Search results for the GEO ID: GSE32078 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM795342 | GPL1261 |
|
Embryonic day 13.5 control replicate 1
|
Heart tissue
|
age: E13.5
strain: Swiss Albino
disease status: control
|
Gene expression data from heart tissue isolated from embryonic day 13.5 control mouse
|
Sample_geo_accession | GSM795342
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795342/suppl/GSM795342.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795342/suppl/GSM795342.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795343 | GPL1261 |
|
Embryonic day 13.5 control replicate 2
|
Heart tissue
|
age: E13.5
strain: Swiss Albino
disease status: control
|
Gene expression data from heart tissue isolated from embryonic day 13.5 control mouse
|
Sample_geo_accession | GSM795343
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795343/suppl/GSM795343.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795343/suppl/GSM795343.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795344 | GPL1261 |
|
Embryonic day 13.5 control replicate 3
|
Heart tissue
|
age: E13.5
strain: Swiss Albino
disease status: control
|
Gene expression data from heart tissue isolated from embryonic day 13.5 control mouse
|
Sample_geo_accession | GSM795344
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795344/suppl/GSM795344.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795344/suppl/GSM795344.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795345 | GPL1261 |
|
Embryonic day 13.5 diabetes replicate 1
|
Heart tissue
|
age: E13.5
strain: Swiss Albino
disease status: diabetes
|
Gene expression data from heart tissue isolated from embryonic day 13.5 diabetes exposed mouse
|
Sample_geo_accession | GSM795345
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795345/suppl/GSM795345.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795345/suppl/GSM795345.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795346 | GPL1261 |
|
Embryonic day 13.5 diabetes replicate 2
|
Heart tissue
|
age: E13.5
strain: Swiss Albino
disease status: diabetes
|
Gene expression data from heart tissue isolated from embryonic day 13.5 diabetes exposed mouse
|
Sample_geo_accession | GSM795346
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795346/suppl/GSM795346.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795346/suppl/GSM795346.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795347 | GPL1261 |
|
Embryonic day 13.5 diabetes replicate 3
|
Heart tissue
|
age: E13.5
strain: Swiss Albino
disease status: diabetes
|
Gene expression data from heart tissue isolated from embryonic day 13.5 diabetes exposed mouse
|
Sample_geo_accession | GSM795347
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795347/suppl/GSM795347.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795347/suppl/GSM795347.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795348 | GPL1261 |
|
Embryonic day 15.5 control replicate 1
|
Heart tissue
|
age: E15.5
strain: Swiss Albino
disease status: control
|
Gene expression data from heart tissue isolated from embryonic day 15.5 control mouse
|
Sample_geo_accession | GSM795348
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795348/suppl/GSM795348.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795348/suppl/GSM795348.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795349 | GPL1261 |
|
Embryonic day 15.5 control replicate 2
|
Heart tissue
|
age: E15.5
strain: Swiss Albino
disease status: control
|
Gene expression data from heart tissue isolated from embryonic day 15.5 control mouse
|
Sample_geo_accession | GSM795349
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795349/suppl/GSM795349.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795349/suppl/GSM795349.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795350 | GPL1261 |
|
Embryonic day 15.5 control replicate 3
|
Heart tissue
|
age: E15.5
strain: Swiss Albino
disease status: control
|
Gene expression data from heart tissue isolated from embryonic day 15.5 control mouse
|
Sample_geo_accession | GSM795350
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795350/suppl/GSM795350.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795350/suppl/GSM795350.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795351 | GPL1261 |
|
Embryonic day 15.5 diabetes replicate 1
|
Heart tissue
|
age: E15.5
strain: Swiss Albino
disease status: diabetes
|
