Result

Search results for the GEO ID: GSE31359

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM777543

GPL1261

NC-EpH4

Mammary gland epithelial cells (EMT resistance)

tissue: Mammary gland treatment: resting epithelial cells

Gene expression data from resting epithelial cells

Sample_geo_accession	GSM777543
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777543/suppl/GSM777543_2654-G1-NC-EpH4.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777544

GPL1261

EMT-EpH4

EMT-induced mammary gland epithelial cells (EMT resistance)

tissue: Mammary gland treatment: epithelial cells stimulated with TGF-b1/EGF

Gene expression data from epithelial cells stimulated with TGF-b1/EGF

Sample_geo_accession	GSM777544
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777544/suppl/GSM777544_2655-G1-EMT-EpH4.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777545

GPL1261

NC-mProx

Proximal renal tubular epithelial cells

tissue: Kidney treatment: resting epithelial cells

Gene expression data from resting epithelial cells

Sample_geo_accession	GSM777545
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777545/suppl/GSM777545_2656-G1-2328NC_mProx.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777546

GPL1261

EMT-mProx

EMT-induced proximal renal tubular epithelial cells

tissue: Kidney treatment: epithelial cells stimulated with TGF-b1/EGF

Gene expression data from epithelial cells stimulated with TGF-b1/EGF

Sample_geo_accession	GSM777546
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777546/suppl/GSM777546_2657-G1-2329EMT_mProx.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777547

GPL1261

NC-MCT

Renal tubular epithelial cells

tissue: Kidney treatment: resting epithelial cells

Gene expression data from resting epithelial cells

Sample_geo_accession	GSM777547
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777547/suppl/GSM777547_2658-G1-2398NC-MCT.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777548

GPL1261

EMT-MCT

EMT-induced renal tubular epithelial cells

tissue: Kidney treatment: epithelial cells stimulated with TGF-b1/EGF

Gene expression data from epithelial cells stimulated with TGF-b1/EGF

Sample_geo_accession	GSM777548
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777548/suppl/GSM777548_2659-G1-2399EMT-MCT.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777549

GPL1261

NC-NMuMG

Mammary gland epithelial cells

tissue: Mammary gland treatment: resting epithelial cells

Gene expression data from resting epithelial cells

Sample_geo_accession	GSM777549
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777549/suppl/GSM777549_2660-G1-2400NC-NMuMG.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

GSM777550

GPL1261

EMT-NMuMG

EMT-induced mammary gland epithelial cells

tissue: Mammary gland treatment: epithelial cells stimulated with TGF-b1/EGF

Gene expression data from epithelial cells stimulated with TGF-b1/EGF

Sample_geo_accession	GSM777550
Sample_status	Public on Aug 13 2011
Sample_submission_date	Aug 12 2011
Sample_last_update_date	Aug 13 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Treated with or without rhTGF-b1 3.0 ng/mL and rhEGF 3.0 ng/mL in K-1 medium for 24 hours.
Sample_growth_protocol_ch1	Seeded in six-well plates (1x105 cells/well) and incubated overnight in DMEM containing 10% FCS, after which the medium was replaced with modified K-1 medium (50:50 Ham's F-12/DMEM with 5μg/ml transferrin and 5μg/ml insulin), and the cells were incubated for further 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by using RNeasy mini-kit (Qiagen) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labelled.
Sample_hyb_protocol	Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akihiro,,Umezawa
Sample_contact_email	umezawa@1985.jukuin.keio.ac.jp
Sample_contact_phone	81-3-5494-7047
Sample_contact_fax	81-3-5494-7048
Sample_contact_department	Reproductive Biology
Sample_contact_institute	National Center for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_contact_web_link	http://1985.jukuin.keio.ac.jp/umezawa/HTML
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM777nnn/GSM777550/suppl/GSM777550_2661-G1-2401EMT-NMuMG.CEL.gz
Sample_series_id	GSE31359
Sample_data_row_count	45101

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