Search results for the GEO ID: GSE32461  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM803264 | GPL1261 |
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Sox2GFP+ckit- testis, biological rep 1
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Sox2GFP+ckit- Mouse testis cells
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age: 2 week old
strain: 129SvJae/BL6
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Gene expression data from mouse testis cells that are able to regenerate damaged testis tubules.
|
| Sample_geo_accession | GSM803264
| | Sample_status | Public on Oct 06 2011
| | Sample_submission_date | Sep 28 2011
| | Sample_last_update_date | Oct 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | 2 week old SoxGFP mice (129SvJae/BL6)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Mouse testes of 2-week old Sox2GFP mice were isolated, and dissociated with collagenase. Single cell suspensions were generated and stained with FACS antibody for ckit. Cells were FACS sorted into Trizol, RNA was purified from approximately 20.000-90.000 cells per specimen using the RNeasy Mini-kit (Qiagen, Hilden, Germany) following the manufacturer's recommended protocol utilizing DNase treatment.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA was prepared from 5 ng total RNA using the Ovation Pico WTA System and Encore Biotin Module (NuGEN, San Carlos, California).
| | Sample_hyb_protocol | Following fragmentation, amplification and labeling, 5 ug of cDNA was hybridized to the Affymetrix HG-U133A chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GS 3000 Scanner with G7 upgrade using factory PMT settings.
| | Sample_data_processing | ExpressionAnalysisR with RMA normalization using Genesifter/Geospiza
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Katrin,,Arnold
| | Sample_contact_email | karnold@helix.mgh.harvard.edu
| | Sample_contact_phone | 617-643-1986
| | Sample_contact_laboratory | Hochedlinger lab
| | Sample_contact_department | Cancer Center
| | Sample_contact_institute | MGH
| | Sample_contact_address | 185 Cambridge Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803264/suppl/GSM803264.CEL.gz
| | Sample_series_id | GSE32461
| | Sample_data_row_count | 45101
| | |
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GSM803265 | GPL1261 |
|
Sox2GFP+ckit- testis, biological rep 2
|
Sox2GFP+ckit- Mouse testis cells
|
age: 2 week old
strain: 129SvJae/BL6
|
Gene expression data from mouse testis cells that are able to regenerate damaged testis tubules.
|
| Sample_geo_accession | GSM803265
| | Sample_status | Public on Oct 06 2011
| | Sample_submission_date | Sep 28 2011
| | Sample_last_update_date | Oct 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | 2 week old SoxGFP mice (129SvJae/BL6)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Mouse testes of 2-week old Sox2GFP mice were isolated, and dissociated with collagenase. Single cell suspensions were generated and stained with FACS antibody for ckit. Cells were FACS sorted into Trizol, RNA was purified from approximately 20.000-90.000 cells per specimen using the RNeasy Mini-kit (Qiagen, Hilden, Germany) following the manufacturer's recommended protocol utilizing DNase treatment.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA was prepared from 5 ng total RNA using the Ovation Pico WTA System and Encore Biotin Module (NuGEN, San Carlos, California).
| | Sample_hyb_protocol | Following fragmentation, amplification and labeling, 5 ug of cDNA was hybridized to the Affymetrix HG-U133A chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GS 3000 Scanner with G7 upgrade using factory PMT settings.
| | Sample_data_processing | ExpressionAnalysisR with RMA normalization using Genesifter/Geospiza
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Katrin,,Arnold
| | Sample_contact_email | karnold@helix.mgh.harvard.edu
| | Sample_contact_phone | 617-643-1986
| | Sample_contact_laboratory | Hochedlinger lab
| | Sample_contact_department | Cancer Center
| | Sample_contact_institute | MGH
| | Sample_contact_address | 185 Cambridge Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803265/suppl/GSM803265.CEL.gz
| | Sample_series_id | GSE32461
| | Sample_data_row_count | 45101
| | |
|
GSM803266 | GPL1261 |
|
Sox2GFP+ckit+ testis, biological rep 1
|
Sox2GFP+ckit+ Mouse testis cells
|
age: 2 week old
strain: 129SvJae/BL6
|
Gene expression data from mouse testis cells that are NOT able to regenerate damaged testis tubules.
