Search results for the GEO ID: GSE30988  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM767916 | GPL570 |
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HeLa_doxorubicin_24hours
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human cervix carcinoma cell line HeLa_treatment with doxorubicin for 24h
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cell line: HeLa
treatment: doxorubicin for 24h
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| Sample_geo_accession | GSM767916
| | Sample_status | Public on May 23 2012
| | Sample_submission_date | Jul 27 2011
| | Sample_last_update_date | May 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For expression analysis, cells were seeded 24 h before drug treatment in 6-Well plates (300,000 cells per well) and were cultured for the respective time points with doxorubicin, DMSO as solvent respectively.
| | Sample_growth_protocol_ch1 | The human cervix carcinoma cell line HeLa were obtained from the American Type Culture Collection and maintained by culture in RPMI 1640 or DMEM medium supplemented with 10% FCS, 1% glutamine, 50 U/ml penicillin, and 50 µg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA of one batch Doxorubicin treated and untreated (DMSO) HeLa cells for two timepoints (24h, 48h) was extracted using Trizol PeqGold RNA Pure (Peqlab, Erlangen, Germany) according to the manufacturer`s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | T7-RNA polymerase-mediated linear amplification was performed starting with 1µg RNA (One cycle Target labelling protocol; Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA, USA) were employed.
| | Sample_scan_protocol | After hybridization and washing, arrays were scanned using the GeneChip System Confocal Scanner 3000 (Affymetrix)
| | Sample_data_processing | Affymetrix default parameters were used for data extraction (GCOS, MAS5.0). Expression data were transferred to GeneSpring GX version 7.3.1. (Agilent Technologies) and per-chip normalized. Genes which were flagged as present or marginal and exceeding a fold change of 2 in comparison to control were further analysed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sabine,,Ameling
| | Sample_contact_laboratory | Functional Genomics
| | Sample_contact_department | Interfaculty Institute of Genetics and Functional Genomics
| | Sample_contact_institute | Ernst-Moritz-Arndt University of Greifswald
| | Sample_contact_address | Jahnstraße 15a
| | Sample_contact_city | Greifswald
| | Sample_contact_zip/postal_code | 17487
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767916/suppl/GSM767916.CEL.gz
| | Sample_series_id | GSE30988
| | Sample_data_row_count | 54675
| | |
|
GSM767917 | GPL570 |
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HeLa_control_24hours
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human cervix carcinoma cell line HeLa_control
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cell line: HeLa
treatment: none
|
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| Sample_geo_accession | GSM767917
| | Sample_status | Public on May 23 2012
| | Sample_submission_date | Jul 27 2011
| | Sample_last_update_date | May 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For expression analysis, cells were seeded 24 h before drug treatment in 6-Well plates (300,000 cells per well) and were cultured for the respective time points with doxorubicin, DMSO as solvent respectively.
| | Sample_growth_protocol_ch1 | The human cervix carcinoma cell line HeLa were obtained from the American Type Culture Collection and maintained by culture in RPMI 1640 or DMEM medium supplemented with 10% FCS, 1% glutamine, 50 U/ml penicillin, and 50 µg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA of one batch Doxorubicin treated and untreated (DMSO) HeLa cells for two timepoints (24h, 48h) was extracted using Trizol PeqGold RNA Pure (Peqlab, Erlangen, Germany) according to the manufacturer`s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | T7-RNA polymerase-mediated linear amplification was performed starting with 1µg RNA (One cycle Target labelling protocol; Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA, USA) were employed.
| | Sample_scan_protocol | After hybridization and washing, arrays were scanned using the GeneChip System Confocal Scanner 3000 (Affymetrix)
| | Sample_data_processing | Affymetrix default parameters were used for data extraction (GCOS, MAS5.0). Expression data were transferred to GeneSpring GX version 7.3.1. (Agilent Technologies) and per-chip normalized. Genes which were flagged as present or marginal and exceeding a fold change of 2 in comparison to control were further analysed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sabine,,Ameling
| | Sample_contact_laboratory | Functional Genomics
| | Sample_contact_department | Interfaculty Institute of Genetics and Functional Genomics
| | Sample_contact_institute | Ernst-Moritz-Arndt University of Greifswald
| | Sample_contact_address | Jahnstraße 15a
| | Sample_contact_city | Greifswald
| | Sample_contact_zip/postal_code | 17487
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767917/suppl/GSM767917.CEL.gz
| | Sample_series_id | GSE30988
| | Sample_data_row_count | 54675
| | |
|
GSM767918 | GPL570 |
|
HeLa_doxorubicin_48hours
|
human cervix carcinoma cell line HeLa_treatment with doxorubicin for 48h
|
cell line: HeLa
treatment: doxorubicin for 48h
|
|
| Sample_geo_accession | GSM767918
| | Sample_status | Public on May 23 2012
| | Sample_submission_date | Jul 27 2011
| | Sample_last_update_date | May 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For expression analysis, cells were seeded 24 h before drug treatment in 6-Well plates (300,000 cells per well) and were cultured for the respective time points with doxorubicin, DMSO as solvent respectively.
