Search results for the GEO ID: GSE32057 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM794765 | GPL570 |
|
SDS 1
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 3
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794765
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794765/suppl/GSM794765_S1.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794766 | GPL570 |
|
SDS 2
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 19.4
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794766
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794766/suppl/GSM794766_S2.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794767 | GPL570 |
|
SDS 3
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 20
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794767
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794767/suppl/GSM794767_S3.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794768 | GPL570 |
|
SDS 4
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 22
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794768
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794768/suppl/GSM794768_S4.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794769 | GPL570 |
|
SDS 5
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 8.2
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794769
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794769/suppl/GSM794769_S5.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794770 | GPL570 |
|
SDS 6
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 24
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794770
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794770/suppl/GSM794770_S6.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794771 | GPL570 |
|
SDS 7
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 32.8
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794771
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794771/suppl/GSM794771_S7.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794772 | GPL570 |
|
SDS 8
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 11.2
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794772
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794772/suppl/GSM794772_S8.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794773 | GPL570 |
|
SDS 9
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS mutant
age (y): 14.2
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794773
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794773/suppl/GSM794773_S9.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794774 | GPL570 |
|
Normal 1
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): unknown
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794774
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794774/suppl/GSM794774_N1.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794775 | GPL570 |
|
Normal 2
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): 17
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794775
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Nov 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794775/suppl/GSM794775_N2.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794776 | GPL570 |
|
Normal 3
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): 17
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794776
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Nov 09 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794776/suppl/GSM794776_N3.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794778 | GPL570 |
|
Normal 5
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): 12.5
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794778
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794778/suppl/GSM794778_N5.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794779 | GPL570 |
|
Normal 6
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): 5
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794779
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794779/suppl/GSM794779_N6.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794780 | GPL570 |
|
Normal 7
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): 4
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794780
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794780/suppl/GSM794780_N7.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
GSM794782 | GPL570 |
|
Normal 9
|
Bone marrow mononuclear cells
|
cell type: Bone marrow mononuclear cells
genotype: SBDS Positive
age (y): 5
disease state: Shwachman-Diamond syndrome (SDS)
|
Gene expression data from bone marrow mononuclear cells.
Fresh bone marrow samples were obtained from nine patients with SDS. Bone marrow samples were collected into syringes containing preservative-free heparin. The bone marrow mononuclear cell fraction was separated using Ficoll-Hypaque density gradient centrifugation.
|
Sample_geo_accession | GSM794782
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 12 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized according to the manufacturers instructions on GeneChip U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip4 Scanner 3000 (Affymetrix Inc.)
| Sample_data_processing | Gene expression signal, detection call, signal log ratio and change call were calculated using GeneChip Operating Software by the statistical algorithm published previously by Affymetrix (Hubbell et al, 2002; Liu et al, 2002)
| Sample_platform_id | GPL570
| Sample_contact_name | Saswati,,Sen
| Sample_contact_email | saswati.sen@utoronto.ca
| Sample_contact_institute | Hospital for Sick Children
| Sample_contact_address | 555 university avenue
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM794nnn/GSM794782/suppl/GSM794782_N9.CEL.gz
| Sample_series_id | GSE32057
| Sample_data_row_count | 54675
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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