃ on Affymetrix human genome HG-U133A genechip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Scanned on a GeneArray scanner.
Sample_data_processing	The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000. dChip was used for probe set summarization.
Sample_platform_id	GPL570
Sample_contact_name	Lingtao,,Wu
Sample_contact_email	lingtaow@usc.edu
Sample_contact_phone	323-361-6318
Sample_contact_fax	323-361-3669
Sample_contact_department	Children hispotial of Los angeles, Dept. of Pathology, MS# 103
Sample_contact_institute	University of Southern California
Sample_contact_address	4650 Sunset Blvd.
Sample_contact_city	Los Angeles
Sample_contact_state	CA
Sample_contact_zip/postal_code	90027
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM764nnn/GSM764200/suppl/GSM764200.CEL.gz
Sample_series_id	GSE30807
Sample_data_row_count	54675

GSM764201

GPL570

osteosarcoma U2OS derived cell line UT2

The total RNA from osteosarcoma U2OS derived cell line-UT2

cell type: osteosarcoma U2OS derived cell line UT2

Gene expression data from human osteosarcoma UT2 cells.Cells were maintained in RPMI 1640 (Gibco, 2 g/l glucose) with 10% fetal calf serum (NCS, Gibco) plus 2 mmol/l glutamine and 50 U/ml penicillin. Derived from the second human–mouse xenotransplantation of U2OS cell-formed osteosarcoma tissues.

Sample_geo_accession	GSM764201
Sample_status	Public on Dec 30 2012
Sample_submission_date	Jul 20 2011
Sample_last_update_date	Dec 30 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were lysed in 600 ul of buffer RLT (QIAGEN).
Sample_growth_protocol_ch1	Cells were cultured according to manufacturer's protocol. Cells were grown at 37°C in a 5% CO2 atmosphere.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-membrane RNeasy spin columns according to manufacturer's protocol (QIAGEN, RNeasy)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45℃ on Affymetrix human genome HG-U133A genechip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Scanned on a GeneArray scanner.
Sample_data_processing	The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000. dChip was used for probe set summarization.
Sample_platform_id	GPL570
Sample_contact_name	Lingtao,,Wu
Sample_contact_email	lingtaow@usc.edu
Sample_contact_phone	323-361-6318
Sample_contact_fax	323-361-3669
Sample_contact_department	Children hispotial of Los angeles, Dept. of Pathology, MS# 103
Sample_contact_institute	University of Southern California
Sample_contact_address	4650 Sunset Blvd.
Sample_contact_city	Los Angeles
Sample_contact_state	CA
Sample_contact_zip/postal_code	90027
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM764nnn/GSM764201/suppl/GSM764201.CEL.gz
Sample_series_id	GSE30807
Sample_data_row_count	54675

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