Search results for the GEO ID: GSE31646  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM785904 | GPL1261 |
|
Wild type, biological rep1
|
Epididymal fat, control
|
gender: Male
strain: C57BL/6
genotype/variation: B1+/+
tissue: Epididymal fat
age: 3 month-old
|
WT1
|
| Sample_geo_accession | GSM785904
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785904/suppl/GSM785904.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785905 | GPL1261 |
|
Wild type, biological rep2
|
Epididymal fat, control
|
gender: Male
strain: C57BL/6
genotype/variation: B1+/+
tissue: Epididymal fat
age: 3 month-old
|
WT2
|
| Sample_geo_accession | GSM785905
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785905/suppl/GSM785905.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785906 | GPL1261 |
|
Wild type, biological rep3
|
Epididymal fat, control
|
gender: Male
strain: C57BL/6
genotype/variation: B1+/+
tissue: Epididymal fat
age: 3 month-old
|
WT3
|
| Sample_geo_accession | GSM785906
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785906/suppl/GSM785906.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785907 | GPL1261 |
|
Wild type, biological rep4
|
Epididymal fat, control
|
gender: Male
strain: C57BL/6
genotype/variation: B1+/+
tissue: Epididymal fat
age: 3 month-old
|
WT4
|
| Sample_geo_accession | GSM785907
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785907/suppl/GSM785907.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785908 | GPL1261 |
|
Kinin B1 receptor knockout, biological rep1
|
Epididymal fat, knockout
|
gender: Male
strain: C57BL/6
genotype/variation: B1-/-
tissue: Epididymal fat
age: 3 month-old
|
KO1
|
| Sample_geo_accession | GSM785908
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785908/suppl/GSM785908.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785909 | GPL1261 |
|
Kinin B1 receptor knockout, biological rep2
|
Epididymal fat, knockout
|
gender: Male
strain: C57BL/6
genotype/variation: B1-/-
tissue: Epididymal fat
age: 3 month-old
|
KO2
|
| Sample_geo_accession | GSM785909
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785909/suppl/GSM785909.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785910 | GPL1261 |
|
Kinin B1 receptor knockout, biological rep3
|
Epididymal fat, knockout
|
gender: Male
strain: C57BL/6
genotype/variation: B1-/-
tissue: Epididymal fat
age: 3 month-old
|
KO3
|
| Sample_geo_accession | GSM785910
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785910/suppl/GSM785910.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785911 | GPL1261 |
|
Kinin B1 receptor knockout, biological rep4
|
Epididymal fat, knockout
|
gender: Male
strain: C57BL/6
genotype/variation: B1-/-
tissue: Epididymal fat
age: 3 month-old
|
KO4
|
| Sample_geo_accession | GSM785911
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785911/suppl/GSM785911.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785912 | GPL1261 |
|
Kinin B1 receptor knockout with selective rescue in fat, biological rep1
|
Epididymal fat, knockout+rescue
|
gender: Male
strain: C57BL/6
genotype/variation: aP2B1-B1-/-
tissue: Epididymal fat
age: 3 month-old
|
aP2B1KO1
|
| Sample_geo_accession | GSM785912
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785912/suppl/GSM785912.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785913 | GPL1261 |
|
Kinin B1 receptor knockout with selective rescue in fat, biological rep2
|
Epididymal fat, knockout+rescue
|
gender: Male
strain: C57BL/6
genotype/variation: aP2B1-B1-/-
tissue: Epididymal fat
age: 3 month-old
|
aP2B1KO2
|
| Sample_geo_accession | GSM785913
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785913/suppl/GSM785913.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
| | |
|
GSM785914 | GPL1261 |
|
Kinin B1 receptor knockout with selective rescue in fat, biological rep3
|
Epididymal fat, knockout+rescue
|
gender: Male
strain: C57BL/6
genotype/variation: aP2B1-B1-/-
tissue: Epididymal fat
age: 3 month-old
|
aP2B1KO3
|
| Sample_geo_accession | GSM785914
| | Sample_status | Public on Mar 31 2012
| | Sample_submission_date | Aug 24 2011
| | Sample_last_update_date | Mar 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Three-month-old mice were killed and tissues were collected and snap frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Animals were maintained on standard mouse chow diet at 22oC on 12 h light-dark cycle allowed ad libitum access to food and tap water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
| | Sample_hyb_protocol | 6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Marcelo,A.,Mori
| | Sample_contact_email | marcelo.mori@joslin.harvard.edu
| | Sample_contact_institute | Joslin Diabetes Center
| | Sample_contact_address | 1 Joslin Place
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM785nnn/GSM785914/suppl/GSM785914.CEL.gz
| | Sample_series_id | GSE31646
| | Sample_data_row_count | 45101
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