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Search results for the GEO ID: GSE32265

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM799444

GPL1261

Raptor Wildtype rep1

Raptor Wildtype

strain background: c57bl/6 cell type: Sorted HSPC 5 weeks post pIpC genotype/variation: Raptor Wildtype

WT1

Sample_geo_accession	GSM799444
Sample_status	Public on Sep 19 2012
Sample_submission_date	Sep 20 2011
Sample_last_update_date	Sep 19 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were treated with 15 micrograms/g body weight polyinosinic-polycytidylic acid (pIpC) three times once daily over 5 days, cells were sorted 5 weeks after the last injection
Sample_growth_protocol_ch1	In vivo
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted using the Arcturus® PicoPure® RNA isolation Kit according to the manufacturer’s recommendations (Applied Biosystems, Carlsbad, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was amplified with the Ovation® Pico WTA System (Nugen, San Carlos, CA), and fragmented and biotinylated with the Encore™ Biotin Module (Nugen)
Sample_hyb_protocol	Labeled cDNA was hybridized to the Affymetrics 430 2.0 microarray using standard conditions.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
Sample_platform_id	GPL1261
Sample_contact_name	Amit,,Sinha
Sample_contact_email	amit.sinha@childrens.harvard.edu
Sample_contact_phone	617-582-7579
Sample_contact_laboratory	Armstrong Lab
Sample_contact_department	Pediatric Oncology
Sample_contact_institute	Dana-Farber Cancer Institute
Sample_contact_address	44 Binney St
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02135
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM799nnn/GSM799444/suppl/GSM799444_SA2011071447.CEL.gz
Sample_series_id	GSE32265
Sample_data_row_count	45101

GSM799445

GPL1261

Raptor Wildtype rep2

Raptor Wildtype

strain background: c57bl/6 cell type: Sorted HSPC 5 weeks post pIpC genotype/variation: Raptor Wildtype

WT2

Sample_geo_accession	GSM799445
Sample_status	Public on Sep 19 2012
Sample_submission_date	Sep 20 2011
Sample_last_update_date	Sep 19 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were treated with 15 micrograms/g body weight polyinosinic-polycytidylic acid (pIpC) three times once daily over 5 days, cells were sorted 5 weeks after the last injection
Sample_growth_protocol_ch1	In vivo
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted using the Arcturus® PicoPure® RNA isolation Kit according to the manufacturer’s recommendations (Applied Biosystems, Carlsbad, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was amplified with the Ovation® Pico WTA System (Nugen, San Carlos, CA), and fragmented and biotinylated with the Encore™ Biotin Module (Nugen)
Sample_hyb_protocol	Labeled cDNA was hybridized to the Affymetrics 430 2.0 microarray using standard conditions.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
Sample_platform_id	GPL1261
Sample_contact_name	Amit,,Sinha
Sample_contact_email	amit.sinha@childrens.harvard.edu
Sample_contact_phone	617-582-7579
Sample_contact_laboratory	Armstrong Lab
Sample_contact_department	Pediatric Oncology
Sample_contact_institute	Dana-Farber Cancer Institute
Sample_contact_address	44 Binney St
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02135
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM799nnn/GSM799445/suppl/GSM799445_SA2011071448.CEL.gz
Sample_series_id	GSE32265
Sample_data_row_count	45101

GSM799446

GPL1261

Raptor Wildtype rep3

Raptor Wildtype

strain background: c57bl/6 cell type: Sorted HSPC 5 weeks post pIpC genotype/variation: Raptor Wildtype

WT3

Sample_geo_accession	GSM799446
Sample_status	Public on Sep 19 2012
Sample_submission_date	Sep 20 2011
Sample_last_update_date	Sep 19 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were treated with 15 micrograms/g body weight polyinosinic-polycytidylic acid (pIpC) three times once daily over 5 days, cells were sorted 5 weeks after the last injection
Sample_growth_protocol_ch1	In vivo
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted using the Arcturus® PicoPure® RNA isolation Kit according to the manufacturer’s recommendations (Applied Biosystems, Carlsbad, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was amplified with the Ovation® Pico WTA System (Nugen, San Carlos, CA), and fragmented and biotinylated with the Encore™ Biotin Module (Nugen)
Sample_hyb_protocol	Labeled cDNA was hybridized to the Affymetrics 430 2.0 microarray using standard conditions.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
Sample_platform_id	GPL1261
Sample_contact_name	Amit,,Sinha
Sample_contact_email	amit.sinha@childrens.harvard.edu
Sample_contact_phone	617-582-7579
Sample_contact_laboratory	Armstrong Lab
Sample_contact_department	Pediatric Oncology
Sample_contact_institute	Dana-Farber Cancer Institute
Sample_contact_address	44 Binney St
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02135
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM799nnn/GSM799446/suppl/GSM799446_SA2011071449.CEL.gz
Sample_series_id	GSE32265
Sample_data_row_count	45101

