Result

Search results for the GEO ID: GSE30964

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM767687

GPL1355

beta cells, FACS-purified from rats fed ad libitum, biological replicate 1

FACS-purified beta cells from rats fed ad libitum

strain: Wistar gender: male age: 10 weeks cell type: freshly isolated FACS-purified pancreatic beta cells in vivo fed/fasted state: ad libitum feeding

Control1 each biological replicate represents independent isolation from a pool of 5 rats

Sample_geo_accession	GSM767687
Sample_status	Public on Sep 01 2011
Sample_submission_date	Jul 26 2011
Sample_last_update_date	Sep 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Rat beta (92 +- 3% insulin+) were FACS-purified from 10-weeks old Wistar rats and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff	8.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
Sample_scan_protocol	Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
Sample_data_processing	Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction model (PM-MM).
Sample_platform_id	GPL1355
Sample_contact_name	Geert,A.,Martens
Sample_contact_email	geert.martens@vub.ac.be
Sample_contact_department	Diabetes Research Center
Sample_contact_institute	Vrije Universiteit Brussel
Sample_contact_address	Laarbeeklaan 103
Sample_contact_city	Brussels
Sample_contact_zip/postal_code	1090
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767687/suppl/GSM767687_Control1.CEL.gz
Sample_series_id	GSE30964
Sample_data_row_count	31099

GSM767688

GPL1355

beta cells, FACS-purified from rats fed ad libitum, biological replicate 2

FACS-purified beta cells from rats fed ad libitum

strain: Wistar gender: male age: 10 weeks cell type: freshly isolated FACS-purified pancreatic beta cells in vivo fed/fasted state: ad libitum feeding

Control2 each biological replicate represents independent isolation from a pool of 5 rats

Sample_geo_accession	GSM767688
Sample_status	Public on Sep 01 2011
Sample_submission_date	Jul 26 2011
Sample_last_update_date	Sep 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Rat beta (92 +- 3% insulin+) were FACS-purified from 10-weeks old Wistar rats and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff	8.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
Sample_scan_protocol	Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
Sample_data_processing	Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction model (PM-MM).
Sample_platform_id	GPL1355
Sample_contact_name	Geert,A.,Martens
Sample_contact_email	geert.martens@vub.ac.be
Sample_contact_department	Diabetes Research Center
Sample_contact_institute	Vrije Universiteit Brussel
Sample_contact_address	Laarbeeklaan 103
Sample_contact_city	Brussels
Sample_contact_zip/postal_code	1090
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767688/suppl/GSM767688_Control2.CEL.gz
Sample_series_id	GSE30964
Sample_data_row_count	31099

GSM767689

GPL1355

beta cells, FACS-purified from rats fed ad libitum, biological replicate 3

FACS-purified beta cells from rats fed ad libitum

strain: Wistar gender: male age: 10 weeks cell type: freshly isolated FACS-purified pancreatic beta cells in vivo fed/fasted state: ad libitum feeding

Control3 each biological replicate represents independent isolation from a pool of 5 rats

Sample_geo_accession	GSM767689
Sample_status	Public on Sep 01 2011
Sample_submission_date	Jul 26 2011
Sample_last_update_date	Sep 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Rat beta (92 +- 3% insulin+) were FACS-purified from 10-weeks old Wistar rats and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff	8.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
Sample_scan_protocol	Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
Sample_data_processing	Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction model (PM-MM).
Sample_platform_id	GPL1355
Sample_contact_name	Geert,A.,Martens
Sample_contact_email	geert.martens@vub.ac.be
Sample_contact_department	Diabetes Research Center
Sample_contact_institute	Vrije Universiteit Brussel
Sample_contact_address	Laarbeeklaan 103
Sample_contact_city	Brussels
Sample_contact_zip/postal_code	1090
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767689/suppl/GSM767689_Control3.CEL.gz
Sample_series_id	GSE30964
Sample_data_row_count	31099

