Search results for the GEO ID: GSE31264 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM774851 | GPL570 |
|
Control
|
Control
|
treatment: Control
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM774851
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 08 2011
| Sample_last_update_date | May 07 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | MAS5 normalization method of Affymetrix
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774851/suppl/GSM774851_10-30-10_J.Liang1_ET_HG_U133_Plus_1_Control.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774851/suppl/GSM774851_10-30-10_J.Liang1_ET_HG_U133_Plus_1_Control.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM774852 | GPL570 |
|
IFNalpha 6 h
|
IFNalpha
|
treatment: IFNalpha
time: 6 h
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM774852
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 08 2011
| Sample_last_update_date | May 07 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | MAS5 normalization method of Affymetrix
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774852/suppl/GSM774852_10-30-10_J.Liang2_ET_HG_U133_Plus_2_IFNalpha_6Hr.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774852/suppl/GSM774852_10-30-10_J.Liang2_ET_HG_U133_Plus_2_IFNalpha_6Hr.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM774853 | GPL570 |
|
IFNalpha 24 h
|
IFNalpha
|
treatment: IFNalpha
time: 24 h
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM774853
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 08 2011
| Sample_last_update_date | May 07 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | MAS5 normalization method of Affymetrix
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774853/suppl/GSM774853_10-30-10_J.Liang3_ET_HG_U133_Plus_3_IFNalpha_24Hr.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774853/suppl/GSM774853_10-30-10_J.Liang3_ET_HG_U133_Plus_3_IFNalpha_24Hr.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM774854 | GPL570 |
|
IL28B 6 h
|
IL28B
|
treatment: IL28B
time: 6 h
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM774854
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 08 2011
| Sample_last_update_date | May 07 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | MAS5 normalization method of Affymetrix
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774854/suppl/GSM774854_10-30-10_J.Liang4_ET_HG_U133_Plus_4_IL28B_6Hr.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774854/suppl/GSM774854_10-30-10_J.Liang4_ET_HG_U133_Plus_4_IL28B_6Hr.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM774855 | GPL570 |
|
IL28B 24 h
|
IL28B
|
treatment: IL28B
time: 24 h
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM774855
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 08 2011
| Sample_last_update_date | May 07 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | MAS5 normalization method of Affymetrix
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774855/suppl/GSM774855_10-30-10_J.Liang5_ET_HG_U133_Plus_5_IL28B_24Hr.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774855/suppl/GSM774855_10-30-10_J.Liang5_ET_HG_U133_Plus_5_IL28B_24Hr.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM774856 | GPL570 |
|
T Poly IC
|
T Poly IC
|
treatment: T Poly IC
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM774856
| Sample_status | Public on Mar 09 2012
| Sample_submission_date | Aug 08 2011
| Sample_last_update_date | May 07 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | MAS5 normalization method of Affymetrix
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774856/suppl/GSM774856_10-30-10_J.Liang6_ET_HG_U133_Plus_6_T_Poly_IC.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM774nnn/GSM774856/suppl/GSM774856_10-30-10_J.Liang6_ET_HG_U133_Plus_6_T_Poly_IC.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924592 | GPL570 |
|
Con_a_4_JFHa-1
|
Con_a_4_JFHa
|
treatment: none
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924592
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924592/suppl/GSM924592_08-12-10_JLiang1_ET_HG_U133Plus_2_1-1Cona.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924592/suppl/GSM924592_08-12-10_JLiang1_ET_HG_U133Plus_2_1-1Cona.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924593 | GPL570 |
|
Con_a_4_JFHa-2
|
Con_a_4_JFHa
|
treatment: none
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924593
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924593/suppl/GSM924593_08-12-10_JLiang2_ET_HG_U133Plus_2_2a-1Cona.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924593/suppl/GSM924593_08-12-10_JLiang2_ET_HG_U133Plus_2_2a-1Cona.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924594 | GPL570 |
|
Con_a_4_JFHa-3
|
Con_a_4_JFHa
|
treatment: none
