Search results for the GEO ID: GSE32108  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM796020 | GPL570 |
|
Control siRNA rep1
|
Control siRNA transfected HeLa S3 cell
|
transfection: Control siRNA
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796020
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796020/suppl/GSM796020_siRNA_Negative_control-1.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796021 | GPL570 |
|
Control siRNA rep2
|
Control siRNA transfected HeLa S3 cell
|
transfection: Control siRNA
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796021
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796021/suppl/GSM796021_siRNA_Negative_control-2.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796022 | GPL570 |
|
Control siRNA rep3
|
Control siRNA transfected HeLa S3 cell
|
transfection: Control siRNA
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796022
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796022/suppl/GSM796022_siRNA_Negative_control-3.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796023 | GPL570 |
|
Against CDK8-1 rep1
|
Against CDK8-1 transfected HeLa S3 cell
|
transfection: CDK8-1
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796023
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796023/suppl/GSM796023_CDK8-1-1.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796024 | GPL570 |
|
Against CDK8-1 rep2
|
Against CDK8-1 transfected HeLa S3 cell
|
transfection: CDK8-1
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796024
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796024/suppl/GSM796024_CDK8-1-2.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796025 | GPL570 |
|
Against CDK8-1 rep3
|
Against CDK8-1 transfected HeLa S3 cell
|
transfection: CDK8-1
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796025
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796025/suppl/GSM796025_CDK8-1-3.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796026 | GPL570 |
|
Against CDK8-2 rep1
|
Against CDK8-2 transfected HeLa S3 cell
|
transfection: CDK8-2
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796026
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796026/suppl/GSM796026_CDK8-2-1.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796027 | GPL570 |
|
Against CDK8-2 rep2
|
Against CDK8-2 transfected HeLa S3 cell
|
transfection: CDK8-2
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796027
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796027/suppl/GSM796027_CDK8-2-2.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796028 | GPL570 |
|
Against CDK8-2 rep3
|
Against CDK8-2 transfected HeLa S3 cell
|
transfection: CDK8-2
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796028
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796028/suppl/GSM796028_CDK8-2-3.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796029 | GPL570 |
|
Against CDK19-1 rep1
|
Against CDK19-1 transfected HeLa S3 cell
|
transfection: CDK19-1
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796029
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796029/suppl/GSM796029_CDK19-1-1.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796030 | GPL570 |
|
Against CDK19-1 rep2
|
Against CDK19-1 transfected HeLa S3 cell
|
transfection: CDK19-1
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796030
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796030/suppl/GSM796030_CDK19-1-2.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796031 | GPL570 |
|
Against CDK19-1 rep3
|
Against CDK19-1 transfected HeLa S3 cell
|
transfection: CDK19-1
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796031
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796031/suppl/GSM796031_CDK19-1-3.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796032 | GPL570 |
|
Against CDK19-2 rep1
|
Against CDK19-2 transfected HeLa S3 cell
|
transfection: CDK19-2
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796032
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796032/suppl/GSM796032_CDK19-2-1.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796033 | GPL570 |
|
Against CDK19-2 rep2
|
Against CDK19-2 transfected HeLa S3 cell
|
transfection: CDK19-2
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796033
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796033/suppl/GSM796033_CDK19-2-2.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
|
GSM796034 | GPL570 |
|
Against CDK19-2 rep3
|
Against CDK19-2 transfected HeLa S3 cell
|
transfection: CDK19-2
cell line: HeLa S3
|
|
| Sample_geo_accession | GSM796034
| | Sample_status | Public on Sep 15 2011
| | Sample_submission_date | Sep 14 2011
| | Sample_last_update_date | Sep 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | We transfect siRNA using Lipofectamine 2000 (invitrogen) according to its manual and siRNA final concentration in the medium was 20nM. After culturing for 48hr, transfection medium was changed to fresh antibiotic containing medium. Then the cells were incubated for additional 12hr
| | Sample_growth_protocol_ch1 | HeLa S3 cells were seeded on 6-well plate at 4 x 104 cells per well in 2ml antibiotic free DMEM medium before 24hr transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared using RNeazy column (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with RMA using GeneSpring software. Base line was created as median of all sample.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Taiki,,Tsutsui
| | Sample_contact_email | d0961204@ems.u-toyama.ac.jp
| | Sample_contact_institute | Univ. Toyama
| | Sample_contact_address | sugitani2630
| | Sample_contact_city | Toyama
| | Sample_contact_zip/postal_code | 9300194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM796nnn/GSM796034/suppl/GSM796034_CDK19-2-3.CEL.gz
| | Sample_series_id | GSE32108
| | Sample_data_row_count | 54675
| | |
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