Gene expression data from heart tissue isolated from embryonic day 15.5 diabetes exposed mouse
|
Sample_geo_accession | GSM795351
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795351/suppl/GSM795351.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795351/suppl/GSM795351.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795352 | GPL1261 |
|
Embryonic day 15.5 diabetes replicate 2
|
Heart tissue
|
age: E15.5
strain: Swiss Albino
disease status: diabetes
|
Gene expression data from heart tissue isolated from embryonic day 15.5 diabetes exposed mouse
|
Sample_geo_accession | GSM795352
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795352/suppl/GSM795352.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795352/suppl/GSM795352.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
GSM795353 | GPL1261 |
|
Embryonic day 15.5 diabetes replicate 3
|
Heart tissue
|
age: E15.5
strain: Swiss Albino
disease status: diabetes
|
Gene expression data from heart tissue isolated from embryonic day 15.5 diabetes exposed mouse
|
Sample_geo_accession | GSM795353
| Sample_status | Public on Sep 14 2011
| Sample_submission_date | Sep 13 2011
| Sample_last_update_date | Sep 14 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes mellitus was induced in 8 week-old female mice by an intraperitoneal injection of streptozotocin (STZ, 75mg/kg body weight, Sigma, USA) dissolved in citrate buffer (0.01 M, pH 4.5) for three successive days. Blood glucose level was examined one week after STZ injection using Glucometer Elite (Bayer, USA). Mice with non-fasting blood glucose level exceeding 16 mmol/l were used as experimental diabetic mice. In contrast, control mice maintained the normal blood glucose levels (4– 6 mmol/l) before and during pregnancy. Timed mating was carried out by placing four female mice with one normal male mouse in a cage overnight. The day when a copulation plug was observed was counted as embryonic day 0.5 (E0.5). Time-mated pregnant mice were divided into two groups control and diabetes. At E13.5 and E15.5, pregnant mice were anaesthetized with pentobarbital (150 mg/kg body wt, intraperitoneally) and embryos were collected after Caesarean. For each experiment, hearts were isolated from embryos from the two groups at different time points. The developmental stage, E13.5 corresponds to ~6 weeks of human gestation, hence it was chosen for this study. At this stage all 4 chambers of the heart were clearly distinguishable, and the outflow tract was evident. In addition, the heart phenotype was apparent in embryos from diabetic mice. The embryos with cardiac malformations from diabetic mice were classified as diabetic group and normal embryos from diabetic and non-diabetic mice were used as control groups.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from developing hearts was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA samples were processed according to the Affymetrix 3’ IVT Express protocol and Origen Lab’s SOP for 3’ IVT Express analysis. Briefly, 100 ng of total RNA was reverse transcribed to produce cDNA, which was subsequently used as a template to create biotin- labeled aRNA (amplified RNA).
| Sample_hyb_protocol | The labeled cRNA was then cleaned up, fragmented, and hybridized to Mouse Genome 430 2.0 Array (Affymetrix, CA, USA).
| Sample_scan_protocol | Arrays were using an Affymetrix 3000 7G scanner.
| Sample_data_processing | CEL files were exported to Affymetrix Expression Console Version 1.1 and RMA normalization (using EC1.1) was carried out.
| Sample_platform_id | GPL1261
| Sample_contact_name | Srinivasan,,Dinesh Kumar
| Sample_contact_email | dineshkumar@ntu.edu.sg
| Sample_contact_phone | +65-65923055
| Sample_contact_fax | +65-67908140
| Sample_contact_department | Lee Kong Chian School of Medicine
| Sample_contact_institute | Nanyang Technological University
| Sample_contact_address | 50 Nanyang Drive, Research Techno Plaza
| Sample_contact_city | Singapore
| Sample_contact_zip/postal_code | 637553
| Sample_contact_country | Singapore
| Sample_contact_web_link | http://scholar.google.com/citations?hl=en&user=FgC87RUAAAAJ
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795353/suppl/GSM795353.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795353/suppl/GSM795353.CHP.gz
| Sample_series_id | GSE32078
| Sample_data_row_count | 45101
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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