Search results for the GEO ID: GSE31193 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM773317 | GPL570 |
|
Con_1
|
Con_1
|
tissue: liver
agent: none
time: n/a
|
|
Sample_geo_accession | GSM773317
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773317/suppl/GSM773317.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773317/suppl/GSM773317.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773318 | GPL570 |
|
Con_2
|
Con_2
|
tissue: liver
agent: none
time: n/a
|
|
Sample_geo_accession | GSM773318
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773318/suppl/GSM773318.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773318/suppl/GSM773318.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773319 | GPL570 |
|
Con_3
|
Con_3
|
tissue: liver
agent: none
time: n/a
|
|
Sample_geo_accession | GSM773319
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773319/suppl/GSM773319.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773319/suppl/GSM773319.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773320 | GPL570 |
|
IFN-24_1
|
IFN-24_1
|
tissue: liver
agent: IFN
time: 24
|
|
Sample_geo_accession | GSM773320
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773320/suppl/GSM773320.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773320/suppl/GSM773320.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773321 | GPL570 |
|
IFN-24_2
|
IFN-24_2
|
tissue: liver
agent: IFN
time: 24
|
|
Sample_geo_accession | GSM773321
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773321/suppl/GSM773321.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773321/suppl/GSM773321.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773322 | GPL570 |
|
IFN-24_3
|
IFN-24_3
|
tissue: liver
agent: IFN
time: 24
|
|
Sample_geo_accession | GSM773322
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773322/suppl/GSM773322.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773322/suppl/GSM773322.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773323 | GPL570 |
|
IFN-6_1
|
IFN-6_1
|
tissue: liver
agent: IFN
time: 6
|
|
Sample_geo_accession | GSM773323
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773323/suppl/GSM773323.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773323/suppl/GSM773323.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773324 | GPL570 |
|
IFN-6_2
|
IFN-6_2
|
tissue: liver
agent: IFN
time: 6
|
|
Sample_geo_accession | GSM773324
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773324/suppl/GSM773324.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773324/suppl/GSM773324.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773325 | GPL570 |
|
IFN-6_3
|
IFN-6_3
|
tissue: liver
agent: IFN
time: 6
|
|
Sample_geo_accession | GSM773325
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773325/suppl/GSM773325.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773325/suppl/GSM773325.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773326 | GPL570 |
|
IL28B-24_1
|
IL28B-24_1
|
tissue: liver
agent: IL28B
time: 24
|
|
Sample_geo_accession | GSM773326
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773326/suppl/GSM773326.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773326/suppl/GSM773326.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773327 | GPL570 |
|
IL28B-24_2
|
IL28B-24_2
|
tissue: liver
agent: IL28B
time: 24
|
|
Sample_geo_accession | GSM773327
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773327/suppl/GSM773327.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773327/suppl/GSM773327.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773328 | GPL570 |
|
IL28B-24_3
|
IL28B-24_3
|
tissue: liver
agent: IL28B
time: 24
|
|
Sample_geo_accession | GSM773328
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773328/suppl/GSM773328.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773328/suppl/GSM773328.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773329 | GPL570 |
|
IL28B-6_1
|
IL28B-6_1
|
tissue: liver
agent: IL28B
time: 6
|
|
Sample_geo_accession | GSM773329
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773329/suppl/GSM773329.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773329/suppl/GSM773329.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773330 | GPL570 |
|
IL28B-6_2
|
IL28B-6_2
|
tissue: liver
agent: IL28B
time: 6
|
|
Sample_geo_accession | GSM773330
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773330/suppl/GSM773330.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773330/suppl/GSM773330.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
GSM773331 | GPL570 |
|
IL28B-6_3
|
IL28B-6_3
|
tissue: liver
agent: IL28B
time: 6
|
|
Sample_geo_accession | GSM773331
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 03 2011
| Sample_last_update_date | Mar 09 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization by software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773331/suppl/GSM773331.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM773nnn/GSM773331/suppl/GSM773331.CHP.gz
| Sample_series_id | GSE31193
| Sample_data_row_count | 54675
| |
|
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