Result

Search results for the GEO ID: GSE32081

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM795456

GPL1261

ESC_SK7

ES cell line SK7

gender: male strain: ICR cell type: ES cell

ES cell line SK7

Sample_geo_accession	GSM795456
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795456/suppl/GSM795456_ESC_SK7.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

GSM795457

GPL1261

Ectoderm_SK7

Ectoderm derived from SK7

gender: male strain: ICR cell type: Ectoderm

Ectoderm derived from SK7

Sample_geo_accession	GSM795457
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795457/suppl/GSM795457_Ectoderm_SK7.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

GSM795458

GPL1261

Endoderm_SK7

Endoderm derived from SK7

gender: male strain: ICR cell type: Endoderm

Endoderm derived from SK7

Sample_geo_accession	GSM795458
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795458/suppl/GSM795458_Endoderm_SK7.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

GSM795459

GPL1261

Paraxial_mesoderm_SK7

Paraxial mesoderm derived from SK7

gender: male strain: ICR cell type: Paraxial Mesoderm

Paraxial mesoderm derived from SK7

Sample_geo_accession	GSM795459
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795459/suppl/GSM795459_Paraxial_mesoderm_SK7.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

GSM795460

GPL1261

Brain_ICR

Brain from ICR strain mouse

gender: male strain: ICR cell type: Brain

Brain from ICR strain mouse

Sample_geo_accession	GSM795460
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795460/suppl/GSM795460_Brain_ICR.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

GSM795461

GPL1261

Liver_ICR

Liver from ICR strain mouse

gender: male strain: ICR cell type: Liver

Liver from ICR strain mouse

Sample_geo_accession	GSM795461
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795461/suppl/GSM795461_Liver_ICR.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

GSM795462

GPL1261

Sk_muscle_ICR

Skeletal muscle from ICR strain mouse

gender: male strain: ICR cell type: Sleletal muscle

Skeletal muscle from ICR strain mouse

Sample_geo_accession	GSM795462
Sample_status	Public on Feb 15 2012
Sample_submission_date	Sep 13 2011
Sample_last_update_date	Feb 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with an RNeasy Mini Kit (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Affymetrix). Double-stranded cDNA was cleaned up by using Phase Lock Gels (Eppendorf)-Phenol/Chloroform Extraction (Ambion). In vitro transcription was performed on all of cDNA using the GeneChip IVT Labeling Kit (Affymetrix). The cRNA was cleaned up by using RNeasy Mini spin column (QIAGEN). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridized (45 degree Celsius, 16 hours). Hybridization was controled by adding cRNA cocktail (BioC, BioD, Cre) mixed as described in previous versions of the Expression Analysis Technical Manual. Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v5.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Chip analysis was performed using the Affymetrix GeneChip Operating Software v1.3.
Sample_platform_id	GPL1261
Sample_contact_name	Takayuki,,Isagawa
Sample_contact_email	isagawa@genome.rcast.u-tokyo.ac.jp
Sample_contact_laboratory	Genome Science
Sample_contact_department	RCAST
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Komaba, Meguro-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	153-8904
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM795nnn/GSM795462/suppl/GSM795462_Sk_muscle_ICR.CEL.gz
Sample_series_id	GSE32081
Sample_series_id	GSE32082
Sample_data_row_count	45101

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