Search results for the GEO ID: GSE32727 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM813078 | GPL570 |
|
HMEC-hTert
|
HMEC, hTert
|
cell line: HMEC immortalized
cell type: expressed hTert
|
immortalized
|
Sample_geo_accession | GSM813078
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 11 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix).
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix).
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813078/suppl/GSM813078_HMEC-hTert_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813078/suppl/GSM813078_HMEC-hTert_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM813079 | GPL570 |
|
HMEC-hTert-Twist1+Ras
|
HMEC, hTert, Twist1+Ras
|
cell line: HMEC-derivatives
cell type: expressed hTert, Twist1 and HRasG12V
|
transformed
|
Sample_geo_accession | GSM813079
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 11 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix).
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix).
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813079/suppl/GSM813079_HMEC-hTert-Ras+Twist1_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813079/suppl/GSM813079_HMEC-hTert-Ras+Twist1_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM813080 | GPL570 |
|
HMEC-hTert-Zeb1+Ras
|
HMEC, hTert, Zeb1+Ras
|
cell line: HMEC-derivatives
cell type: expressed hTert, zeb1 and HRasG12V
|
transformed
|
Sample_geo_accession | GSM813080
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 11 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix).
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix).
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813080/suppl/GSM813080_HMEC-hTert-Ras+Zeb1_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813080/suppl/GSM813080_HMEC-hTert-Ras+Zeb1_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM813081 | GPL570 |
|
HMEC-hTert-Zeb2+Ras
|
HMEC, hTert, Zeb2+Ras
|
cell line: HMEC-derivatives
cell type: expressed hTert, Zeb2 and HRasG12V
|
transformed
|
Sample_geo_accession | GSM813081
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 11 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix).
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix).
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813081/suppl/GSM813081_HMEC-hTert-Ras+Zeb2_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813081/suppl/GSM813081_HMEC-hTert-Ras+Zeb2_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM813082 | GPL570 |
|
MCF7
|
MCF7
|
cell line: MCF7
cell type: luminal cell
|
transformed
|
Sample_geo_accession | GSM813082
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 11 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix).
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix).
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813082/suppl/GSM813082_MCF7_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813082/suppl/GSM813082_MCF7_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM813083 | GPL570 |
|
MDAMB157
|
MDAMB157
|
cell line: MDAMB157
cell type: basal B cell
|
transformed
|
Sample_geo_accession | GSM813083
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 11 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix).
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix).
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813083/suppl/GSM813083_MDAMB157_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM813nnn/GSM813083/suppl/GSM813083_MDAMB157_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM818426 | GPL570 |
|
HMEC-hTert-Twist1+Ras+TGF
|
HMEC, hTert, Twist1+Ras+TGF
|
cell line: HMEC-derivatives
cell type: expressed hTert, Twist1 and HRasG12V
|
transformed + treated TGF
|
Sample_geo_accession | GSM818426
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 17 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix)
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix)
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818426/suppl/GSM818426_HMEC-hTert-Ras+Twist1_TGFb_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818426/suppl/GSM818426_HMEC-hTert-Ras+Twist1_TGFb_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM818427 | GPL570 |
|
HMEC-hTert-Zeb2+Ras+TGF
|
HMEC, hTert, Zeb2+Ras+TGF
|
cell line: HMEC-derivatives
cell type: expressed hTert, Zeb2 and HRasG12V
|
transformed + treated TGF
|
Sample_geo_accession | GSM818427
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Oct 17 2011
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix)
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix)
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818427/suppl/GSM818427_HMEC-hTert-Ras+Zeb2_TGFb_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818427/suppl/GSM818427_HMEC-hTert-Ras+Zeb2_TGFb_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM879254 | GPL570 |
|
HMEC-hTert-Ras
|
HMEC, hTert, Ras
|
cell line: HMEC-derivatives
cell type: expressed HRasG12V
|
|
Sample_geo_accession | GSM879254
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Feb 22 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix)
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix)
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879254/suppl/GSM879254_HMEC-hTert-Ras_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879254/suppl/GSM879254_HMEC-hTert-Ras_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM879255 | GPL570 |
|
HMEC-hTert-Zeb1
|
HMEC, hTert, Zeb1
|
cell line: HMEC-derivatives
cell type: expressed hTert, zeb1
|
|
Sample_geo_accession | GSM879255
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Feb 22 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix)
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix)
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879255/suppl/GSM879255_HMEC-hTert-Zeb1_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879255/suppl/GSM879255_HMEC-hTert-Zeb1_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
| |
|
GSM879256 | GPL570 |
|
HMEC-hTert-Zeb2
|
HMEC, hTert, Zeb2
|
cell line: HMEC-derivatives
cell type: expressed hTert, Zeb2
|
|
Sample_geo_accession | GSM879256
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Feb 22 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix)
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix)
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879256/suppl/GSM879256_HMEC-hTert-Zeb2_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879256/suppl/GSM879256_HMEC-hTert-Zeb2_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
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GSM879257 | GPL570 |
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HMEC-hTert-Twist1
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HMEC, hTert, Twist1
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cell line: HMEC-derivatives
cell type: expressed hTert, Twist1
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Sample_geo_accession | GSM879257
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Feb 22 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | According to manufacturer's instructions (Affymetrix)
| Sample_hyb_protocol | According to manufacturer's instructions (Affymetrix)
| Sample_scan_protocol | The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
| Sample_data_processing | CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | stéphane,,ansieau
| Sample_contact_email | ansieau@lyon.fnclcc.fr
| Sample_contact_phone | 0033-478785160
| Sample_contact_fax | 0033-478782821
| Sample_contact_department | Centre Léon Bérard
| Sample_contact_institute | Inserm U590
| Sample_contact_address | 28 rue Laennec
| Sample_contact_city | Lyon
| Sample_contact_zip/postal_code | 69008
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879257/suppl/GSM879257_HMEC-hTert-Twist1_HG-U133_Plus_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM879nnn/GSM879257/suppl/GSM879257_HMEC-hTert-Twist1_HG-U133_Plus_2_.rma.chp.gz
| Sample_series_id | GSE32727
| Sample_series_id | GSE32905
| Sample_data_row_count | 54675
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