Search results for the GEO ID: GSE32984  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM816976 | GPL570 |
|
HUVEC_untreated_0 hr_rep1
|
HUVEC untreated
|
cell type: Umbilical vein endothelial cells
|
S0172F016
|
| Sample_geo_accession | GSM816976
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816976/suppl/GSM816976.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816976/suppl/GSM816976.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816977 | GPL570 |
|
HUVEC_untreated_0 hr_rep2
|
HUVEC untreated
|
cell type: Umbilical vein endothelial cells
|
S0172F020
|
| Sample_geo_accession | GSM816977
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816977/suppl/GSM816977.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816977/suppl/GSM816977.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816978 | GPL570 |
|
HUVEC_untreated_0 hr_rep3
|
HUVEC untreated
|
cell type: Umbilical vein endothelial cells
|
S0172F019
|
| Sample_geo_accession | GSM816978
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816978/suppl/GSM816978.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816978/suppl/GSM816978.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816979 | GPL570 |
|
HUVEC_Erg antisense_24hr_rep1
|
HUVEC treated with Erg antisense for 24 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F022
|
| Sample_geo_accession | GSM816979
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816979/suppl/GSM816979.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816979/suppl/GSM816979.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816980 | GPL570 |
|
HUVEC_Erg antisense_24hr_rep2
|
HUVEC treated with Erg antisense for 24 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F021
|
| Sample_geo_accession | GSM816980
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816980/suppl/GSM816980.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816980/suppl/GSM816980.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816981 | GPL570 |
|
HUVEC_Erg antisense_24hr_rep3
|
HUVEC treated with Erg antisense for 24 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F031
|
| Sample_geo_accession | GSM816981
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816981/suppl/GSM816981.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816981/suppl/GSM816981.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816982 | GPL570 |
|
HUVEC_control antisense_24hr_rep1
|
HUVEC treated with control antisense for 24 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F029
|
| Sample_geo_accession | GSM816982
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816982/suppl/GSM816982.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816982/suppl/GSM816982.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816983 | GPL570 |
|
HUVEC_control antisense_24hr_rep2
|
HUVEC treated with control antisense for 24 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F025
|
| Sample_geo_accession | GSM816983
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816983/suppl/GSM816983.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816983/suppl/GSM816983.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816984 | GPL570 |
|
HUVEC_control antisense_24hr_rep3
|
HUVEC treated with control antisense for 24 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F018
|
| Sample_geo_accession | GSM816984
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816984/suppl/GSM816984.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816984/suppl/GSM816984.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816985 | GPL570 |
|
HUVEC_Erg antisense_48hr_rep1
|
HUVEC treated with Erg antisense for 48 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F024
|
| Sample_geo_accession | GSM816985
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816985/suppl/GSM816985.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816985/suppl/GSM816985.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816986 | GPL570 |
|
HUVEC_Erg antisense_48hr_rep2
|
HUVEC treated with Erg antisense for 48 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F023
|
| Sample_geo_accession | GSM816986
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816986/suppl/GSM816986.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816986/suppl/GSM816986.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816987 | GPL570 |
|
HUVEC_Erg antisense_48hr_rep3
|
HUVEC treated with Erg antisense for 48 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F017
|
| Sample_geo_accession | GSM816987
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816987/suppl/GSM816987.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816987/suppl/GSM816987.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816988 | GPL570 |
|
HUVEC_control antisense_48hr_rep1
|
HUVEC treated with control antisense for 48 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F030
|
| Sample_geo_accession | GSM816988
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816988/suppl/GSM816988.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816988/suppl/GSM816988.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816989 | GPL570 |
|
HUVEC_control antisense_48hr_rep2
|
HUVEC treated with control antisense for 48 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F028
|
| Sample_geo_accession | GSM816989
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816989/suppl/GSM816989.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816989/suppl/GSM816989.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
|
GSM816990 | GPL570 |
|
HUVEC_control antisense_48hr_rep3
|
HUVEC treated with control antisense for 48 hours
|
cell type: Umbilical vein endothelial cells
|
S0172F027
|
| Sample_geo_accession | GSM816990
| | Sample_status | Public on Feb 27 2012
| | Sample_submission_date | Oct 14 2011
| | Sample_last_update_date | Feb 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HUVEC were treated with either Erg-specific or control antisense (known as GeneBloc) for either 24 hr or 48 hr
| | Sample_growth_protocol_ch1 | HUVEC were maintained in EGM-2 media
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using the Rneasy kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 2ug of total RNA is converted to cDNA via first and second strand synthesis using the GeneChip® Expression 3’-Amplification One-Cycle cDNA Synthesis kit, in conjunction with the GeneChip® Eukaryotic PolyA RNA Control Kit. Biotin labelled cRNA is synthesised from the double-stranded cDNA using the GeneChip® Expression 3’-Amplification IVT Labelling Kit
| | Sample_hyb_protocol | 15µg of fragmented cRNA was made up into a hybridisation cocktail in accordance with the Affymetrix technical manual corresponding to a 49 format (standard)/64 format array. The hybridisation cocktail was added to the appropriate array and hybridised for 16hrs at 45°C. The array is washed and stained on the GeneChip® fluidics station 450 using the appropriate fluidics script.
| | Sample_scan_protocol | The array was inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
| | Sample_data_processing | Differential gene expression analysis was carried out using Rosetta Resolver Data Analysis System (version 7.1, Rosetta Biosoftware). Statistical analysis was performed using false discovery rate (FDR)–corrected P values, following Benjamin-Hochberg Multiple Test Correction.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Graeme,Miles,Birdsey
| | Sample_contact_email | g.birdsey@imperial.ac.uk
| | Sample_contact_phone | +442083838048
| | Sample_contact_fax | +442083831640
| | Sample_contact_department | NHLI Cardiovascular Sciences
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Hammersmith Hospital, Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816990/suppl/GSM816990.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM816nnn/GSM816990/suppl/GSM816990.CHP.gz
| | Sample_series_id | GSE32984
| | Sample_data_row_count | 54675
| | |
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