Search results for the GEO ID: GSE31040  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM769038 | GPL570 |
|
LcL, basal, centenarian 1
|
LcL, basal, centenarian
|
cell type: cultured lymphoblastoid cells
gender: female
age: 104 years
culture condition: basal
subject identifier: C139
|
C139_cent_B
|
| Sample_geo_accession | GSM769038
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769038/suppl/GSM769038.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769039 | GPL570 |
|
LcL, starvation, centenarian 1
|
LcL, starvation, centenarian
|
cell type: cultured lymphoblastoid cells
gender: female
age: 104 years
culture condition: starvation
subject identifier: C139
|
C139_cent_R
|
| Sample_geo_accession | GSM769039
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769039/suppl/GSM769039.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769040 | GPL570 |
|
LcL, basal, centenarian 2
|
LcL, basal, centenarian
|
cell type: cultured lymphoblastoid cells
gender: male
age: 100 years
culture condition: basal
subject identifier: C402
|
C402_cent_B
|
| Sample_geo_accession | GSM769040
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769040/suppl/GSM769040.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769041 | GPL570 |
|
LcL, starvation, centenarian 2
|
LcL, starvation, centenarian
|
cell type: cultured lymphoblastoid cells
gender: male
age: 100 years
culture condition: starvation
subject identifier: C402
|
C402_cent_R
|
| Sample_geo_accession | GSM769041
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769041/suppl/GSM769041.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769042 | GPL570 |
|
LcL, basal, young 1
|
LcL, basal, young
|
cell type: cultured lymphoblastoid cells
gender: male
age: 40 years
culture condition: basal
subject identifier: C499
|
C499_ctrl_B
|
| Sample_geo_accession | GSM769042
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769042/suppl/GSM769042.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769043 | GPL570 |
|
LcL, starvation, young 1
|
LcL, starvation, young
|
cell type: cultured lymphoblastoid cells
gender: male
age: 40 years
culture condition: starvation
subject identifier: C499
|
C499_ctrl_R
|
| Sample_geo_accession | GSM769043
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769043/suppl/GSM769043.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769044 | GPL570 |
|
LcL, basal, young 2
|
LcL, basal, young
|
cell type: cultured lymphoblastoid cells
gender: male
age: 28 years
culture condition: basal
subject identifier: C639
|
C639_ctrl_B
|
| Sample_geo_accession | GSM769044
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769044/suppl/GSM769044.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769045 | GPL570 |
|
LcL, starvation, young 2
|
LcL, starvation, young
|
cell type: cultured lymphoblastoid cells
gender: male
age: 28 years
culture condition: starvation
subject identifier: C639
|
C639_ctrl_R
|
| Sample_geo_accession | GSM769045
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769045/suppl/GSM769045.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769046 | GPL570 |
|
LcL, basal, centenarian 3
|
LcL, basal, centenarian
|
cell type: cultured lymphoblastoid cells
gender: male
age: 106 years
culture condition: basal
subject identifier: C645
|
C645_cent_B
|
| Sample_geo_accession | GSM769046
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769046/suppl/GSM769046.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769047 | GPL570 |
|
LcL, starvation, centenarian 3
|
LcL, starvation, centenarian
|
cell type: cultured lymphoblastoid cells
gender: male
age: 106 years
culture condition: starvation
subject identifier: C645
|
C645_cent_R
|
| Sample_geo_accession | GSM769047
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769047/suppl/GSM769047.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769048 | GPL570 |
|
LcL, basal, young 3
|
LcL, basal, young
|
cell type: cultured lymphoblastoid cells
gender: female
age: 37 years
culture condition: basal
subject identifier: C931
|
C931_ctrl_B
|
| Sample_geo_accession | GSM769048
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769048/suppl/GSM769048.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
|
GSM769049 | GPL570 |
|
LcL, starvation, young 3
|
LcL, starvation, young
|
cell type: cultured lymphoblastoid cells
gender: female
age: 37 years
culture condition: starvation
subject identifier: C931
|
C931_ctrl_R
|
| Sample_geo_accession | GSM769049
| | Sample_status | Public on Jul 30 2011
| | Sample_submission_date | Jul 29 2011
| | Sample_last_update_date | Aug 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Starvation was performed by cultivating the cells in the absence of FBS for 48h.
| | Sample_growth_protocol_ch1 | The Lcs were cultured in RPMI medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin (100 mg/ml) in the presence or absence of 10% fetal bovine serum (FBS) for 48 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected and total cellular RNA was isolated by means of the Qiagen RNeasy Micro Kit following the manifacturer's instructions (Qiagen, Valencia, CA). Quantity and purity/integrity of each sample was quantified using the Nanodrop 1000 (Thermo Fisher Scientific, Freemont, CA, USA) and the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled target synthesis was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | HG-U133A GeneChip Plus 2.0 arrays hybridization and staining were performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Scanning was performed according to the GeneChip One-Cycle Target Labeling and Control Reagents protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA, USA).
| | Sample_data_processing | RMA using Partek Genomics Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,,Remondini
| | Sample_contact_email | daniel.remondini@unibo.it
| | Sample_contact_institute | Dip. di Fisica, Bologna University
| | Sample_contact_address | Viale Berti Pichat 6/2
| | Sample_contact_city | Bologna
| | Sample_contact_zip/postal_code | 40127
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769049/suppl/GSM769049.CEL.gz
| | Sample_series_id | GSE31040
| | Sample_data_row_count | 54675
| | |
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