Search results for the GEO ID: GSE31434 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM780744 | GPL570 |
|
Negative control, biological rep 1
|
Negative control
|
cell line: HeLa
genotype/variation: transfected with negative control
|
Gene expression data from HeLa cells transfected with negative control.
|
Sample_geo_accession | GSM780744
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780744/suppl/GSM780744_NC4.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780745 | GPL570 |
|
Negative control, biological rep 2
|
Negative control
|
cell line: HeLa
genotype/variation: transfected with negative control
|
Gene expression data from HeLa cells transfected with negative control.
|
Sample_geo_accession | GSM780745
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780745/suppl/GSM780745_NC5.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780746 | GPL570 |
|
Negative control, biological rep 3
|
Negative control
|
cell line: HeLa
genotype/variation: transfected with negative control
|
Gene expression data from HeLa cells transfected with negative control.
|
Sample_geo_accession | GSM780746
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780746/suppl/GSM780746_NC6.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780747 | GPL570 |
|
siSLC44A5, biological rep 1
|
siSLC44A5
|
cell line: HeLa
genotype/variation: transfected with siRNA against SLC44A5
|
Gene expression data from HeLa cells transfected with siRNA against SLC44A5.
|
Sample_geo_accession | GSM780747
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780747/suppl/GSM780747_siSLC1.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780748 | GPL570 |
|
siSLC44A5, biological rep 2
|
siSLC44A5
|
cell line: HeLa
genotype/variation: transfected with siRNA against SLC44A5
|
Gene expression data from HeLa cells transfected with siRNA against SLC44A5.
|
Sample_geo_accession | GSM780748
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780748/suppl/GSM780748_siSLC2.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780749 | GPL570 |
|
siSLC44A5, biological rep 3
|
siSLC44A5
|
cell line: HeLa
genotype/variation: transfected with siRNA against SLC44A5
|
Gene expression data from HeLa cells transfected with siRNA against SLC44A5.
|
Sample_geo_accession | GSM780749
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780749/suppl/GSM780749_siSLC3.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780750 | GPL570 |
|
SLC44A5, biological rep 1
|
SLC44A5
|
cell line: HeLa
genotype/variation: transfected with bovine SLC44A5 expression plasmid
|
Gene expression data from HeLa cells transfected with bovine SLC44A5 expression plasmid.
|
Sample_geo_accession | GSM780750
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780750/suppl/GSM780750_SLC1.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780751 | GPL570 |
|
SLC44A5, biological rep 2
|
SLC44A5
|
cell line: HeLa
genotype/variation: transfected with bovine SLC44A5 expression plasmid
|
Gene expression data from HeLa cells transfected with bovine SLC44A5 expression plasmid.
|
Sample_geo_accession | GSM780751
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780751/suppl/GSM780751_SLC2.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780752 | GPL570 |
|
SLC44A5, biological rep 3
|
SLC44A5
|
cell line: HeLa
genotype/variation: transfected with bovine SLC44A5 expression plasmid
|
Gene expression data from HeLa cells transfected with bovine SLC44A5 expression plasmid.
|
Sample_geo_accession | GSM780752
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780752/suppl/GSM780752_SLC3.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780753 | GPL570 |
|
Vector, biological rep 1
|
Vector
|
cell line: HeLa
genotype/variation: transfected with an empty vector control
|
Gene expression data from HeLa cells transfected with an empty vector control.
|
Sample_geo_accession | GSM780753
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780753/suppl/GSM780753_VEC1.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780754 | GPL570 |
|
Vector, biological rep 2
|
Vector
|
cell line: HeLa
genotype/variation: transfected with an empty vector control
|
Gene expression data from HeLa cells transfected with an empty vector control.
|
Sample_geo_accession | GSM780754
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780754/suppl/GSM780754_VEC2.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
|
GSM780755 | GPL570 |
|
Vector, biological rep 3
|
Vector
|
cell line: HeLa
genotype/variation: transfected with an empty vector control
|
Gene expression data from HeLa cells transfected with an empty vector control.
|
Sample_geo_accession | GSM780755
| Sample_status | Public on Sep 17 2012
| Sample_submission_date | Aug 17 2011
| Sample_last_update_date | Sep 17 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HeLa cells were transfected with Lipofectamine RNAiMAX (Invitrogen) or Lipofectamine 2000 (Invitrogen).
| Sample_growth_protocol_ch1 | HeLa cells were grown at at 37C in an atmosphere of 5 % CO2 and 95 % air.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with R using RMA as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Mayumi,,Sugimoto
| Sample_contact_email | m0komats@nlbc.go.jp
| Sample_contact_institute | National Livestock Breeding Center
| Sample_contact_address | Odakurahara 1 Odakura
| Sample_contact_city | Nishigo
| Sample_contact_state | Fukushima
| Sample_contact_zip/postal_code | 961-8511
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM780nnn/GSM780755/suppl/GSM780755_VEC3.CEL.gz
| Sample_series_id | GSE31434
| Sample_data_row_count | 54675
| |
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