Search results for the GEO ID: GSE31469  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM781957 | GPL570 |
|
H1N1 infected A549 cells at 0H, biological rep1
|
Mock infected A549 rep 1
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: mock
time: control
|
Gene expression data from mock infected A549 rep 1
|
| Sample_geo_accession | GSM781957
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781957/suppl/GSM781957_A549_H1N1_0H-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781957/suppl/GSM781957_A549_H1N1_0H-1.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781958 | GPL570 |
|
H1N1 infected A549 cells at 0H, biological rep2
|
Mock infected A549 rep 2
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: mock
time: control
|
Gene expression data from mock infected A549 rep 2
|
| Sample_geo_accession | GSM781958
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781958/suppl/GSM781958_A549_H1N1_0H-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781958/suppl/GSM781958_A549_H1N1_0H-2.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781959 | GPL570 |
|
H1N1 infected A549 cells at 0H, biological rep3
|
Mock infected A549 rep 3
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: mock
time: control
|
Gene expression data from mock infected A549 rep 3
|
| Sample_geo_accession | GSM781959
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781959/suppl/GSM781959_A549_H1N1_0H-3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781959/suppl/GSM781959_A549_H1N1_0H-3.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781960 | GPL570 |
|
H1N1 infected A549 cells at 2H, biological rep1
|
H1N1 infected A549 2 hpi rep 1
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 2 hpi
|
Gene expression data from H1N1 infected A549 2 hpi rep 1
|
| Sample_geo_accession | GSM781960
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781960/suppl/GSM781960_A549_H1N1_2H-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781960/suppl/GSM781960_A549_H1N1_2H-1.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781961 | GPL570 |
|
H1N1 infected A549 cells at 2H, biological rep2
|
H1N1 infected A549 2 hpi rep 2
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 2 hpi
|
Gene expression data from H1N1 infected A549 2 hpi rep 2
|
| Sample_geo_accession | GSM781961
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781961/suppl/GSM781961_A549_H1N1_2H-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781961/suppl/GSM781961_A549_H1N1_2H-2.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781962 | GPL570 |
|
H1N1 infected A549 cells at 2H, biological rep3
|
H1N1 infected A549 2 hpi rep 3
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 2 hpi
|
Gene expression data from H1N1 infected A549 2 hpi rep 3
|
| Sample_geo_accession | GSM781962
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781962/suppl/GSM781962_A549_H1N1_2H-3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781962/suppl/GSM781962_A549_H1N1_2H-3.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781963 | GPL570 |
|
H1N1 infected A549 cells at 4H, biological rep1
|
H1N1 infected A549 4 hpi rep 1
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 4 hpi
|
Gene expression data from H1N1 infected A549 4 hpi rep 1
|
| Sample_geo_accession | GSM781963
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781963/suppl/GSM781963_A549_H1N1_4H-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781963/suppl/GSM781963_A549_H1N1_4H-1.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781964 | GPL570 |
|
H1N1 infected A549 cells at 4H, biological rep2
|
H1N1 infected A549 4 hpi rep 2
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 4 hpi
|
Gene expression data from H1N1 infected A549 4 hpi rep 2
|
| Sample_geo_accession | GSM781964
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781964/suppl/GSM781964_A549_H1N1_4H-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781964/suppl/GSM781964_A549_H1N1_4H-2.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781965 | GPL570 |
|
H1N1 infected A549 cells at 4H, biological rep3
|
H1N1 infected A549 4 hpi rep 3
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 6 hpi
|
Gene expression data from H1N1 infected A549 4 hpi rep 3
|
| Sample_geo_accession | GSM781965
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781965/suppl/GSM781965_A549_H1N1_4H-3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781965/suppl/GSM781965_A549_H1N1_4H-3.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781966 | GPL570 |
|
H1N1 infected A549 cells at 6H, biological rep1
|
H1N1 infected A549 6 hpi rep 1
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 6 hpi
|
Gene expression data from H1N1 infected A549 6 hpi rep 1
|
| Sample_geo_accession | GSM781966
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781966/suppl/GSM781966_A549_H1N1_6H-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781966/suppl/GSM781966_A549_H1N1_6H-1.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781967 | GPL570 |
|
H1N1 infected A549 cells at 6H, biological rep2
|
H1N1 infected A549 6 hpi rep 2
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 6 hpi
|
Gene expression data from H1N1 infected A549 6 hpi rep 2
|
| Sample_geo_accession | GSM781967
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781967/suppl/GSM781967_A549_H1N1_6H-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781967/suppl/GSM781967_A549_H1N1_6H-2.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
|
GSM781968 | GPL570 |
|
H1N1 infected A549 cells at 6H, biological rep3
|
H1N1 infected A549 6 hpi rep 3
|
cell type: Human aveolar basal epithelial cells
cell line: A549
infection: H1N1
time: 6 hpi
|
Gene expression data from H1N1 infected A549 6 hpi rep 3
|
| Sample_geo_accession | GSM781968
| | Sample_status | Public on Jan 03 2013
| | Sample_submission_date | Aug 18 2011
| | Sample_last_update_date | Jan 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were infected with A/WSN/33 (H1N1) in DMEM + Glutamax (Gibco) with 2% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_growth_protocol_ch1 | The cells were grown in DMEM + Glutamax (Gibco) with 10% Fetal Bovine Serum (FBS) and 100 units/ml of Penicillin/Sreptomycin (Gibco), at 37˚C, 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using RNAeasy MiniKit (Qiagen) according to the manufacturer's instruction at 4˚C
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated mRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of aRNA were hybridized for 16 hr at 45˚C on Human U133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 5.0 (GCOS 5.0) using Affymetrix default analysis settings. Further analysis was done in GeneSpring 11.0. The normalization was done using internal and external control genes as assigned by Affymetrix. The target intensity of each array on a given time point was first filtered by the flagging of Present (P) in 3 replicates at one time-point, which then further calculated in fold change up- or downregulation with respect to the signal intensity in MOCK infected cells, after analyzed by ANOVA with p value ≤ 0.05.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,J,Sugrue
| | Sample_contact_email | RJSUGRUE@ntu.edu.sg
| | Sample_contact_department | Divison of Molecular and Cell Biology
| | Sample_contact_institute | Nanyang Technological University
| | Sample_contact_address | 60 Nanyang Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 637551
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781968/suppl/GSM781968_A549_H1N1_6H-3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM781nnn/GSM781968/suppl/GSM781968_A549_H1N1_6H-3.CHP.gz
| | Sample_series_id | GSE31469
| | Sample_series_id | GSE31524
| | Sample_data_row_count | 54675
| | |
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