Search results for the GEO ID: GSE31864 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM790400 | GPL570 |
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EJ1
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Bladder cancer cell line
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cell line: EJ1
cell type: Human bladder carcinoma, fast growing tumour
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Sample_geo_accession | GSM790400
| Sample_status | Public on Sep 20 2011
| Sample_submission_date | Sep 02 2011
| Sample_last_update_date | Sep 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the MirVana kit (Ambion, TX) according to manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Affymetrix Human Genome U133 Plus 2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| Sample_platform_id | GPL570
| Sample_contact_name | Ewa,,Dudziec
| Sample_contact_email | mdp07ed@sheffield.ac.uk
| Sample_contact_department | ICS
| Sample_contact_institute | The University of Sheffield
| Sample_contact_address | Beech Hill Road
| Sample_contact_city | Sheffield
| Sample_contact_zip/postal_code | S10 2RX
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM790nnn/GSM790400/suppl/GSM790400_EJ1.CEL.gz
| Sample_series_id | GSE31864
| Sample_series_id | GSE31866
| Sample_data_row_count | 54675
| |
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GSM790401 | GPL570 |
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NHU
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Normal human urothelial cell line
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cell line: NHU
cell type: Normal human urothelial cell line, moderate growth
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Sample_geo_accession | GSM790401
| Sample_status | Public on Sep 20 2011
| Sample_submission_date | Sep 02 2011
| Sample_last_update_date | Sep 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the MirVana kit (Ambion, TX) according to manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Affymetrix Human Genome U133 Plus 2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| Sample_platform_id | GPL570
| Sample_contact_name | Ewa,,Dudziec
| Sample_contact_email | mdp07ed@sheffield.ac.uk
| Sample_contact_department | ICS
| Sample_contact_institute | The University of Sheffield
| Sample_contact_address | Beech Hill Road
| Sample_contact_city | Sheffield
| Sample_contact_zip/postal_code | S10 2RX
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM790nnn/GSM790401/suppl/GSM790401_NHU.CEL.gz
| Sample_series_id | GSE31864
| Sample_series_id | GSE31866
| Sample_data_row_count | 54675
| |
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GSM790402 | GPL570 |
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RT112
|
Bladder cancer cell line
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cell line: RT112
cell type: Human bladder carcinoma, relatively fast growing tumour
|
|
Sample_geo_accession | GSM790402
| Sample_status | Public on Sep 20 2011
| Sample_submission_date | Sep 02 2011
| Sample_last_update_date | Sep 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using the MirVana kit (Ambion, TX) according to manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Affymetrix Human Genome U133 Plus 2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| Sample_platform_id | GPL570
| Sample_contact_name | Ewa,,Dudziec
| Sample_contact_email | mdp07ed@sheffield.ac.uk
| Sample_contact_department | ICS
| Sample_contact_institute | The University of Sheffield
| Sample_contact_address | Beech Hill Road
| Sample_contact_city | Sheffield
| Sample_contact_zip/postal_code | S10 2RX
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM790nnn/GSM790402/suppl/GSM790402_RT112.CEL.gz
| Sample_series_id | GSE31864
| Sample_series_id | GSE31866
| Sample_data_row_count | 54675
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