|
| Sample_geo_accession | GSM803266
| | Sample_status | Public on Oct 06 2011
| | Sample_submission_date | Sep 28 2011
| | Sample_last_update_date | Oct 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | 2 week old SoxGFP mice (129SvJae/BL6)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Mouse testes of 2-week old Sox2GFP mice were isolated, and dissociated with collagenase. Single cell suspensions were generated and stained with FACS antibody for ckit. Cells were FACS sorted into Trizol, RNA was purified from approximately 20.000-90.000 cells per specimen using the RNeasy Mini-kit (Qiagen, Hilden, Germany) following the manufacturer's recommended protocol utilizing DNase treatment.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA was prepared from 5 ng total RNA using the Ovation Pico WTA System and Encore Biotin Module (NuGEN, San Carlos, California).
| | Sample_hyb_protocol | Following fragmentation, amplification and labeling, 5 ug of cDNA was hybridized to the Affymetrix HG-U133A chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GS 3000 Scanner with G7 upgrade using factory PMT settings.
| | Sample_data_processing | ExpressionAnalysisR with RMA normalization using Genesifter/Geospiza
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Katrin,,Arnold
| | Sample_contact_email | karnold@helix.mgh.harvard.edu
| | Sample_contact_phone | 617-643-1986
| | Sample_contact_laboratory | Hochedlinger lab
| | Sample_contact_department | Cancer Center
| | Sample_contact_institute | MGH
| | Sample_contact_address | 185 Cambridge Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803266/suppl/GSM803266.CEL.gz
| | Sample_series_id | GSE32461
| | Sample_data_row_count | 45101
| | |
|
GSM803267 | GPL1261 |
|
Sox2GFP+ckit+ testis, biological rep 2
|
Sox2GFP+ckit+ Mouse testis cells
|
age: 2 week old
strain: 129SvJae/BL6
|
Gene expression data from mouse testis cells that are NOT able to regenerate damaged testis tubules.
|
| Sample_geo_accession | GSM803267
| | Sample_status | Public on Oct 06 2011
| | Sample_submission_date | Sep 28 2011
| | Sample_last_update_date | Oct 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | 2 week old SoxGFP mice (129SvJae/BL6)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Mouse testes of 2-week old Sox2GFP mice were isolated, and dissociated with collagenase. Single cell suspensions were generated and stained with FACS antibody for ckit. Cells were FACS sorted into Trizol, RNA was purified from approximately 20.000-90.000 cells per specimen using the RNeasy Mini-kit (Qiagen, Hilden, Germany) following the manufacturer's recommended protocol utilizing DNase treatment.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA was prepared from 5 ng total RNA using the Ovation Pico WTA System and Encore Biotin Module (NuGEN, San Carlos, California).
| | Sample_hyb_protocol | Following fragmentation, amplification and labeling, 5 ug of cDNA was hybridized to the Affymetrix HG-U133A chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GS 3000 Scanner with G7 upgrade using factory PMT settings.
| | Sample_data_processing | ExpressionAnalysisR with RMA normalization using Genesifter/Geospiza
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Katrin,,Arnold
| | Sample_contact_email | karnold@helix.mgh.harvard.edu
| | Sample_contact_phone | 617-643-1986
| | Sample_contact_laboratory | Hochedlinger lab
| | Sample_contact_department | Cancer Center
| | Sample_contact_institute | MGH
| | Sample_contact_address | 185 Cambridge Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02215
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803267/suppl/GSM803267.CEL.gz
| | Sample_series_id | GSE32461
| | Sample_data_row_count | 45101
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