Search results for the GEO ID: GSE31945  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM791677 | GPL1261 |
|
flow sorted cells from WT E12.5 1
|
flow sorted cells from WT E12.5 1
|
genotype: Lbx1-EGFP+|Pitx2+/+
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Wild Type 1
|
| Sample_geo_accession | GSM791677
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791677/suppl/GSM791677.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791677/suppl/GSM791677.CHP.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791678 | GPL1261 |
|
flow sorted cells from WT E12.5 2
|
flow sorted cells from WT E12.5 2
|
genotype: Lbx1-EGFP+|Pitx2+/+
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Wild Type 2
|
| Sample_geo_accession | GSM791678
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791678/suppl/GSM791678.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791678/suppl/GSM791678.CHP.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791679 | GPL1261 |
|
flow sorted cells from WT E12.5 3
|
flow sorted cells from WT E12.5 3
|
genotype: Lbx1-EGFP+|Pitx2+/+
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Wild Type 3
|
| Sample_geo_accession | GSM791679
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791679/suppl/GSM791679.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791679/suppl/GSM791679.chp.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791680 | GPL1261 |
|
flow sorted cells from HET E12.5 1
|
flow sorted cells from HET E12.5 1
|
genotype: Lbx1-EGFP+|Pitx2-LacZ/+
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Heterozygote 1
|
| Sample_geo_accession | GSM791680
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791680/suppl/GSM791680.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791680/suppl/GSM791680.CHP.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791681 | GPL1261 |
|
flow sorted cells from HET E12.5 2
|
flow sorted cells from HET E12.5 2
|
genotype: Lbx1-EGFP+|Pitx2-LacZ/+
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Heterozygote 2
|
| Sample_geo_accession | GSM791681
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791681/suppl/GSM791681.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791681/suppl/GSM791681.CHP.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791682 | GPL1261 |
|
flow sorted cells from HET E12.5 3
|
flow sorted cells from HET E12.5 3
|
genotype: Lbx1-EGFP+|Pitx2-LacZ/+
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Heterozygote 3
|
| Sample_geo_accession | GSM791682
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791682/suppl/GSM791682.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791682/suppl/GSM791682.chp.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791683 | GPL1261 |
|
flow sorted cells form MUT E12.5 1
|
flow sorted cells form MUT E12.5 1
|
genotype: Lbx1-EGFP+|Pitx2-LacZ/LacZ
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Mutant 1
|
| Sample_geo_accession | GSM791683
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791683/suppl/GSM791683.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791683/suppl/GSM791683.CHP.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791684 | GPL1261 |
|
flow sorted cells form MUT E12.5 2
|
flow sorted cells form MUT E12.5 2
|
genotype: Lbx1-EGFP+|Pitx2-LacZ/LacZ
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Mutant 2
|
| Sample_geo_accession | GSM791684
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791684/suppl/GSM791684.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791684/suppl/GSM791684.CHP.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
GSM791685 | GPL1261 |
|
flow sorted cells form MUT E12.5 3
|
flow sorted cells form MUT E12.5 3
|
genotype: Lbx1-EGFP+|Pitx2-LacZ/LacZ
age: E12.5
tissue: Flow sorted forelimb tissue
|
Pitx2 Mutant 3
|
| Sample_geo_accession | GSM791685
| | Sample_status | Public on Jun 11 2013
| | Sample_submission_date | Sep 06 2011
| | Sample_last_update_date | Jun 11 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Tissue was dissociated into single cells by enzymatic digestion with collagenase type 1, passed through 30 um filter and flow sorted on basis of GFP+
| | Sample_growth_protocol_ch1 | Forelimb tissue were dissected from genotyped E12.5 embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using protocol from Qiagen Rneasy Mini Kit Handbook supplied by Manufacturer
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 200 ng total RNA was labeled with Biotin using Ambion MessaAMP premier kit, using manufacturers instructions
| | Sample_hyb_protocol | 10 µg biotin labeled cRNA along with Affymetrix eukaryotic hybridization controls were hybridized for 16 hours in an Affymetrix GeneChip Hybridization Oven 640 at 45C. Washing, staining with phycoerithryn-streptavidin follow the GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays (P/N 702731) protocols using AGCC software (possibly GCOS for older than fall 2009) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_scan_protocol | scanning followed the Affymetrix GeneChip® Expression Analysis Technical Manual (701021 Rev. 5) with a Affymetrix GeneChip® Fluidics Station and a GeneChip® Scanner 3000 with autoloader.
| | Sample_data_processing | Data was analysed using Filemaker Pro software and RMA normalization. See Kioussi and Gross PLoS One 2008 [PMID: 18769640] and Kioussi et al., PNAS 2006 [PMID: 17132738] for further details
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Adam,Lee,Campbell
| | Sample_contact_email | campbada@onid.orst.edu
| | Sample_contact_phone | 541-737-8317
| | Sample_contact_laboratory | Dr. Chrissa Kioussi
| | Sample_contact_department | College of Pharmacy
| | Sample_contact_institute | Oregon State University
| | Sample_contact_address | 301 Pharmacy Building
| | Sample_contact_city | Corvallis
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97331
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791685/suppl/GSM791685.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM791nnn/GSM791685/suppl/GSM791685.chp.gz
| | Sample_series_id | GSE31945
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
