Search results for the GEO ID: GSE32903  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM814498 | GPL1261 |
|
WT-ac-1
|
Ebs at Day2.5 were treated with Activin for 2 h
|
strain: 129
cell type: EBs
genotype/variation: WT
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814498
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814498/suppl/GSM814498.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814499 | GPL1261 |
|
WT-SB-1
|
Ebs at Day2.5 were treated with SB431542 for 2 h
|
strain: 129
cell type: EBs
genotype/variation: WT
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814499
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814499/suppl/GSM814499.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814500 | GPL1261 |
|
Trim33-KD-ac-1
|
Ebs at Day2.5 were treated with Activin for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Trim33-KD
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814500
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814500/suppl/GSM814500.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814501 | GPL1261 |
|
Trim33-KD-SB-1
|
Ebs at Day2.5 were treated with SB431542 for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Trim33-KD
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814501
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814501/suppl/GSM814501.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814502 | GPL1261 |
|
Smad4-null-ac-1
|
Ebs at Day2.5 were treated with Activin for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Smad4-null
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814502
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814502/suppl/GSM814502.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814503 | GPL1261 |
|
Smad4-null-SB-1
|
Ebs at Day2.5 were treated with SB431542 for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Smad4-null
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814503
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814503/suppl/GSM814503.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814504 | GPL1261 |
|
WT-ac-2
|
Ebs at Day2.5 were treated with Activin for 2 h
|
strain: 129
cell type: EBs
genotype/variation: WT
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814504
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814504/suppl/GSM814504.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814505 | GPL1261 |
|
WT-SB-2
|
Ebs at Day2.5 were treated with SB431542 for 2 h
|
strain: 129
cell type: EBs
genotype/variation: WT
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814505
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814505/suppl/GSM814505.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814506 | GPL1261 |
|
Trim33-KD-ac-2
|
Ebs at Day2.5 were treated with Activin for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Trim33-KD
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814506
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814506/suppl/GSM814506.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814507 | GPL1261 |
|
Trim33-KD-SB-2
|
Ebs at Day2.5 were treated with SB431542 for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Trim33-KD
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814507
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814507/suppl/GSM814507.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814508 | GPL1261 |
|
Smad4-null-ac-2
|
Ebs at Day2.5 were treated with Activin for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Smad4-null
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814508
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814508/suppl/GSM814508.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
| | |
|
GSM814509 | GPL1261 |
|
Smad4-null-SB-2
|
Ebs at Day2.5 were treated with SB431542 for 2 h
|
strain: 129
cell type: EBs
genotype/variation: Smad4-null
|
Gene expression data from differentiated mouse ESCs
|
| Sample_geo_accession | GSM814509
| | Sample_status | Public on Jan 09 2012
| | Sample_submission_date | Oct 12 2011
| | Sample_last_update_date | Jan 09 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The Ebs at Day 2.5 were treated with Activin(50ng/ml) or 10 mM SB431542.
| | Sample_growth_protocol_ch1 | Ebs were set up by growing mouse ESCs in medium without LIF in ultra-low attachment dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | preEase RNA spin kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Differentially expressed genes were identified using a Bayes model Implemented by the limma package of R.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Qiaoran,,Xi
| | Sample_contact_email | xiq@mskcc.org
| | Sample_contact_laboratory | Joan Massague
| | Sample_contact_department | Cancer biology and genetics
| | Sample_contact_institute | MSKCC
| | Sample_contact_address | 1275, York ave.
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM814nnn/GSM814509/suppl/GSM814509.CEL.gz
| | Sample_series_id | GSE32903
| | Sample_data_row_count | 45101
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