GSM799447

GPL1261

Raptor Mutant (KO) rep1

Raptor Mutant (KO)

strain background: c57bl/6 cell type: Sorted HSPC 5 weeks post pIpC genotype/variation: Raptor Mutant (KO)

MT1

Sample_geo_accession	GSM799447
Sample_status	Public on Sep 19 2012
Sample_submission_date	Sep 20 2011
Sample_last_update_date	Sep 19 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were treated with 15 micrograms/g body weight polyinosinic-polycytidylic acid (pIpC) three times once daily over 5 days, cells were sorted 5 weeks after the last injection
Sample_growth_protocol_ch1	In vivo
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted using the Arcturus® PicoPure® RNA isolation Kit according to the manufacturer’s recommendations (Applied Biosystems, Carlsbad, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was amplified with the Ovation® Pico WTA System (Nugen, San Carlos, CA), and fragmented and biotinylated with the Encore™ Biotin Module (Nugen)
Sample_hyb_protocol	Labeled cDNA was hybridized to the Affymetrics 430 2.0 microarray using standard conditions.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
Sample_platform_id	GPL1261
Sample_contact_name	Amit,,Sinha
Sample_contact_email	amit.sinha@childrens.harvard.edu
Sample_contact_phone	617-582-7579
Sample_contact_laboratory	Armstrong Lab
Sample_contact_department	Pediatric Oncology
Sample_contact_institute	Dana-Farber Cancer Institute
Sample_contact_address	44 Binney St
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02135
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM799nnn/GSM799447/suppl/GSM799447_SA2011071450.CEL.gz
Sample_series_id	GSE32265
Sample_data_row_count	45101

GSM799448

GPL1261

Raptor Mutant (KO) rep2

Raptor Mutant (KO)

strain background: c57bl/6 cell type: Sorted HSPC 5 weeks post pIpC genotype/variation: Raptor Mutant (KO)

MT2

Sample_geo_accession	GSM799448
Sample_status	Public on Sep 19 2012
Sample_submission_date	Sep 20 2011
Sample_last_update_date	Sep 19 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were treated with 15 micrograms/g body weight polyinosinic-polycytidylic acid (pIpC) three times once daily over 5 days, cells were sorted 5 weeks after the last injection
Sample_growth_protocol_ch1	In vivo
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted using the Arcturus® PicoPure® RNA isolation Kit according to the manufacturer’s recommendations (Applied Biosystems, Carlsbad, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was amplified with the Ovation® Pico WTA System (Nugen, San Carlos, CA), and fragmented and biotinylated with the Encore™ Biotin Module (Nugen)
Sample_hyb_protocol	Labeled cDNA was hybridized to the Affymetrics 430 2.0 microarray using standard conditions.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
Sample_platform_id	GPL1261
Sample_contact_name	Amit,,Sinha
Sample_contact_email	amit.sinha@childrens.harvard.edu
Sample_contact_phone	617-582-7579
Sample_contact_laboratory	Armstrong Lab
Sample_contact_department	Pediatric Oncology
Sample_contact_institute	Dana-Farber Cancer Institute
Sample_contact_address	44 Binney St
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02135
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM799nnn/GSM799448/suppl/GSM799448_SA2011071451.CEL.gz
Sample_series_id	GSE32265
Sample_data_row_count	45101

GSM799449

GPL1261

Raptor Mutant (KO) rep3

Raptor Mutant (KO)

strain background: c57bl/6 cell type: Sorted HSPC 5 weeks post pIpC genotype/variation: Raptor Mutant (KO)

MT3

Sample_geo_accession	GSM799449
Sample_status	Public on Sep 19 2012
Sample_submission_date	Sep 20 2011
Sample_last_update_date	Sep 19 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were treated with 15 micrograms/g body weight polyinosinic-polycytidylic acid (pIpC) three times once daily over 5 days, cells were sorted 5 weeks after the last injection
Sample_growth_protocol_ch1	In vivo
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted using the Arcturus® PicoPure® RNA isolation Kit according to the manufacturer’s recommendations (Applied Biosystems, Carlsbad, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was amplified with the Ovation® Pico WTA System (Nugen, San Carlos, CA), and fragmented and biotinylated with the Encore™ Biotin Module (Nugen)
Sample_hyb_protocol	Labeled cDNA was hybridized to the Affymetrics 430 2.0 microarray using standard conditions.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
Sample_platform_id	GPL1261
Sample_contact_name	Amit,,Sinha
Sample_contact_email	amit.sinha@childrens.harvard.edu
Sample_contact_phone	617-582-7579
Sample_contact_laboratory	Armstrong Lab
Sample_contact_department	Pediatric Oncology
Sample_contact_institute	Dana-Farber Cancer Institute
Sample_contact_address	44 Binney St
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02135
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM799nnn/GSM799449/suppl/GSM799449_SA2011071452.CEL.gz
Sample_series_id	GSE32265
Sample_data_row_count	45101

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