Result

Search results for the GEO ID: GSE31080

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM769723

GPL1355

IL-1b_veh_treated_SMCs

Rat aortic smooth muscle cells treated with IL-1b vehicle (.1% BSA and 10 mM acetic acid)

strain: Sprague-Dawley

Gene expression data from IL-1 vehicle-treated rat aortic smooth muscle cells

Sample_geo_accession	GSM769723
Sample_status	Public on May 03 2012
Sample_submission_date	Aug 01 2011
Sample_last_update_date	May 03 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with IL-1b (2.5 ng/mL; R&D Systems) or vehicle (0.1% bovine serum albumin [BSA] and 10 mM acetic acid), or PDGF-DD (30 ng/mL; Zymogenetics) or vehicle (0.01% BSA) for 24 hours.
Sample_growth_protocol_ch1	Rat aortic SMCs were grown to confluency in serum-containing DMEM/F12 media for 3 days, then serum starved in serum-free media for 3 days, and then were treated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	10 ug of cRNA was hybridized for 16 hours on Affymetrix rat 230 2.0 GeneChips and stained using Affymetrix Fluidics Station 450 according to Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000.
Sample_data_processing	Array normalization and processing was performed using the ExpressionFileCreator module of GenePattern (Broad Institute). MAS5 was used for processing, and normalization was performed with median scaling. Significant differences were defined as fold changes greater or less than or equal to 2 and signal differences in signal intensity greater than or equal to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Gary,,Owens
Sample_contact_laboratory	Gary Owens' Laboratory
Sample_contact_department	Molecular Physiology and Biological Physics
Sample_contact_institute	University of Virginia
Sample_contact_address	415 Lane Road
Sample_contact_city	Charlottesville
Sample_contact_state	VA
Sample_contact_zip/postal_code	22908
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769723/suppl/GSM769723_07-21_veh_A.CEL.gz
Sample_series_id	GSE31080
Sample_data_row_count	31099

GSM769724

GPL1355

IL-1b_treated_SMCs

Rat aortic smooth muscle cells treated with IL-1b (2.5 ng/mL)

strain: Sprague-Dawley

Gene expression data from IL-1b-treated rat aortic smooth muscle cells

Sample_geo_accession	GSM769724
Sample_status	Public on May 03 2012
Sample_submission_date	Aug 01 2011
Sample_last_update_date	May 03 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with IL-1b (2.5 ng/mL; R&D Systems) or vehicle (0.1% bovine serum albumin [BSA] and 10 mM acetic acid), or PDGF-DD (30 ng/mL; Zymogenetics) or vehicle (0.01% BSA) for 24 hours.
Sample_growth_protocol_ch1	Rat aortic SMCs were grown to confluency in serum-containing DMEM/F12 media for 3 days, then serum starved in serum-free media for 3 days, and then were treated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	10 ug of cRNA was hybridized for 16 hours on Affymetrix rat 230 2.0 GeneChips and stained using Affymetrix Fluidics Station 450 according to Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000.
Sample_data_processing	Array normalization and processing was performed using the ExpressionFileCreator module of GenePattern (Broad Institute). MAS5 was used for processing, and normalization was performed with median scaling. Significant differences were defined as fold changes greater or less than or equal to 2 and signal differences in signal intensity greater than or equal to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Gary,,Owens
Sample_contact_laboratory	Gary Owens' Laboratory
Sample_contact_department	Molecular Physiology and Biological Physics
Sample_contact_institute	University of Virginia
Sample_contact_address	415 Lane Road
Sample_contact_city	Charlottesville
Sample_contact_state	VA
Sample_contact_zip/postal_code	22908
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769724/suppl/GSM769724_07-21_IL-1b_A.CEL.gz
Sample_series_id	GSE31080
Sample_data_row_count	31099

GSM769725

GPL1355

PDGF-DD_vehicle_treated_SMCs

Rat aortic smooth muscle cells treated with PDGF-DD vehicle (0.01% BSA)

strain: Sprague-Dawley

Gene expression data from PDGF-DD vehicle-treated rat aortic smooth muscle cells

Sample_geo_accession	GSM769725
Sample_status	Public on May 03 2012
Sample_submission_date	Aug 01 2011
Sample_last_update_date	May 03 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with IL-1b (2.5 ng/mL; R&D Systems) or vehicle (0.1% bovine serum albumin [BSA] and 10 mM acetic acid), or PDGF-DD (30 ng/mL; Zymogenetics) or vehicle (0.01% BSA) for 24 hours.
Sample_growth_protocol_ch1	Rat aortic SMCs were grown to confluency in serum-containing DMEM/F12 media for 3 days, then serum starved in serum-free media for 3 days, and then were treated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	10 ug of cRNA was hybridized for 16 hours on Affymetrix rat 230 2.0 GeneChips and stained using Affymetrix Fluidics Station 450 according to Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000.
Sample_data_processing	Array normalization and processing was performed using the ExpressionFileCreator module of GenePattern (Broad Institute). MAS5 was used for processing, and normalization was performed with median scaling. Significant differences were defined as fold changes greater or less than or equal to 2 and signal differences in signal intensity greater than or equal to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Gary,,Owens
Sample_contact_laboratory	Gary Owens' Laboratory
Sample_contact_department	Molecular Physiology and Biological Physics
Sample_contact_institute	University of Virginia
Sample_contact_address	415 Lane Road
Sample_contact_city	Charlottesville
Sample_contact_state	VA
Sample_contact_zip/postal_code	22908
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769725/suppl/GSM769725_07_32_Veh_A.CEL.gz
Sample_series_id	GSE31080
Sample_data_row_count	31099

GSM769726

GPL1355

PDGF-DD_treated_SMCs

Rat aortic smooth muscle cells treated with PDGF-DD (30 ng/mL)

strain: Sprague-Dawley

Gene expression data from PDGF-DD-treated rat aortic smooth muscle cells

Sample_geo_accession	GSM769726
Sample_status	Public on May 03 2012
Sample_submission_date	Aug 01 2011
Sample_last_update_date	May 03 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with IL-1b (2.5 ng/mL; R&D Systems) or vehicle (0.1% bovine serum albumin [BSA] and 10 mM acetic acid), or PDGF-DD (30 ng/mL; Zymogenetics) or vehicle (0.01% BSA) for 24 hours.
Sample_growth_protocol_ch1	Rat aortic SMCs were grown to confluency in serum-containing DMEM/F12 media for 3 days, then serum starved in serum-free media for 3 days, and then were treated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	10 ug of cRNA was hybridized for 16 hours on Affymetrix rat 230 2.0 GeneChips and stained using Affymetrix Fluidics Station 450 according to Affymetrix protocols.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000.
Sample_data_processing	Array normalization and processing was performed using the ExpressionFileCreator module of GenePattern (Broad Institute). MAS5 was used for processing, and normalization was performed with median scaling. Significant differences were defined as fold changes greater or less than or equal to 2 and signal differences in signal intensity greater than or equal to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Gary,,Owens
Sample_contact_laboratory	Gary Owens' Laboratory
Sample_contact_department	Molecular Physiology and Biological Physics
Sample_contact_institute	University of Virginia
Sample_contact_address	415 Lane Road
Sample_contact_city	Charlottesville
Sample_contact_state	VA
Sample_contact_zip/postal_code	22908
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM769nnn/GSM769726/suppl/GSM769726_07-32_DD_A.CEL.gz
Sample_series_id	GSE31080
Sample_data_row_count	31099

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