Result

Search results for the GEO ID: GSE31399

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Title

Source name

Description

Characteristics

GSM778859

GPL1355

Vehicle, biological rep3

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set II)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: vehicle

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set II)

Sample_geo_accession	GSM778859
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778859/suppl/GSM778859.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778860

GPL1355

Vehicle, biological rep4

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set II)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: vehicle

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set II)

Sample_geo_accession	GSM778860
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778860/suppl/GSM778860.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778861

GPL1355

Vehicle, biological rep5

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set III)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: vehicle

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set III)

Sample_geo_accession	GSM778861
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778861/suppl/GSM778861.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778862

GPL1355

Vehicle, biological rep6

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set III)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: vehicle

Primary rat gonadotrope cells, vehicle, 40 min (Enrichment set III)

Sample_geo_accession	GSM778862
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778862/suppl/GSM778862.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778865

GPL1355

GnRH, biological rep3

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set II)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: 10 nM GnRH

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set II)

Sample_geo_accession	GSM778865
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778865/suppl/GSM778865.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778866

GPL1355

GnRH, biological rep4

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set II)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: 10 nM GnRH

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set II)

Sample_geo_accession	GSM778866
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778866/suppl/GSM778866.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778867

GPL1355

GnRH, biological rep5

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set III)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: 10 nM GnRH

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set III)

Sample_geo_accession	GSM778867
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778867/suppl/GSM778867.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

GSM778868

GPL1355

GnRH, biological rep6

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set III)

cell type: enriched gonadotropes strain: Sprague-Dawley gender: Female age: adult agent: 10 nM GnRH

Primary rat gonadotrope cells, 10 nM GnRH, 40 min (Enrichment set III)

Sample_geo_accession	GSM778868
Sample_status	Public on Aug 17 2011
Sample_submission_date	Aug 16 2011
Sample_last_update_date	Aug 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	On day 3 of culture, GnRH was added to experimental wells to achieve a final concentration of 10 nM; control wells received an equivalent volume of vehicle medium. After 40 min, cells were rinsed with ice-cold PBS, lysis buffer containing guanidinium thiocyanate was added, cells were scraped, triturated, and stored at -70ºC.
Sample_growth_protocol_ch1	Anterior pituitaries (13-15/experiment) from rats ovariectomized for two weeks were enzymatically dispersed with trypsin and resuspended in Ca2+-free Minimum Essential Medium (MEM) containing 0.5% BSA and loaded into a unit gravity sedimentation chamber containing a gradient of 1.0-3.0% BSA in Ca2+-free MEM. After equilibration, cells were collected from the bottom of the gradient in 20x50 ml fractions. Fractions 17-20 contained large cells and were combined; this pool represented ~6% of the total cells recovered from the sedimentation chamber. As determined by subsequent immunocytochemistry, LH-positive cells in this pool ranged from 40-60% compared to < 7% for unfractionated cells. The gonadotrope-enriched pool was pelleted and resuspended in supplemented MEM culture medium [MEM plus 200 µM kanamycin sulfate, 0.2 nM E2, and 10% fetal bovine serum (FBS) that had been charcoal-treated to remove endogenous steroids].
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cell lysates were processed for total RNA using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA) and included an on-column DNase I digestion step.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix two round T7 amplification.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 v2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1355
Sample_contact_name	Hanna,,Pincas
Sample_contact_email	hanna.pincas@mssm.edu
Sample_contact_phone	212-241-1750
Sample_contact_fax	212-289-4107
Sample_contact_laboratory	Sealfon
Sample_contact_department	Neurology
Sample_contact_institute	Mount Sinai School of Medicine
Sample_contact_address	One Gustave L. Levy Place
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10029
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM778nnn/GSM778868/suppl/GSM778868.CEL.gz
Sample_series_id	GSE31399
Sample_data_row_count	31099

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