GSM767690

GPL1355

beta cells, FACS-purified from rats after 24h fast, biological replicate 1

FACS-purified beta cells from rats after 24h fast

strain: Wistar gender: male age: 10 weeks cell type: freshly isolated FACS-purified pancreatic beta cells in vivo fed/fasted state: 24hr fasting

Fast1 each biological replicate represents independent isolation from a pool of 5 rats

Sample_geo_accession	GSM767690
Sample_status	Public on Sep 01 2011
Sample_submission_date	Jul 26 2011
Sample_last_update_date	Sep 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Rat beta (92 +- 3% insulin+) were FACS-purified from 10-weeks old Wistar rats and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff	8.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
Sample_scan_protocol	Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
Sample_data_processing	Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction model (PM-MM).
Sample_platform_id	GPL1355
Sample_contact_name	Geert,A.,Martens
Sample_contact_email	geert.martens@vub.ac.be
Sample_contact_department	Diabetes Research Center
Sample_contact_institute	Vrije Universiteit Brussel
Sample_contact_address	Laarbeeklaan 103
Sample_contact_city	Brussels
Sample_contact_zip/postal_code	1090
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767690/suppl/GSM767690_Fast1.CEL.gz
Sample_series_id	GSE30964
Sample_data_row_count	31099

GSM767691

GPL1355

beta cells, FACS-purified from rats after 24h fast, biological replicate 2

FACS-purified beta cells from rats after 24h fast

strain: Wistar gender: male age: 10 weeks cell type: freshly isolated FACS-purified pancreatic beta cells in vivo fed/fasted state: 24hr fasting

Fast2 each biological replicate represents independent isolation from a pool of 5 rats

Sample_geo_accession	GSM767691
Sample_status	Public on Sep 01 2011
Sample_submission_date	Jul 26 2011
Sample_last_update_date	Sep 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Rat beta (92 +- 3% insulin+) were FACS-purified from 10-weeks old Wistar rats and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff	8.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
Sample_scan_protocol	Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
Sample_data_processing	Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction model (PM-MM).
Sample_platform_id	GPL1355
Sample_contact_name	Geert,A.,Martens
Sample_contact_email	geert.martens@vub.ac.be
Sample_contact_department	Diabetes Research Center
Sample_contact_institute	Vrije Universiteit Brussel
Sample_contact_address	Laarbeeklaan 103
Sample_contact_city	Brussels
Sample_contact_zip/postal_code	1090
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767691/suppl/GSM767691_Fast2.CEL.gz
Sample_series_id	GSE30964
Sample_data_row_count	31099

GSM767692

GPL1355

beta cells, FACS-purified from rats after 24h fast, biological replicate 3

FACS-purified beta cells from rats after 24h fast

strain: Wistar gender: male age: 10 weeks cell type: freshly isolated FACS-purified pancreatic beta cells in vivo fed/fasted state: 24hr fasting

Fast3 each biological replicate represents independent isolation from a pool of 5 rats

Sample_geo_accession	GSM767692
Sample_status	Public on Sep 01 2011
Sample_submission_date	Jul 26 2011
Sample_last_update_date	Sep 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Rat beta (92 +- 3% insulin+) were FACS-purified from 10-weeks old Wistar rats and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1 = Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff	8.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
Sample_scan_protocol	Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
Sample_data_processing	Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction model (PM-MM).
Sample_platform_id	GPL1355
Sample_contact_name	Geert,A.,Martens
Sample_contact_email	geert.martens@vub.ac.be
Sample_contact_department	Diabetes Research Center
Sample_contact_institute	Vrije Universiteit Brussel
Sample_contact_address	Laarbeeklaan 103
Sample_contact_city	Brussels
Sample_contact_zip/postal_code	1090
Sample_contact_country	Belgium
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767692/suppl/GSM767692_Fast3.CEL.gz
Sample_series_id	GSE30964
Sample_data_row_count	31099

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