Search results for the GEO ID: GSE31117  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM770531 | GPL1355 |
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Cardiomyocytes-G, biological rep1
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Neonatal rat cardiomyocytes infected with Ad-GFP
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strain: Sprague-Dawley
age: 1- to 2-days old
developmental stage: neonate
tissue: heart ventricular myocardium
cell type: cardiomyocyte
infection: control
|
Gene expression data from neonatal rat cardiomyocytes infected with Ad-GFP
Sample name: 3-G
|
| Sample_geo_accession | GSM770531
| | Sample_status | Public on Aug 03 2011
| | Sample_submission_date | Aug 02 2011
| | Sample_last_update_date | Aug 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Cardiomyocytes were infected with GFP control or atrogin-1 adenovirus and cultured with serum-free DMEM/F12 for 24 hours.
| | Sample_growth_protocol_ch1 | Primary cardiomyocytes were prepared by enzymatic disassociation of 1- to 2-day old Sprague-Dawley rats, the isolated cardiomyocytes resuspended in fresh DMEM/F12 (HyClone) containing 10% FBS and 1% penicillin-streptomycin were plated into the Laminin (Sigma) precoated dishes (Corning) and incubated for 24 h at 37 ℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using TRIzol according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat 230 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
| | Sample_data_processing | The data were analyzed with GeneSpring GX 11.0 Software using RMA algorithm.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | junjie,,li
| | Sample_contact_institute | PUMC
| | Sample_contact_address | #5 Dong Dan San Tiao
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100005
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770531/suppl/GSM770531_3-G.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770531/suppl/GSM770531_3-G.rma.chp.gz
| | Sample_series_id | GSE31117
| | Sample_data_row_count | 31099
| | |
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GSM770532 | GPL1355 |
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Cardiomyocytes-G, biological rep2
|
Neonatal rat cardiomyocytes infected with Ad-GFP
|
strain: Sprague-Dawley
age: 1- to 2-days old
developmental stage: neonate
tissue: heart ventricular myocardium
cell type: cardiomyocyte
infection: control
|
Gene expression data from neonatal rat cardiomyocytes infected with Ad-GFP
Sample name: 4-G
|
| Sample_geo_accession | GSM770532
| | Sample_status | Public on Aug 03 2011
| | Sample_submission_date | Aug 02 2011
| | Sample_last_update_date | Aug 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Cardiomyocytes were infected with GFP control or atrogin-1 adenovirus and cultured with serum-free DMEM/F12 for 24 hours.
| | Sample_growth_protocol_ch1 | Primary cardiomyocytes were prepared by enzymatic disassociation of 1- to 2-day old Sprague-Dawley rats, the isolated cardiomyocytes resuspended in fresh DMEM/F12 (HyClone) containing 10% FBS and 1% penicillin-streptomycin were plated into the Laminin (Sigma) precoated dishes (Corning) and incubated for 24 h at 37 ℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using TRIzol according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat 230 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
| | Sample_data_processing | The data were analyzed with GeneSpring GX 11.0 Software using RMA algorithm.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | junjie,,li
| | Sample_contact_institute | PUMC
| | Sample_contact_address | #5 Dong Dan San Tiao
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100005
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770532/suppl/GSM770532_4-G.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770532/suppl/GSM770532_4-G.rma.chp.gz
| | Sample_series_id | GSE31117
| | Sample_data_row_count | 31099
| | |
|
GSM770533 | GPL1355 |
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Cardiomyocytes-A, biological rep1
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Neonatal rat cardiomyocytes infected with Ad-atrogin-1-GFP
|
strain: Sprague-Dawley
age: 1- to 2-days old
developmental stage: neonate
tissue: heart ventricular myocardium
cell type: cardiomyocyte
infection: atrogin-1 adenovirus
|
Gene expression data from neonatal rat cardiomyocytes infected with Ad-atrogin-1-GFP
Sample name: 3-A
|
| Sample_geo_accession | GSM770533
| | Sample_status | Public on Aug 03 2011
| | Sample_submission_date | Aug 02 2011
| | Sample_last_update_date | Aug 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Cardiomyocytes were infected with GFP control or atrogin-1 adenovirus and cultured with serum-free DMEM/F12 for 24 hours.
| | Sample_growth_protocol_ch1 | Primary cardiomyocytes were prepared by enzymatic disassociation of 1- to 2-day old Sprague-Dawley rats, the isolated cardiomyocytes resuspended in fresh DMEM/F12 (HyClone) containing 10% FBS and 1% penicillin-streptomycin were plated into the Laminin (Sigma) precoated dishes (Corning) and incubated for 24 h at 37 ℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using TRIzol according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat 230 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
| | Sample_data_processing | The data were analyzed with GeneSpring GX 11.0 Software using RMA algorithm.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | junjie,,li
| | Sample_contact_institute | PUMC
| | Sample_contact_address | #5 Dong Dan San Tiao
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100005
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770533/suppl/GSM770533_3-A.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770533/suppl/GSM770533_3-A.rma.chp.gz
| | Sample_series_id | GSE31117
| | Sample_data_row_count | 31099
| | |
|
GSM770534 | GPL1355 |
|
Cardiomyocytes-A , biological rep2
|
Neonatal rat cardiomyocytes infected with Ad-atrogin-1-GFP
|
strain: Sprague-Dawley
age: 1- to 2-days old
developmental stage: neonate
tissue: heart ventricular myocardium
cell type: cardiomyocyte
infection: atrogin-1 adenovirus
|
Gene expression data from neonatal rat cardiomyocytes infected with Ad-atrogin-1-GFP
Sample name: 4-A
|
| Sample_geo_accession | GSM770534
| | Sample_status | Public on Aug 03 2011
| | Sample_submission_date | Aug 02 2011
| | Sample_last_update_date | Aug 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Cardiomyocytes were infected with GFP control or atrogin-1 adenovirus and cultured with serum-free DMEM/F12 for 24 hours.
| | Sample_growth_protocol_ch1 | Primary cardiomyocytes were prepared by enzymatic disassociation of 1- to 2-day old Sprague-Dawley rats, the isolated cardiomyocytes resuspended in fresh DMEM/F12 (HyClone) containing 10% FBS and 1% penicillin-streptomycin were plated into the Laminin (Sigma) precoated dishes (Corning) and incubated for 24 h at 37 ℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using TRIzol according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat 230 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
| | Sample_data_processing | The data were analyzed with GeneSpring GX 11.0 Software using RMA algorithm.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | junjie,,li
| | Sample_contact_institute | PUMC
| | Sample_contact_address | #5 Dong Dan San Tiao
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100005
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770534/suppl/GSM770534_4-A.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM770nnn/GSM770534/suppl/GSM770534_4-A.rma.chp.gz
| | Sample_series_id | GSE31117
| | Sample_data_row_count | 31099
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