Search results for the GEO ID: GSE33031  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM818694 | GPL1261 |
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PU.1 ki/ki rep1
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sorted HSCs (lin-sca1+ckit+CD150+CD48-) of PU.1ki/ki mice
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tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: knock-in mice
age: adult
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Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Sample_geo_accession | GSM818694
| | Sample_status | Public on Dec 01 2011
| | Sample_submission_date | Oct 18 2011
| | Sample_last_update_date | Dec 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Henry,,Yang
| | Sample_contact_email | csiyangh@nus.edu.sg
| | Sample_contact_institute | Cancer Science Institute of Singapore
| | Sample_contact_address | 28 Medical Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 117456
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818694/suppl/GSM818694_PU1kiki_1.CEL.gz
| | Sample_series_id | GSE33031
| | Sample_data_row_count | 45101
| | |
|
GSM818695 | GPL1261 |
|
PU.1 ki/ki rep2
|
sorted HSCs (lin-sca1+ckit+CD150+CD48-) of PU.1ki/ki mice
|
tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: knock-in mice
age: adult
|
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Sample_geo_accession | GSM818695
| | Sample_status | Public on Dec 01 2011
| | Sample_submission_date | Oct 18 2011
| | Sample_last_update_date | Dec 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Henry,,Yang
| | Sample_contact_email | csiyangh@nus.edu.sg
| | Sample_contact_institute | Cancer Science Institute of Singapore
| | Sample_contact_address | 28 Medical Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 117456
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818695/suppl/GSM818695_PU1kiki_2.CEL.gz
| | Sample_series_id | GSE33031
| | Sample_data_row_count | 45101
| | |
|
GSM818696 | GPL1261 |
|
PU.1 ki/ki rep3
|
sorted HSCs (lin-sca1+ckit+CD150+CD48-) of PU.1ki/ki mice
|
tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: knock-in mice
age: adult
|
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Sample_geo_accession | GSM818696
| | Sample_status | Public on Dec 01 2011
| | Sample_submission_date | Oct 18 2011
| | Sample_last_update_date | Dec 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Henry,,Yang
| | Sample_contact_email | csiyangh@nus.edu.sg
| | Sample_contact_institute | Cancer Science Institute of Singapore
| | Sample_contact_address | 28 Medical Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 117456
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818696/suppl/GSM818696_PU1kiki_3.CEL.gz
| | Sample_series_id | GSE33031
| | Sample_data_row_count | 45101
| | |
|
GSM818697 | GPL1261 |
|
WT rep1
|
sorted HSCs (lin-sca1+ckit+CD150+CD48-) of wild type mice
|
tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: wild type mice
age: adult
|
Gene expression data from embryos in slow phase of cellularisation.
|
| Sample_geo_accession | GSM818697
| | Sample_status | Public on Dec 01 2011
| | Sample_submission_date | Oct 18 2011
| | Sample_last_update_date | Dec 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Henry,,Yang
| | Sample_contact_email | csiyangh@nus.edu.sg
| | Sample_contact_institute | Cancer Science Institute of Singapore
| | Sample_contact_address | 28 Medical Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 117456
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818697/suppl/GSM818697_WT_1.CEL.gz
| | Sample_series_id | GSE33031
| | Sample_data_row_count | 45101
| | |
|
GSM818698 | GPL1261 |
|
WT rep2
|
sorted HSCs (lin-sca1+ckit+CD150+CD48-) of wild type mice
|
tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: wild type mice
age: adult
|
Gene expression data from embryos in slow phase of cellularisation.
|
| Sample_geo_accession | GSM818698
| | Sample_status | Public on Dec 01 2011
| | Sample_submission_date | Oct 18 2011
| | Sample_last_update_date | Dec 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Henry,,Yang
| | Sample_contact_email | csiyangh@nus.edu.sg
| | Sample_contact_institute | Cancer Science Institute of Singapore
| | Sample_contact_address | 28 Medical Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 117456
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818698/suppl/GSM818698_WT_2.CEL.gz
| | Sample_series_id | GSE33031
| | Sample_data_row_count | 45101
| | |
|
GSM818699 | GPL1261 |
|
WT rep3
|
sorted HSCs (lin-sca1+ckit+CD150+CD48-) of wild type mice
|
tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: wild type mice
age: adult
|
Gene expression data from embryos in slow phase of cellularisation.
|
| Sample_geo_accession | GSM818699
| | Sample_status | Public on Dec 01 2011
| | Sample_submission_date | Oct 18 2011
| | Sample_last_update_date | Dec 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Henry,,Yang
| | Sample_contact_email | csiyangh@nus.edu.sg
| | Sample_contact_institute | Cancer Science Institute of Singapore
| | Sample_contact_address | 28 Medical Drive
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 117456
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM818nnn/GSM818699/suppl/GSM818699_WT_3.CEL.gz
| | Sample_series_id | GSE33031
| | Sample_data_row_count | 45101
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