Search results for the GEO ID: GSE31455 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM781696 | GPL570 |
|
Con_JFHA_1
|
Con_JFHA_1
|
treatment: Control
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781696
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781696/suppl/GSM781696.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781696/suppl/GSM781696.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781697 | GPL570 |
|
Con_JFHA_2
|
Con_JFHA_2
|
treatment: Control
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781697
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781697/suppl/GSM781697.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781697/suppl/GSM781697.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781698 | GPL570 |
|
Con_JFHA_3
|
Con_JFHA_3
|
treatment: Control
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781698
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781698/suppl/GSM781698.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781698/suppl/GSM781698.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781699 | GPL570 |
|
JFHA_1
|
JFHA_1
|
treatment: JFHA
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781699
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781699/suppl/GSM781699.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781699/suppl/GSM781699.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781700 | GPL570 |
|
JFHA_2
|
JFHA_2
|
treatment: JFHA
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781700
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781700/suppl/GSM781700.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781700/suppl/GSM781700.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781701 | GPL570 |
|
JFHA_3
|
JFHA_3
|
treatment: JFHA
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781701
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781701/suppl/GSM781701.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781701/suppl/GSM781701.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781702 | GPL570 |
|
CON_4_IL28b_1
|
CON_4_IL28b_1
|
treatment: Control
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781702
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781702/suppl/GSM781702.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781702/suppl/GSM781702.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781703 | GPL570 |
|
CON_4_IL28b_2
|
CON_4_IL28b_2
|
treatment: Control
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781703
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781703/suppl/GSM781703.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781703/suppl/GSM781703.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781704 | GPL570 |
|
CON_4_IL28b_3
|
CON_4_IL28b_3
|
treatment: Control
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781704
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781704/suppl/GSM781704.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781704/suppl/GSM781704.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781705 | GPL570 |
|
IL28b-1
|
IL28b-1
|
treatment: IL28b
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781705
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781705/suppl/GSM781705.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781705/suppl/GSM781705.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
|
GSM781706 | GPL570 |
|
IL28b-2
|
IL28b-2
|
treatment: IL28b
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781706
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781706/suppl/GSM781706.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781706/suppl/GSM781706.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
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GSM781707 | GPL570 |
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IL28b-3
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IL28b-3
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treatment: IL28b
tissue: liver
cell type: hepatocytes
|
|
Sample_geo_accession | GSM781707
| Sample_status | Public on Aug 16 2013
| Sample_submission_date | Aug 18 2011
| Sample_last_update_date | Aug 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RNA was isolated after treatment with IFHA (10 U/mL) or IL28B (25 ng/mL)
| Sample_growth_protocol_ch1 | Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA nomanization method of software Partek
| Sample_platform_id | GPL570
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781707/suppl/GSM781707.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781707/suppl/GSM781707.CHP.gz
| Sample_series_id | GSE31455
| Sample_data_row_count | 54675
| |
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