Search results for the GEO ID: GSE31611  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM785147 | GPL1261 |
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WT
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embroid body with wild type BRCA1 gene
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cell type: embryoid body
strain: 129S7/SvEv Brd-Hprt b-m2
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gene expression data from mouse embroid body with wild type human BRCA1
Chang_WT_022908.CEL
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| Sample_geo_accession | GSM785147
| | Sample_status | Public on Dec 31 2011
| | Sample_submission_date | Aug 23 2011
| | Sample_last_update_date | Dec 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Embroid bodies were collected on day 7.
| | Sample_growth_protocol_ch1 | To generate embryoid bodies, ES cells were trypsinized and the feeder cells were removed by incubating the cells on gelatinized plate for one hour. The ES cells in supernatant were counted and diluted to 5X104 cells/ml. The cell suspension was culture in Petri dish, in DMEM-10 media. The media was changed on day 3 and later it was changed every other day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Partek Genomic Suite using default RMA method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Suhwan,,Chang
| | Sample_contact_institute | National Cancer Institute
| | Sample_contact_address | PO Box B, NCI-Frederick
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785147/suppl/GSM785147.CEL.gz
| | Sample_series_id | GSE31611
| | Sample_series_id | GSE31637
| | Sample_data_row_count | 45101
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GSM785148 | GPL1261 |
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mutant MI
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embroid body with MI mutation in BRCA1 gene
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cell type: embryoid body
strain: 129S7/SvEv Brd-Hprt b-m2
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gene expression data from mouse embroid body with M1652I mutation of human BRCA1
Chang_MI_022908.CEL
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| Sample_geo_accession | GSM785148
| | Sample_status | Public on Dec 31 2011
| | Sample_submission_date | Aug 23 2011
| | Sample_last_update_date | Dec 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Embroid bodies were collected on day 7.
| | Sample_growth_protocol_ch1 | To generate embryoid bodies, ES cells were trypsinized and the feeder cells were removed by incubating the cells on gelatinized plate for one hour. The ES cells in supernatant were counted and diluted to 5X104 cells/ml. The cell suspension was culture in Petri dish, in DMEM-10 media. The media was changed on day 3 and later it was changed every other day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Partek Genomic Suite using default RMA method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Suhwan,,Chang
| | Sample_contact_institute | National Cancer Institute
| | Sample_contact_address | PO Box B, NCI-Frederick
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785148/suppl/GSM785148.CEL.gz
| | Sample_series_id | GSE31611
| | Sample_series_id | GSE31637
| | Sample_data_row_count | 45101
| | |
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GSM785149 | GPL1261 |
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mutant RQ
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embroid body with RQ mutation in BRCA1 gene
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cell type: embryoid body
strain: 129S7/SvEv Brd-Hprt b-m2
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gene expression data from mouse embroid body with R1669Q mutation of human BRCA1
Chang_RQ_022908.CEL
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| Sample_geo_accession | GSM785149
| | Sample_status | Public on Dec 31 2011
| | Sample_submission_date | Aug 23 2011
| | Sample_last_update_date | Dec 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Embroid bodies were collected on day 7.
| | Sample_growth_protocol_ch1 | To generate embryoid bodies, ES cells were trypsinized and the feeder cells were removed by incubating the cells on gelatinized plate for one hour. The ES cells in supernatant were counted and diluted to 5X104 cells/ml. The cell suspension was culture in Petri dish, in DMEM-10 media. The media was changed on day 3 and later it was changed every other day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Partek Genomic Suite using default RMA method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Suhwan,,Chang
| | Sample_contact_institute | National Cancer Institute
| | Sample_contact_address | PO Box B, NCI-Frederick
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785149/suppl/GSM785149.CEL.gz
| | Sample_series_id | GSE31611
| | Sample_series_id | GSE31637
| | Sample_data_row_count | 45101
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