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924594
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924594/suppl/GSM924594_08-12-10_JLiang3_ET_HG_U133Plus_2_3-1Cona.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924594/suppl/GSM924594_08-12-10_JLiang3_ET_HG_U133Plus_2_3-1Cona.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924595 | GPL570 |
|
JFH_a-1
|
JFH_a
|
treatment: MOI of 5
time: 36 hours
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924595
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924595/suppl/GSM924595_08-12-10_JLiang4_ET_HG_U133Plus_2_4-19JFHa.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924595/suppl/GSM924595_08-12-10_JLiang4_ET_HG_U133Plus_2_4-19JFHa.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924596 | GPL570 |
|
JFH_a-2
|
JFH_a
|
treatment: MOI of 5
time: 36 hours
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924596
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924596/suppl/GSM924596_08-12-10_JLiang5_ET_HG_U133Plus_2_5-20JFHa.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924596/suppl/GSM924596_08-12-10_JLiang5_ET_HG_U133Plus_2_5-20JFHa.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924597 | GPL570 |
|
JFH_a-3
|
JFH_a
|
treatment: MOI of 5
time: 36 hours
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924597
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924597/suppl/GSM924597_08-12-10_JLiang6_ET_HG_U133Plus_2_6-20JFHa.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924597/suppl/GSM924597_08-12-10_JLiang6_ET_HG_U133Plus_2_6-20JFHa.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924598 | GPL570 |
|
Con_a_4_IL28B-1_09-30-10
|
Con_a_4_IL28B-_09-30-10
|
treatment: none
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924598
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924598/suppl/GSM924598_09-30-10_J.Liang1_ET_HG_U133_Plus_1_1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924598/suppl/GSM924598_09-30-10_J.Liang1_ET_HG_U133_Plus_1_1.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924599 | GPL570 |
|
Con_a_4_IL28B-2_09-30-10
|
Con_a_4_IL28B-_09-30-10
|
treatment: none
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924599
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924599/suppl/GSM924599_09-30-10_J.Liang2_ET_HG_U133_Plus_2_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924599/suppl/GSM924599_09-30-10_J.Liang2_ET_HG_U133_Plus_2_2.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924600 | GPL570 |
|
Con_a_4_IL28B-3_09-30-10
|
Con_a_4_IL28B-_09-30-10
|
treatment: none
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924600
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924600/suppl/GSM924600_09-30-10_J.Liang3_ET_HG_U133_Plus_3_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924600/suppl/GSM924600_09-30-10_J.Liang3_ET_HG_U133_Plus_3_2.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924601 | GPL570 |
|
09-30-10_IL28b-1
|
09-30-10_IL28b
|
treatment: recombinant IL28B (25 ng/mL)
time: 24 hours
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924601
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924601/suppl/GSM924601_09-30-10_J.Liang4_ET_HG_U133_Plus_4_15.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924601/suppl/GSM924601_09-30-10_J.Liang4_ET_HG_U133_Plus_4_15.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924602 | GPL570 |
|
09-30-10_IL28b-2
|
09-30-10_IL28b
|
treatment: recombinant IL28B (25 ng/mL)
time: 24 hours
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924602
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924602/suppl/GSM924602_09-30-10_J.Liang5_ET_HG_U133_Plus_5_16.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924602/suppl/GSM924602_09-30-10_J.Liang5_ET_HG_U133_Plus_5_16.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
GSM924603 | GPL570 |
|
09-30-10_IL28b-3
|
09-30-10_IL28b
|
treatment: recombinant IL28B (25 ng/mL)
time: 24 hours
cell type: primary hepatocyte
|
Samples collected from Human liver
|
Sample_geo_accession | GSM924603
| Sample_status | Public on May 08 2012
| Sample_submission_date | Apr 27 2012
| Sample_last_update_date | May 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after recombinant IL28B (25 ng/mL for 24 hours) and JFH1 (MOI of 5 for 36 hours) with three replications.
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA normalization method of software Parteck
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924603/suppl/GSM924603_09-30-10_J.Liang6_ET_HG_U133_Plus_6_16.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM924nnn/GSM924603/suppl/GSM924603_09-30-10_J.Liang6_ET_HG_U133_Plus_6_16.CHP.gz
| Sample_series_id | GSE31264
| Sample_data_row_count | 54675
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
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