| | Sample_growth_protocol_ch1 | The human cervix carcinoma cell line HeLa were obtained from the American Type Culture Collection and maintained by culture in RPMI 1640 or DMEM medium supplemented with 10% FCS, 1% glutamine, 50 U/ml penicillin, and 50 µg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA of one batch Doxorubicin treated and untreated (DMSO) HeLa cells for two timepoints (24h, 48h) was extracted using Trizol PeqGold RNA Pure (Peqlab, Erlangen, Germany) according to the manufacturer`s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | T7-RNA polymerase-mediated linear amplification was performed starting with 1µg RNA (One cycle Target labelling protocol; Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA, USA) were employed.
| | Sample_scan_protocol | After hybridization and washing, arrays were scanned using the GeneChip System Confocal Scanner 3000 (Affymetrix)
| | Sample_data_processing | Affymetrix default parameters were used for data extraction (GCOS, MAS5.0). Expression data were transferred to GeneSpring GX version 7.3.1. (Agilent Technologies) and per-chip normalized. Genes which were flagged as present or marginal and exceeding a fold change of 2 in comparison to control were further analysed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sabine,,Ameling
| | Sample_contact_laboratory | Functional Genomics
| | Sample_contact_department | Interfaculty Institute of Genetics and Functional Genomics
| | Sample_contact_institute | Ernst-Moritz-Arndt University of Greifswald
| | Sample_contact_address | Jahnstraße 15a
| | Sample_contact_city | Greifswald
| | Sample_contact_zip/postal_code | 17487
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767918/suppl/GSM767918.CEL.gz
| | Sample_series_id | GSE30988
| | Sample_data_row_count | 54675
| | |
|
GSM767919 | GPL570 |
|
HeLa_control_48hours
|
human cervix carcinoma cell line HeLa_control
|
cell line: HeLa
treatment: none
|
|
| Sample_geo_accession | GSM767919
| | Sample_status | Public on May 23 2012
| | Sample_submission_date | Jul 27 2011
| | Sample_last_update_date | May 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For expression analysis, cells were seeded 24 h before drug treatment in 6-Well plates (300,000 cells per well) and were cultured for the respective time points with doxorubicin, DMSO as solvent respectively.
| | Sample_growth_protocol_ch1 | The human cervix carcinoma cell line HeLa were obtained from the American Type Culture Collection and maintained by culture in RPMI 1640 or DMEM medium supplemented with 10% FCS, 1% glutamine, 50 U/ml penicillin, and 50 µg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA of one batch Doxorubicin treated and untreated (DMSO) HeLa cells for two timepoints (24h, 48h) was extracted using Trizol PeqGold RNA Pure (Peqlab, Erlangen, Germany) according to the manufacturer`s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | T7-RNA polymerase-mediated linear amplification was performed starting with 1µg RNA (One cycle Target labelling protocol; Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA, USA) were employed.
| | Sample_scan_protocol | After hybridization and washing, arrays were scanned using the GeneChip System Confocal Scanner 3000 (Affymetrix)
| | Sample_data_processing | Affymetrix default parameters were used for data extraction (GCOS, MAS5.0). Expression data were transferred to GeneSpring GX version 7.3.1. (Agilent Technologies) and per-chip normalized. Genes which were flagged as present or marginal and exceeding a fold change of 2 in comparison to control were further analysed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sabine,,Ameling
| | Sample_contact_laboratory | Functional Genomics
| | Sample_contact_department | Interfaculty Institute of Genetics and Functional Genomics
| | Sample_contact_institute | Ernst-Moritz-Arndt University of Greifswald
| | Sample_contact_address | Jahnstraße 15a
| | Sample_contact_city | Greifswald
| | Sample_contact_zip/postal_code | 17487
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM767nnn/GSM767919/suppl/GSM767919.CEL.gz
| | Sample_series_id | GSE30988
| | Sample_data_row_count | 54675
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