Search results for the GEO ID: GSE32315  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM800588 | GPL1261 |
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T cells Stimulated
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T cells stimulated with anti-CD3/CD28 antibodies
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cell type: primary T cells
treatment: anti-CD3/CD28 antibodies
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| Sample_geo_accession | GSM800588
| | Sample_status | Public on Dec 29 2012
| | Sample_submission_date | Sep 22 2011
| | Sample_last_update_date | Dec 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3/CD28 antibodies were added to stimulate cells and triptolide(50ng/ml)were added to treat cells.
| | Sample_growth_protocol_ch1 | Purified T cells were cultured with fresh RPMI 1640 medium (Gibco, Grand Island, NY), supplemented with 20 % FBS, 2 mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 mg/ml). Cells were incubated in a 37 ◦C incubator supplied with 95 % air and 5 % CO2 for 18 h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Probes were biotin-labeled with a BioArray High Yield RNA Transcript Labeling Kit (Affymetrix, P/N 900182).
| | Sample_hyb_protocol | After labeling, the probes were purified, quantified and hybridized with Mouse Genome 430 2.0 Array chips (Affymetrix) for 16 h.
| | Sample_scan_protocol | The array chips were washed, stained and scanned with a G2500A GeneArray Scanner (Affymetrix).
| | Sample_data_processing | MAS 5
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zichun,,Hua
| | Sample_contact_email | zchua@nju.edu.cn
| | Sample_contact_phone | 86-25-83324605
| | Sample_contact_fax | 86-25-83324605
| | Sample_contact_institute | Nanjing University
| | Sample_contact_address | Hankou Road
| | Sample_contact_city | Nanjing
| | Sample_contact_state | Jiangsu
| | Sample_contact_zip/postal_code | 210093
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM800nnn/GSM800588/suppl/GSM800588_Stimulated.CEL.gz
| | Sample_series_id | GSE32315
| | Sample_data_row_count | 45037
| | |
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GSM800589 | GPL1261 |
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T cells stimulated and treated with triptolide
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T cells stimulated with anti-CD3/CD28 antibodies and treated with triptolide
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cell type: primary T cells
treatment: anti-CD3/CD28 antibodies and treated with triptolide
|
|
| Sample_geo_accession | GSM800589
| | Sample_status | Public on Dec 29 2012
| | Sample_submission_date | Sep 22 2011
| | Sample_last_update_date | Dec 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Anti-CD3/CD28 antibodies were added to stimulate cells and triptolide(50ng/ml)were added to treat cells.
| | Sample_growth_protocol_ch1 | Purified T cells were cultured with fresh RPMI 1640 medium (Gibco, Grand Island, NY), supplemented with 20 % FBS, 2 mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 mg/ml). Cells were incubated in a 37 ◦C incubator supplied with 95 % air and 5 % CO2 for 18 h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Probes were biotin-labeled with a BioArray High Yield RNA Transcript Labeling Kit (Affymetrix, P/N 900182).
| | Sample_hyb_protocol | After labeling, the probes were purified, quantified and hybridized with Mouse Genome 430 2.0 Array chips (Affymetrix) for 16 h.
| | Sample_scan_protocol | The array chips were washed, stained and scanned with a G2500A GeneArray Scanner (Affymetrix).
| | Sample_data_processing | MAS 5
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zichun,,Hua
| | Sample_contact_email | zchua@nju.edu.cn
| | Sample_contact_phone | 86-25-83324605
| | Sample_contact_fax | 86-25-83324605
| | Sample_contact_institute | Nanjing University
| | Sample_contact_address | Hankou Road
| | Sample_contact_city | Nanjing
| | Sample_contact_state | Jiangsu
| | Sample_contact_zip/postal_code | 210093
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM800nnn/GSM800589/suppl/GSM800589_Stimulated+Tp.CEL.gz
| | Sample_series_id | GSE32315
| | Sample_data_row_count | 45037
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