Search results for the GEO ID: GSE32482  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM803789 | GPL570 |
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GB2-siCont
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Glioblastoma stem cell, Control shRNA
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cell line: GB2
cell type: Glioblastoma stem cell
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| Sample_geo_accession | GSM803789
| | Sample_status | Public on Oct 01 2011
| | Sample_submission_date | Sep 29 2011
| | Sample_last_update_date | Oct 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was extracted using the NucleoSpin RNA Clean-up kit (Macherey-Nagel) according to manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double-stranded cDNA was performed using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogene), and cleaned up with GeneChip Sample Cleanup Module (Affymetrix), the resulting products were then be used to synthesize biotin-labeled cRNA with Enzo Bioarray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences) and further fragmented to 35-200 bp oligos. All procedures were done according to manufacturer’s instruction.
| | Sample_hyb_protocol | Following fragmentation, 30 µl fragmented cRNA at the concentration of 500 ng/µl were hybridized for 16 hr at 45C on HG-U133 plus 2.0 GeneChips (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Data sets were imported into Gene Spring for normalization and statistical analysis.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tetsu,,Akiyama
| | Sample_contact_email | akiyama@iam.u-tokyo.ac.jp
| | Sample_contact_laboratory | Laboratory of Molecular and Genetic Information
| | Sample_contact_department | Institute of Molecular and Cellular Biosciences
| | Sample_contact_institute | Tokyo University
| | Sample_contact_address | 1-1-1, Yayoi, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 110-0032
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803789/suppl/GSM803789_110520_1_gbm_stem_cont_shRNA.CEL.gz
| | Sample_series_id | GSE32482
| | Sample_data_row_count | 54675
| | |
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GSM803806 | GPL570 |
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GB2-shALK
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Glioblastoma stem cell, shRNA targeting ALK
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cell line: GB2
cell type: Glioblastoma stem cell
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| Sample_geo_accession | GSM803806
| | Sample_status | Public on Oct 01 2011
| | Sample_submission_date | Sep 29 2011
| | Sample_last_update_date | Oct 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was extracted using the NucleoSpin RNA Clean-up kit (Macherey-Nagel) according to manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double-stranded cDNA was performed using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogene), and cleaned up with GeneChip Sample Cleanup Module (Affymetrix), the resulting products were then be used to synthesize biotin-labeled cRNA with Enzo Bioarray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences) and further fragmented to 35-200 bp oligos. All procedures were done according to manufacturer’s instruction.
| | Sample_hyb_protocol | Following fragmentation, 30 µl fragmented cRNA at the concentration of 500 ng/µl were hybridized for 16 hr at 45C on HG-U133 plus 2.0 GeneChips (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Data sets were imported into Gene Spring for normalization and statistical analysis.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tetsu,,Akiyama
| | Sample_contact_email | akiyama@iam.u-tokyo.ac.jp
| | Sample_contact_laboratory | Laboratory of Molecular and Genetic Information
| | Sample_contact_department | Institute of Molecular and Cellular Biosciences
| | Sample_contact_institute | Tokyo University
| | Sample_contact_address | 1-1-1, Yayoi, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 110-0032
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803806/suppl/GSM803806_110520_2_gbm_stem_ALK_shRNA.CEL.gz
| | Sample_series_id | GSE32482
| | Sample_data_row_count | 54675
| | |
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GSM803807 | GPL570 |
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GB2-shPTN
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Glioblastoma stem cell, shRNA targeting pleiotrophin
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cell line: GB2
cell type: Glioblastoma stem cell
|
|
| Sample_geo_accession | GSM803807
| | Sample_status | Public on Oct 01 2011
| | Sample_submission_date | Sep 29 2011
| | Sample_last_update_date | Oct 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was extracted using the NucleoSpin RNA Clean-up kit (Macherey-Nagel) according to manufacturer's instruction.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double-stranded cDNA was performed using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogene), and cleaned up with GeneChip Sample Cleanup Module (Affymetrix), the resulting products were then be used to synthesize biotin-labeled cRNA with Enzo Bioarray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences) and further fragmented to 35-200 bp oligos. All procedures were done according to manufacturer’s instruction.
| | Sample_hyb_protocol | Following fragmentation, 30 µl fragmented cRNA at the concentration of 500 ng/µl were hybridized for 16 hr at 45C on HG-U133 plus 2.0 GeneChips (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Data sets were imported into Gene Spring for normalization and statistical analysis.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tetsu,,Akiyama
| | Sample_contact_email | akiyama@iam.u-tokyo.ac.jp
| | Sample_contact_laboratory | Laboratory of Molecular and Genetic Information
| | Sample_contact_department | Institute of Molecular and Cellular Biosciences
| | Sample_contact_institute | Tokyo University
| | Sample_contact_address | 1-1-1, Yayoi, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 110-0032
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM803nnn/GSM803807/suppl/GSM803807_110520_3_gbm_stem_ptn_shRNA.CEL.gz
| | Sample_series_id | GSE32482
| | Sample_data_row_count | 54675
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