Search results for the GEO ID: GSE32496 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM804399 | GPL570 |
|
A549 C1
|
NSCLC cell line A549
|
cell line: NSCLC cell line A549
treatment: untreated
|
untreated
|
Sample_geo_accession | GSM804399
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804399/suppl/GSM804399.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804400 | GPL570 |
|
A549 C2
|
NSCLC cell line A549
|
cell line: NSCLC cell line A549
treatment: untreated
|
untreated
|
Sample_geo_accession | GSM804400
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804400/suppl/GSM804400.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804401 | GPL570 |
|
A549 A1
|
NSCLC cell line A549
|
cell line: NSCLC cell line A549
treatment: Aza-dC treated
|
Aza-dC treated
|
Sample_geo_accession | GSM804401
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804401/suppl/GSM804401.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804402 | GPL570 |
|
A549 A2
|
NSCLC cell line A549
|
cell line: NSCLC cell line A549
treatment: Aza-dC treated
|
Aza-dC treated
|
Sample_geo_accession | GSM804402
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804402/suppl/GSM804402.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804403 | GPL570 |
|
A549 AT1
|
NSCLC cell line A549
|
cell line: NSCLC cell line A549
treatment: Aza-dC/TSA treated
|
Aza-dC/TSA treated
|
Sample_geo_accession | GSM804403
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804403/suppl/GSM804403.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804404 | GPL570 |
|
A549 AT2
|
NSCLC cell line A549
|
cell line: NSCLC cell line A549
treatment: Aza-dC/TSA treated
|
Aza-dC/TSA treated
|
Sample_geo_accession | GSM804404
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804404/suppl/GSM804404.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804405 | GPL570 |
|
H1993 C1
|
NSCLC cell line H1993
|
cell line: NSCLC cell line H1993
treatment: untreated
|
untreated
|
Sample_geo_accession | GSM804405
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804405/suppl/GSM804405.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804406 | GPL570 |
|
H1993 C2
|
NSCLC cell line H1993
|
cell line: NSCLC cell line H1993
treatment: untreated
|
untreated
|
Sample_geo_accession | GSM804406
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804406/suppl/GSM804406.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804407 | GPL570 |
|
H1993 A1
|
NSCLC cell line H1993
|
cell line: NSCLC cell line H1993
treatment: Aza-dC treated
|
Aza-dC treated
|
Sample_geo_accession | GSM804407
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804407/suppl/GSM804407.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804408 | GPL570 |
|
H1993 A2
|
NSCLC cell line H1993
|
cell line: NSCLC cell line H1993
treatment: Aza-dC treated
|
Aza-dC treated
|
Sample_geo_accession | GSM804408
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804408/suppl/GSM804408.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804409 | GPL570 |
|
H1993 AT1
|
NSCLC cell line H1993
|
cell line: NSCLC cell line H1993
treatment: Aza-dC/TSA treated
|
Aza-dC/TSA treated
|
Sample_geo_accession | GSM804409
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804409/suppl/GSM804409.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804410 | GPL570 |
|
H1993 AT2
|
NSCLC cell line H1993
|
cell line: NSCLC cell line H1993
treatment: Aza-dC/TSA treated
|
Aza-dC/TSA treated
|
Sample_geo_accession | GSM804410
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804410/suppl/GSM804410.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804411 | GPL570 |
|
H2073 C1
|
NSCLC cell line H2073
|
cell line: NSCLC cell line H2073
treatment: untreated
|
untreated
|
Sample_geo_accession | GSM804411
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804411/suppl/GSM804411.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804412 | GPL570 |
|
H2073 C2
|
NSCLC cell line H2073
|
cell line: NSCLC cell line H2073
treatment: untreated
|
untreated
|
Sample_geo_accession | GSM804412
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804412/suppl/GSM804412.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804413 | GPL570 |
|
H2073 A1
|
NSCLC cell line H2073
|
cell line: NSCLC cell line H2073
treatment: Aza-dC treated
|
Aza-dC treated
|
Sample_geo_accession | GSM804413
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804413/suppl/GSM804413.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804414 | GPL570 |
|
H2073 A2
|
NSCLC cell line H2073
|
cell line: NSCLC cell line H2073
treatment: Aza-dC treated
|
Aza-dC treated
|
Sample_geo_accession | GSM804414
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804414/suppl/GSM804414.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804415 | GPL570 |
|
H2073 AT1
|
NSCLC cell line H2073
|
cell line: NSCLC cell line H2073
treatment: Aza-dC/TSA treated
|
Aza-dC/TSA treated
|
Sample_geo_accession | GSM804415
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804415/suppl/GSM804415.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
GSM804416 | GPL570 |
|
H2073 AT2
|
NSCLC cell line H2073
|
cell line: NSCLC cell line H2073
treatment: Aza-dC/TSA treated
|
Aza-dC/TSA treated
|
Sample_geo_accession | GSM804416
| Sample_status | Public on Nov 26 2012
| Sample_submission_date | Sep 29 2011
| Sample_last_update_date | Nov 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 2x10^5 A549 cells/ml were treated either with 0.5 µM Aza-dC for 6 days or with the combination of 0.5 µM Aza-dC for 6 days and 100 ng/ml TSA for additional 24 hours.
| Sample_growth_protocol_ch1 | Cells were cultured using RPMI1640 supplemented with 10% FCS and incubated at 37 °C and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated using Trizol followed by clean up using Qiagen's RNAeasy mini spin colomn according to manufacturer's instuctions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| Sample_hyb_protocol | Each sample was hybridized to Affymetrix HG-U133_plus_2.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
| Sample_scan_protocol | Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
| Sample_data_processing | Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
| Sample_platform_id | GPL570
| Sample_contact_name | Gerwin,,Heller
| Sample_contact_email | gerwin.heller@meduniwien.ac.at
| Sample_contact_department | Department of Internal Medicine I
| Sample_contact_institute | MUW
| Sample_contact_address | Waehringer Guertel 18-20
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | 1090
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM804nnn/GSM804416/suppl/GSM804416.CEL.gz
| Sample_series_id | GSE32496
| Sample_series_id | GSE32497
| Sample_data_row_count | 54675
| |
|
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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