Result

Search results for the GEO ID: GSE30834

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM765953

GPL1261

3T3RasV12, replicate 1

3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12

RasV12_rep1

Sample_geo_accession	GSM765953
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765953/suppl/GSM765953.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765954

GPL1261

3T3RasV12, replicate 2

3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV13

RasV12_rep2

Sample_geo_accession	GSM765954
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765954/suppl/GSM765954.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765955

GPL1261

3T3RasV12, replicate 3

3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV14

RasV12_rep3

Sample_geo_accession	GSM765955
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765955/suppl/GSM765955.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765956

GPL1261

C/EBPβ + 3T3RasV12, replicate 1

C/EBPβ + 3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12 and C/EBPβ

RasV12+CebpB_rep1

Sample_geo_accession	GSM765956
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765956/suppl/GSM765956.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765957

GPL1261

C/EBPβ + 3T3RasV12, replicate 2

C/EBPβ + 3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12 and C/EBPβ

RasV12+CebpB_rep2

Sample_geo_accession	GSM765957
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765957/suppl/GSM765957.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765958

GPL1261

C/EBPβ + 3T3RasV12, replicate 3

C/EBPβ + 3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12 and C/EBPβ

RasV12+CebpB_rep3

Sample_geo_accession	GSM765958
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765958/suppl/GSM765958.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765959

GPL1261

C/EBPβ-UTR + 3T3RasV12, replicate 1

C/EBPβ-UTR + 3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12 and C/EBPβ-UTR

RasV12+CebpB_UTR_rep1

Sample_geo_accession	GSM765959
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765959/suppl/GSM765959.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765960

GPL1261

C/EBPβ-UTR + 3T3RasV12, replicate 2

C/EBPβ-UTR + 3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12 and C/EBPβ-UTR

RasV12+CebpB_UTR_rep2

Sample_geo_accession	GSM765960
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765960/suppl/GSM765960.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

GSM765961

GPL1261

C/EBPβ-UTR + 3T3RasV12, replicate 3

C/EBPβ-UTR + 3T3RasV12

cell line: NIH-3T3 genotype/variation: infected with retroviruses expressing H-RasV12 and C/EBPβ-UTR

RasV12+CebpB_UTR_rep3

Sample_geo_accession	GSM765961
Sample_status	Public on Jul 23 2011
Sample_submission_date	Jul 22 2011
Sample_last_update_date	Jul 23 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were sequentially infected with retroviruses expressing H-RasV12 and C/EBPβ or C/EBPβ-UTR and selected using puromycin and hygromycin. After selection, cells were plated at equivalent densities and harvested 48-72 hours later.
Sample_growth_protocol_ch1	NIH-3T3 cells were purchased from ATCC and passaged in DMEM supplemented with 10% FCS and pen/strep.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from 3T3 cells using Rneasy Kits (Qiagen) according to manufacturer's instructions
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled using Affymetrix One Cycle Target Labeling kit following manufacturer’s protocol
Sample_hyb_protocol	Three replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
Sample_scan_protocol	GeneChips were scanned on Affymetrix GeneChip scanner 3000
Sample_data_processing	Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
Sample_platform_id	GPL1261
Sample_contact_name	Peter,F,Johnson
Sample_contact_email	johnsope@mail.nih.gov
Sample_contact_phone	301 846-1627
Sample_contact_fax	301 846-5991
Sample_contact_laboratory	Laboratory of Cancer Prevention
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	NCI
Sample_contact_address	Bldg 539, rm 122, 1050 Boyles St., NCI-Frederick
Sample_contact_city	Frederick
Sample_contact_state	MD
Sample_contact_zip/postal_code	21702
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM765nnn/GSM765961/suppl/GSM765961.CEL.gz
Sample_series_id	GSE30834
Sample_data_row_count	45101

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