Search results for the GEO ID: GSE32668  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM810964 | GPL570 |
|
MDA-MB-231_control
|
untreated control (3 hours post change of medium)
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
control sample (3 hours post change of medium)
|
| Sample_geo_accession | GSM810964
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810964/suppl/GSM810964_control.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810965 | GPL570 |
|
MDA-MB-231_control (2)
|
untreated control (3 hours post change of medium)
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
control sample (3 hours post change of medium)
|
| Sample_geo_accession | GSM810965
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810965/suppl/GSM810965_control_2_.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810966 | GPL570 |
|
MDA-MB-231_Ethanol
|
treated for 3 hours with 0.1% Ethanol
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
ethanol treated cells (vehicle for estradiol) 3h
|
| Sample_geo_accession | GSM810966
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810966/suppl/GSM810966_ethanol.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810967 | GPL570 |
|
MDA-MB-231_Estradiol
|
treated for 3 hours with 10-6M estradiol
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
estradiol treated cells 3h
|
| Sample_geo_accession | GSM810967
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810967/suppl/GSM810967_estradiol.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810968 | GPL570 |
|
MDA-MB-231_E2-BSA
|
treated for 3 hours with 10-6M E2-BSA
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
E2-BSA treated cells 3h
|
| Sample_geo_accession | GSM810968
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810968/suppl/GSM810968_E2-BSA.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810969 | GPL570 |
|
MDA-MB-231_E2-BSA (2)
|
treated for 3 hours with 10-6M E2-BSA
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
E2-BSA treated cells 3h
|
| Sample_geo_accession | GSM810969
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810969/suppl/GSM810969_E2-BSA_2_.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810970 | GPL570 |
|
MDA-MB-231_ICI
|
treated for 3 hours with 10-5M ICI
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
ICI treated cells 3h
|
| Sample_geo_accession | GSM810970
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810970/suppl/GSM810970_ICI.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810971 | GPL570 |
|
MDA-MB-231_G15
|
treated for 3 hours with 10-5M G15
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
G15 treated cells 3h
|
| Sample_geo_accession | GSM810971
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810971/suppl/GSM810971_G15.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810972 | GPL570 |
|
MDA-MB-231_E2-BSA+ICI
|
treated for 3 hours with 10-6M E2-BSA plus 10-5M ICI
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
E2-BSA plus ICI treated cells 3h
|
| Sample_geo_accession | GSM810972
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810972/suppl/GSM810972_E2-BSA+ICI.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810973 | GPL570 |
|
MDA-MB-231_E2-BSA+G15
|
treated for 3 hours with 10-6M E2-BSA plus 10-5M G15
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
E2-BSA plus G15 treated cells 3h
|
| Sample_geo_accession | GSM810973
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810973/suppl/GSM810973_E2-BSA+G15.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
| | |
|
GSM810974 | GPL570 |
|
MDA-MB-231_BSA
|
treated for 3 hours with 10-6M BSA
|
cell line: MDA-MB-231
cell type: breast cancer derived cells
|
BSA treated cells 3h
|
| Sample_geo_accession | GSM810974
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Oct 06 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with or without Estradiol, E2-BSA, ICI, G15 or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM810nnn/GSM810974/suppl/GSM810974_BSA.CEL.gz
| | Sample_series_id | GSE32668
| | Sample_series_id | GSE32670
| | Sample_data_row_count | 54675
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