Result

Search results for the GEO ID: GSE31378

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Title

Source name

Description

Characteristics

GSM778255

GPL1261

HRSV infected mouse macrophage at 0H, biological rep1

Mock infected mouse macrophage rep 1

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 0 hpi

Gene expression data from mock infected mouse macrophage rep 1

Sample_geo_accession	GSM778255
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778255/suppl/GSM778255_Mouse_Macrophage_HRSV_0H-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778255/suppl/GSM778255_Mouse_Macrophage_HRSV_0H-1.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778256

GPL1261

HRSV infected mouse macrophage at 0H, biological rep2

Mock infected mouse macrophage rep 2

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 0 hpi

Gene expression data from mock infected mouse macrophage rep 2

Sample_geo_accession	GSM778256
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778256/suppl/GSM778256_Mouse_Macrophage_HRSV_0H-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778256/suppl/GSM778256_Mouse_Macrophage_HRSV_0H-2.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778257

GPL1261

HRSV infected mouse macrophage at 0H, biological rep3

Mock infected mouse macrophage rep 3

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 0 hpi

Gene expression data from mock infected mouse macrophage rep 3

Sample_geo_accession	GSM778257
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778257/suppl/GSM778257_Mouse_Macrophage_HRSV_0H-3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778257/suppl/GSM778257_Mouse_Macrophage_HRSV_0H-3.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778258

GPL1261

HRSV infected mouse macrophage at 4H, biological rep1

HRSV infected mouse macrophage 4 hpi rep 1

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 4 hpi

Gene expression data from HRSV infected mouse macrophage 4 hpi rep 1

Sample_geo_accession	GSM778258
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778258/suppl/GSM778258_Mouse_Macrophage_HRSV_4H-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778258/suppl/GSM778258_Mouse_Macrophage_HRSV_4H-1.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778259

GPL1261

HRSV infected mouse macrophage at 4H, biological rep2

HRSV infected mouse macrophage 4 hpi rep 2

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 4 hpi

Gene expression data from HRSV infected mouse macrophage 4 hpi rep 2

Sample_geo_accession	GSM778259
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778259/suppl/GSM778259_Mouse_Macrophage_HRSV_4H-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778259/suppl/GSM778259_Mouse_Macrophage_HRSV_4H-2.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778260

GPL1261

HRSV infected mouse macrophage at 4H, biological rep3

HRSV infected mouse macrophage 4 hpi rep 3

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 4 hpi

Gene expression data from HRSV infected mouse macrophage 4 hpi rep 3

Sample_geo_accession	GSM778260
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778260/suppl/GSM778260_Mouse_Macrophage_HRSV_4H-3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778260/suppl/GSM778260_Mouse_Macrophage_HRSV_4H-3.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778261

GPL1261

HRSV infected mouse macrophage at 24H, biological rep1

HRSV infected mouse macrophage 24 hpi rep 1

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 24 hpi

Gene expression data from HRSV infected mouse macrophage 24 hpi rep 1

Sample_geo_accession	GSM778261
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778261/suppl/GSM778261_Mouse_Macrophage_HRSV_24H-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778261/suppl/GSM778261_Mouse_Macrophage_HRSV_24H-1.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778262

GPL1261

HRSV infected mouse macrophage at 24H, biological rep2

HRSV infected mouse macrophage 24 hpi rep 2

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 24 hpi

Gene expression data from HRSV infected mouse macrophage 24 hpi rep 2

Sample_geo_accession	GSM778262
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778262/suppl/GSM778262_Mouse_Macrophage_HRSV_24H-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778262/suppl/GSM778262_Mouse_Macrophage_HRSV_24H-2.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

GSM778263

GPL1261

HRSV infected mouse macrophage at 24H, biological rep3

HRSV infected mouse macrophage 24 hpi rep 3

cell type: macrophage CD11b+ cells infection: Human respiratory syncytial virus time: 24 hpi

Gene expression data from HRSV infected mouse macrophage 24 hpi rep 3

Sample_geo_accession	GSM778263
Sample_status	Public on Aug 16 2013
Sample_submission_date	Aug 15 2011
Sample_last_update_date	Aug 16 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	The cells were infected with HRSV MOI=5 in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_growth_protocol_ch1	The cells were grown in DMEM L929 (30%) cell condition media (Gibco) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. The normalization was done using internal and external control genes as assigned by Affymetrix. Further analysis was done in GeneSpring 11.0. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
Sample_platform_id	GPL1261
Sample_contact_name	Richard,J,Sugrue
Sample_contact_email	RJSUGRUE@ntu.edu.sg
Sample_contact_department	Divison of Molecular and Cell Biology
Sample_contact_institute	Nanyang Technological University
Sample_contact_address	60 Nanyang Drive
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	637551
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778263/suppl/GSM778263_Mouse_Macrophage_HRSV_24H-3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778263/suppl/GSM778263_Mouse_Macrophage_HRSV_24H-3.CHP.gz
Sample_series_id	GSE31378
Sample_series_id	GSE31524
Sample_data_row_count	45101

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Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes