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Search results for the GEO ID: GSE32653

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GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM810477

GPL1261

Control-1

MIN6 cell line

cell line: MIN6 sample type: sorted cell subpopulations transfection: negative control siRNA

gene expression upon knockdown with negative control siRNA

Sample_geo_accession	GSM810477
Sample_status	Public on Mar 07 2013
Sample_submission_date	Oct 05 2011
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
Sample_growth_protocol_ch1	MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek
Sample_platform_id	GPL1261
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810477/suppl/GSM810477.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810477/suppl/GSM810477.CHP.gz
Sample_series_id	GSE32653
Sample_data_row_count	45101

GSM810478

GPL1261

Control-2

MIN6 cell line

cell line: MIN6 sample type: sorted cell subpopulations transfection: negative control siRNA

gene expression upon knockdown with negative control siRNA

Sample_geo_accession	GSM810478
Sample_status	Public on Mar 07 2013
Sample_submission_date	Oct 05 2011
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
Sample_growth_protocol_ch1	MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek
Sample_platform_id	GPL1261
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810478/suppl/GSM810478.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810478/suppl/GSM810478.CHP.gz
Sample_series_id	GSE32653
Sample_data_row_count	45101

GSM810479

GPL1261

Control-3

MIN6 cell line

cell line: MIN6 sample type: sorted cell subpopulations transfection: negative control siRNA

gene expression upon knockdown with negative control siRNA

Sample_geo_accession	GSM810479
Sample_status	Public on Mar 07 2013
Sample_submission_date	Oct 05 2011
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
Sample_growth_protocol_ch1	MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek
Sample_platform_id	GPL1261
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810479/suppl/GSM810479.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810479/suppl/GSM810479.CHP.gz
Sample_series_id	GSE32653
Sample_data_row_count	45101

GSM810480

GPL1261

HB-1

MIN6 cell line

cell line: MIN6 sample type: sorted cell subpopulations transfection: Hlxb9 siRNA

gene expression upon knockdown with Hlxb9 siRNA

Sample_geo_accession	GSM810480
Sample_status	Public on Mar 07 2013
Sample_submission_date	Oct 05 2011
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
Sample_growth_protocol_ch1	MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek
Sample_platform_id	GPL1261
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810480/suppl/GSM810480.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810480/suppl/GSM810480.CHP.gz
Sample_series_id	GSE32653
Sample_data_row_count	45101

GSM810481

GPL1261

HB-2

MIN6 cell line

cell line: MIN6 sample type: sorted cell subpopulations transfection: Hlxb9 siRNA

gene expression upon knockdown with Hlxb9 siRNA

Sample_geo_accession	GSM810481
Sample_status	Public on Mar 07 2013
Sample_submission_date	Oct 05 2011
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
Sample_growth_protocol_ch1	MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek
Sample_platform_id	GPL1261
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810481/suppl/GSM810481.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810481/suppl/GSM810481.CHP.gz
Sample_series_id	GSE32653
Sample_data_row_count	45101

GSM810482

GPL1261

HB-3

MIN6 cell line

cell line: MIN6 sample type: sorted cell subpopulations transfection: Hlxb9 siRNA

gene expression upon knockdown with Hlxb9 siRNA

Sample_geo_accession	GSM810482
Sample_status	Public on Mar 07 2013
Sample_submission_date	Oct 05 2011
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
Sample_growth_protocol_ch1	MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek
Sample_platform_id	GPL1261
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810482/suppl/GSM810482.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810482/suppl/GSM810482.CHP.gz
Sample_series_id	GSE32653
Sample_data_row_count	45101

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